FastQCFastQC Report
Thu 26 May 2016
SRR1926807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752344
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9890.13145582339993406No Hit
GTATCAACGCAGAGTACTTTTTTTT8780.11670193422157948No Hit
GTCCTACAGTGTGCATTTCTCATTT7900.1050051572153164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGCC350.002194563916.25694719
TAGGACC2050.015.3237194
CTAGAGT551.9246343E-413.8462284
TAGGGGA655.342543E-513.1805424
GTATTAG603.962106E-412.7135791
TTAGGGT604.0183155E-412.6923744
GTAGGAC4850.012.5766273
GTCCTAA2250.012.2897941
GTAGTAC802.7686865E-511.9134073
CTAGACT802.8029577E-511.8991014
TTACACA1209.680662E-911.89914
TCCTATA1052.6011003E-711.7999462
GGCGAGG1850.011.78994719
GTCCTAC5200.011.5522431
GTATAAG1001.8412538E-611.4422221
TGGCGAG4150.011.42556418
CAATACT752.0324482E-411.4231374
CTACACT1354.5602064E-911.282114
GTCCTAT1104.723206E-711.2688541
GTGTTAT855.1176805E-511.2178641
Produced by FastQC (version 0.11.5)