##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926807_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 752344 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.364579235030785 31.0 27.0 31.0 16.0 33.0 2 28.02690391629361 31.0 28.0 31.0 16.0 34.0 3 27.847430164924557 31.0 28.0 31.0 16.0 34.0 4 31.5605029082441 35.0 32.0 37.0 19.0 37.0 5 31.353699903235754 35.0 32.0 37.0 17.0 37.0 6 31.009510277213614 35.0 32.0 37.0 11.0 37.0 7 31.066336675775975 35.0 32.0 37.0 13.0 37.0 8 30.737940357070702 35.0 31.0 37.0 11.0 37.0 9 32.90015072892188 37.0 32.0 39.0 17.0 39.0 10 33.029971661899346 37.0 33.0 39.0 17.0 39.0 11 33.209929234499114 37.0 33.0 39.0 17.0 39.0 12 33.04617036887381 37.0 33.0 39.0 17.0 39.0 13 33.17652563189179 38.0 33.0 39.0 17.0 39.0 14 34.178221664557704 39.0 34.0 40.0 17.0 41.0 15 33.71535494401497 39.0 33.0 40.0 10.0 41.0 16 33.79809236200462 39.0 33.0 40.0 11.0 41.0 17 34.03911907318992 39.0 33.0 40.0 17.0 41.0 18 34.0975683995619 39.0 34.0 41.0 16.0 41.0 19 33.446218750996884 39.0 33.0 40.0 10.0 41.0 20 33.71056458215922 39.0 33.0 40.0 10.0 41.0 21 33.71691407122274 39.0 34.0 40.0 8.0 41.0 22 33.744721829375926 39.0 34.0 40.0 2.0 41.0 23 33.72617579192497 39.0 34.0 41.0 2.0 41.0 24 32.37265665706113 38.0 32.0 40.0 2.0 41.0 25 32.959268632434096 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7053791365602926 1101 2 -0.6513696228284829 1101 3 -1.504406982245662 1101 4 -0.8125545309458921 1101 5 -1.0417183348495342 1101 6 -2.768709919982907 1101 7 -0.9082712159169724 1101 8 -0.6647375806919129 1101 9 -0.4571702806179445 1101 10 -9.574774808912967 1101 11 -4.333485268057103 1101 12 -1.8863748592747527 1101 13 -1.339344514863626 1101 14 -0.9633939131617808 1101 15 -2.5205987268500287 1101 16 -0.9205403615707795 1101 17 -0.8119279424904775 1101 18 -0.5846536830187716 1101 19 -1.7226506657377456 1101 20 -0.7081768934389956 1101 21 -0.8453359150595929 1101 22 -0.4876640127973033 1101 23 -0.8433204700132251 1101 24 -0.7047387497609812 1101 25 -0.6015937259384501 1102 1 -0.7544918747079556 1102 2 -0.5359441226599166 1102 3 -0.5810594378825691 1102 4 -0.6427182078119174 1102 5 -0.8256808409589844 1102 6 -1.1388015899966675 1102 7 -0.22439968620688688 1102 8 0.2579752258493535 1102 9 0.18602399109500567 1102 10 0.1761450366852415 1102 11 -0.11257553632457018 1102 12 -0.07328135521549228 1102 13 -0.4173390336968694 1102 14 -0.48338291211491224 1102 15 -0.022624726098342762 1102 16 0.01847238319042077 1102 17 -0.28165570690676844 1102 18 -0.47024118247436775 1102 19 -0.018663512121513293 1102 20 -0.22976443160794702 1102 21 -0.3255058021940158 1102 22 -0.2922365446995272 1102 23 -0.9500935339015655 1102 24 -4.2080713249576505 1102 25 -2.5965682477074523 1103 1 -0.5094961234477076 1103 2 -0.3235901930548266 1103 3 0.01756882613986832 1103 4 -0.039267783896306696 1103 5 -0.5174092155125471 1103 6 0.6386714640485813 1103 7 0.691516795708818 1103 8 0.37078086357560736 1103 9 0.28188897700707827 1103 10 0.1768015148952813 1103 11 0.014404280564335181 1103 12 -0.09932165754687361 1103 13 -0.00904632427872798 1103 14 -0.011387857264637091 1103 15 0.1299114201349738 1103 16 0.3362765194823254 1103 17 0.06169836964721753 1103 18 -0.01791811213696093 1103 19 -1.8350540639465542 1103 20 -0.5636339154339893 1103 21 -0.37647630380087094 1103 22 -0.26367095757480996 1103 23 -0.32588018966419696 1103 24 1.3861532640646246 1103 25 0.7458993436723986 1104 1 -0.6192561524933637 1104 2 -0.23007131807321457 1104 3 -1.342032359473766 1104 4 -0.43107349725198674 1104 5 -0.27568628853670774 1104 6 -1.6198716521622138 1104 7 -0.2987820177385707 1104 8 0.2900993548290458 1104 9 0.3558834120095966 1104 10 0.3254828796655289 1104 11 0.12671049940207268 1104 12 0.07393114294890779 1104 13 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-0.19939842696972931 2215 1 0.9263021221411734 2215 2 0.04947882200305642 2215 3 0.48751328030236607 2215 4 0.791233533775987 2215 5 0.9794084410094257 2215 6 1.4439319731661833 2215 7 0.9205838722542481 2215 8 -0.24615215737810558 2215 9 0.22482616748313689 2215 10 0.08933689418130086 2215 11 0.011366035090155435 2215 12 -0.34920408504621747 2215 13 0.09547061046280447 2215 14 0.12986044725387558 2215 15 0.32155172857639513 2215 16 0.5135493249629235 2215 17 -0.12250117669498906 2215 18 -0.762488269942196 2215 19 0.36996513478251813 2215 20 -3.915437362889115 2215 21 -1.9761856703145 2215 22 -1.003060039390931 2215 23 -0.9327521589462293 2215 24 -0.8902331594659323 2215 25 -0.42303370022229103 2216 1 0.2476171504070912 2216 2 -0.6461455863737626 2216 3 0.05015525415214839 2216 4 -0.14999196375318036 2216 5 0.07178776873692883 2216 6 -1.5508303153108507 2216 7 -9.801596167243865 2216 8 -11.136306039117581 2216 9 -5.823306974603227 2216 10 -3.2146533959701955 2216 11 -2.15892087308238 2216 12 -1.788978887964202 2216 13 -1.5403711004014404 2216 14 -1.5993134739334351 2216 15 -2.5895743699727873 2216 16 -1.5764113403452384 2216 17 -1.7431036800860156 2216 18 -1.573745861261159 2216 19 -0.8608367322639623 2216 20 -2.025805178085207 2216 21 -4.665666625862151 2216 22 -3.0926970011788306 2216 23 -3.276813173602772 2216 24 -3.2237797809440067 2216 25 -2.450948886128682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45842.0 3 5.0 4 398.0 5 2019.0 6 2682.0 7 2113.0 8 1724.0 9 1573.0 10 1482.0 11 1497.0 12 1495.0 13 1430.0 14 1450.0 15 1503.0 16 1636.0 17 1684.0 18 2034.0 19 2424.0 20 3263.0 21 4017.0 22 5265.0 23 6107.0 24 7128.0 25 8292.0 26 9613.0 27 10309.0 28 11798.0 29 13720.0 30 16499.0 31 20059.0 32 25662.0 33 33224.0 34 44667.0 35 61711.0 36 89303.0 37 140574.0 38 168142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79468519248596 17.97676636370417 12.25053542317077 25.978013020639096 2 14.62588881598997 19.68204242362138 41.39996087413048 24.29210788625817 3 18.9634223431792 23.937238410199424 29.533080920663018 27.56625832595836 4 11.733316308479708 16.178133171597224 38.38944884696519 33.69910167295788 5 13.214365006667208 37.64399638920538 36.070987477881225 13.070651126246197 6 32.63564005514542 36.94783120821385 18.047628733583004 12.368900003057725 7 28.126233162334824 30.909580538165375 22.770842443181213 18.19334385631859 8 24.865162458242356 36.99974138919327 20.59435162005007 17.54074453251431 9 25.957378923438934 15.609071934699086 19.87942196992861 38.554127171933374 10 14.03045960658336 29.36623594893083 35.01658072699907 21.58672371748675 11 34.74503381248197 22.644326189751368 24.14013136817253 18.470508629594132 12 22.916486645653507 24.941407314547337 31.73908939721653 20.403016642582624 13 28.882519737795402 21.916364124604346 25.586749920570245 23.614366217030007 14 20.51034922829454 22.095236829161294 27.631442510934157 29.762971431610012 15 23.10443062795035 30.851150096843966 23.008051940800886 23.036367334404797 16 21.99956129564021 28.444128340966344 27.549437328423387 22.006873034970056 17 21.5610081638459 28.800522188352062 27.665347458652334 21.973122189149702 18 21.46282128827709 28.055088435283714 29.077494699200397 21.4045955772388 19 23.709389869220654 27.234994675497852 27.034378602015735 22.021236853265762 20 23.158404100884304 27.7204517779184 27.839298723259436 21.28184539793786 21 22.94063378203407 26.985454226409978 27.189111008898685 22.884800982657268 22 22.689663240400517 28.045353270550947 27.337323258373793 21.927660230674743 23 22.146860509635427 27.86523695069554 27.519861105202704 22.46804143446633 24 23.12882938093143 27.196796779763986 27.808168371124758 21.86620546817983 25 22.494665966540158 27.439065729915118 27.781640290065074 22.28462801347965 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 242.0 1 242.0 2 317.0 3 392.0 4 392.0 5 392.0 6 571.5 7 751.0 8 751.0 9 751.0 10 1070.5 11 1390.0 12 1390.0 13 1390.0 14 2352.5 15 3315.0 16 3315.0 17 3315.0 18 5728.5 19 8142.0 20 8142.0 21 8142.0 22 13010.5 23 17879.0 24 17879.0 25 17879.0 26 25518.5 27 33158.0 28 33158.0 29 33158.0 30 43353.0 31 53548.0 32 53548.0 33 53548.0 34 62085.5 35 70623.0 36 70623.0 37 70623.0 38 77817.0 39 85011.0 40 85011.0 41 85011.0 42 89864.0 43 94717.0 44 94717.0 45 94717.0 46 97277.5 47 99838.0 48 99838.0 49 99838.0 50 96472.5 51 93107.0 52 93107.0 53 93107.0 54 83554.5 55 74002.0 56 74002.0 57 74002.0 58 63076.5 59 52151.0 60 52151.0 61 52151.0 62 42502.0 63 32853.0 64 32853.0 65 32853.0 66 25403.5 67 17954.0 68 17954.0 69 17954.0 70 13170.0 71 8386.0 72 8386.0 73 8386.0 74 5791.5 75 3197.0 76 3197.0 77 3197.0 78 2157.0 79 1117.0 80 1117.0 81 1117.0 82 761.5 83 406.0 84 406.0 85 406.0 86 262.0 87 118.0 88 118.0 89 118.0 90 75.0 91 32.0 92 32.0 93 32.0 94 20.0 95 8.0 96 8.0 97 8.0 98 7.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20416192592750126 2 0.12268324064523677 3 0.12348074816839105 4 0.06672479610390991 5 0.020602277681486127 6 0.020070605999383262 7 0.297736141977606 8 0.29002690258711444 9 0.01781100135044607 10 0.035356166859840714 11 0.014222217496251714 12 0.015551396701508886 13 0.014222217496251714 14 0.015152642939931734 15 0.013690545814148847 16 0.01714641174781749 17 0.01661474006571462 18 0.01315887413204598 19 0.02139978520464043 20 0.015418478780983166 21 0.013025956211520261 22 0.015418478780983166 23 0.015950150463086038 24 0.013424709973097412 25 0.01315887413204598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 752344.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.56978675626799 #Duplication Level Percentage of deduplicated Percentage of total 1 93.38175864428509 81.7742069140533 2 5.070346853643032 8.880183855076693 3 0.8251774388144496 2.1678183705919416 4 0.26703584505143896 0.9353708802973731 5 0.12523626536086074 0.5483456525900984 6 0.07528444897901199 0.395558588589313 7 0.04970782859139828 0.30470327649061074 8 0.035450632084232055 0.248352343359289 9 0.027131259755776523 0.2138290768118039 >10 0.12421116653070896 1.9179832757972384 >50 0.008951212272985672 0.5189260476544583 >100 0.008343160254696724 1.2541231436229685 >500 0.0013652443763390082 0.8405985750649334 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 989 0.13145582339993406 No Hit GTATCAACGCAGAGTACTTTTTTTT 878 0.11670193422157948 No Hit GTCCTACAGTGTGCATTTCTCATTT 790 0.1050051572153164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.9875376157715085E-4 0.0 6 0.0 0.0 0.0 3.9875376157715085E-4 0.0 7 0.0 0.0 0.0 3.9875376157715085E-4 0.0 8 0.0 0.0 0.0 3.9875376157715085E-4 0.0 9 0.0 0.0 0.0 3.9875376157715085E-4 0.0 10 0.0 0.0 0.0 3.9875376157715085E-4 0.0 11 0.0 0.0 0.0 3.9875376157715085E-4 0.0 12 0.0 0.0 0.0 3.9875376157715085E-4 0.0 13 0.0 0.0 0.0 3.9875376157715085E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCACGCC 35 0.0021945639 16.256947 19 TAGGACC 205 0.0 15.323719 4 CTAGAGT 55 1.9246343E-4 13.846228 4 TAGGGGA 65 5.342543E-5 13.180542 4 GTATTAG 60 3.962106E-4 12.713579 1 TTAGGGT 60 4.0183155E-4 12.692374 4 GTAGGAC 485 0.0 12.576627 3 GTCCTAA 225 0.0 12.289794 1 GTAGTAC 80 2.7686865E-5 11.913407 3 CTAGACT 80 2.8029577E-5 11.899101 4 TTACACA 120 9.680662E-9 11.8991 4 TCCTATA 105 2.6011003E-7 11.799946 2 GGCGAGG 185 0.0 11.789947 19 GTCCTAC 520 0.0 11.552243 1 GTATAAG 100 1.8412538E-6 11.442222 1 TGGCGAG 415 0.0 11.425564 18 CAATACT 75 2.0324482E-4 11.423137 4 CTACACT 135 4.5602064E-9 11.28211 4 GTCCTAT 110 4.723206E-7 11.268854 1 GTGTTAT 85 5.1176805E-5 11.217864 1 >>END_MODULE