FastQCFastQC Report
Thu 26 May 2016
SRR1926807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752344
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14470.19233223100071245No Hit
GTCCTACAGTGGACATTTCTAAATT9490.1261391065789054No Hit
CTGTAGGACGTGGAATATGGCAAGA8820.11723360590368236No Hit
GGTATCAACGCAGAGTACTTTTTTT8450.11231564284423083No Hit
TATCAACGCAGAGTACTTTTTTTTT8080.10739767978477929No Hit
GTCCTACAGTGTGCATTTCTCATTT7820.10394181385111065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT500.001473432613.3295544
CCGTGAT500.001510874313.28524919
GGCATAT655.5056124E-513.13838413
TTATTCT951.0099375E-612.0266654
CTCTAGG1302.4774636E-911.7292411
TAGACTG907.2643215E-611.6369115
TAAACTG907.2643215E-611.6369115
GGCCTCG752.0925592E-411.38735715
GTCCTAC5550.011.3329841
GTCCTAA2550.011.2117751
TAGGACC2850.011.0244444
TGTGCAA1303.3105607E-810.94865310
GATCTAC700.001451150610.8914381
AGACTAT700.001462508810.88126856
TTAGGAC3150.010.88126853
GGCGAGA2900.010.79835519
GGACTGT1158.543793E-710.7629946
GTCTAAG803.6575896E-410.721261
TATGGAC803.6893148E-410.7112493
TTATATC803.6893148E-410.7112494
Produced by FastQC (version 0.11.5)