FastQCFastQC Report
Thu 26 May 2016
SRR1926806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628044
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13120.208902560967066No Hit
GTCCTACAGTGGACATTTCTAAATT9970.1587468393934183No Hit
GTACATGGGAAGCAGTGGTATCAAC9110.14505353128124782No Hit
GTCCTACAGTGTGCATTTCTCATTT8710.13868455076395922No Hit
GGTATCAACGCAGAGTACTTTTTTT8340.13279324378546725No Hit
CCCATGTACTCTGCGTTGATACCAC7200.11464164931119475No Hit
TATCAACGCAGAGTACTTTTTTTTT7050.11225328161721153No Hit
CTGTAGGACGTGGAATATGGCAAGA6920.11018336294909274No Hit
CTGTAGGACCTGGAATATGGCGAGA6390.10174446376368534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTAG508.367821E-515.2696541
GTCAGTC508.546955E-515.2317987
TAGGACC2650.014.0171074
TCTAGGA1204.7293724E-1113.5102582
CTAGGAC852.5670488E-713.463513
CTGTAGG5450.012.6079711
GTAGGAC5500.012.4843453
TGTAGGA5850.012.3894982
GGCGAGA2850.012.30794919
CTACCCT701.0630781E-412.2457134
GTAAGAC550.002977773312.1375583
TGGCGAG4400.012.06602418
AGGACCT3750.011.9315745
TAGGACA2250.011.8526414
GGTTATA657.710108E-411.7458891
GTCCTAA2450.011.6859611
GGACTTG907.2694183E-611.6354026
GTCCTAC5900.011.1610831
GTCTTAT600.005672583411.1341231
TCCTACA6000.011.1260952
Produced by FastQC (version 0.11.5)