FastQCFastQC Report
Thu 26 May 2016
SRR1926806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628044
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23970.3816611574985192No Hit
GGTATCAACGCAGAGTACTTTTTTT14760.23501538108794928No Hit
TATCAACGCAGAGTACTTTTTTTTT12590.20046366178165861No Hit
GTACATGGGAAGCAGTGGTATCAAC11530.18358586341084382No Hit
GTCCTACAGTGGACATTTCTAAATT10360.16495659539777469No Hit
GTCCTACAGTGTGCATTTCTCATTT9460.15062638923387534No Hit
CCCATGTACTCTGCGTTGATACCAC8940.14234671456140016No Hit
CTGTAGGACGTGGAATATGGCAAGA8490.1351816114794505No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8470.13486316245358607No Hit
CTGTAGGACCTGGAATATGGCGAGA7520.11973683372502565No Hit
GTCCTAAAGTGTGTATTTCTCATTT7250.11543777187585584No Hit
CACACACACACACACACACACACAC6630.10556585207405851No Hit
CTTTAGGACGTGAAATATGGCGAGG6410.10206291278954976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT350.002134622316.3238683
CCTCGAA350.002181789816.27055517
GTGCGCC350.002187601516.26407811
TTGGACT400.00520145514.2799624
TAAGATA400.00520145514.2799624
GCCTCGA400.00530714814.23673616
TGCGCCA400.00532118914.23106912
TAGGACC2700.014.1036664
GATATAC1750.013.6173711
GTTACAA2000.013.28762215
GCCCTGA751.4248562E-512.7095461
GTAGACT853.8414237E-612.3199664
CCAACGA853.973899E-612.28267519
CGCCACC550.0030956112.07484614
GTCCTAT959.950054E-712.0406241
CTAGGAC802.7922762E-511.9028213
GTAGGAC5550.011.8384823
ACTGTTC2350.011.7068998
AGGACCT4150.011.6992465
TTATACT907.2686E-611.6355244
Produced by FastQC (version 0.11.5)