##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926806_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628044 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.996234340269154 31.0 30.0 33.0 26.0 34.0 2 30.302604594582544 31.0 30.0 34.0 26.0 34.0 3 30.346697046703735 31.0 30.0 34.0 26.0 34.0 4 33.947882632427024 37.0 35.0 37.0 30.0 37.0 5 33.94246103776169 37.0 35.0 37.0 30.0 37.0 6 33.90820866053971 37.0 35.0 37.0 30.0 37.0 7 33.89857876199757 37.0 35.0 37.0 30.0 37.0 8 33.932800886562084 37.0 35.0 37.0 30.0 37.0 9 35.46037857220195 39.0 35.0 39.0 30.0 39.0 10 35.36122946799906 39.0 35.0 39.0 30.0 39.0 11 35.4011597913522 39.0 35.0 39.0 30.0 39.0 12 35.29777531510531 39.0 35.0 39.0 30.0 39.0 13 35.31516581640777 39.0 35.0 39.0 30.0 39.0 14 36.418590098782886 40.0 37.0 41.0 30.0 41.0 15 36.4165488405271 40.0 37.0 41.0 30.0 41.0 16 36.39686232174816 40.0 37.0 41.0 30.0 41.0 17 36.38463706364522 40.0 37.0 41.0 30.0 41.0 18 36.3558938545707 40.0 37.0 41.0 30.0 41.0 19 36.42525523689423 40.0 37.0 41.0 30.0 41.0 20 36.37929189674609 40.0 37.0 41.0 30.0 41.0 21 36.337290062479696 40.0 37.0 41.0 30.0 41.0 22 36.2594483825974 40.0 37.0 41.0 29.0 41.0 23 36.19727439478763 40.0 37.0 41.0 29.0 41.0 24 36.1314318741999 40.0 37.0 41.0 29.0 41.0 25 36.024186203514404 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.15777786506255964 1101 2 -0.1867482283627382 1101 3 0.12058670279027695 1101 4 -0.09864549290557534 1101 5 -0.1788291496582275 1101 6 0.0769001992686853 1101 7 -0.03420875252944455 1101 8 -0.08142411138035044 1101 9 0.13417881083516647 1101 10 0.1153396943080125 1101 11 0.10766795115044658 1101 12 0.04126696696940968 1101 13 0.2537440929096775 1101 14 0.24550499552731253 1101 15 0.187941434659038 1101 16 0.26292060081941315 1101 17 0.06300960951835322 1101 18 -0.19343663017699697 1101 19 -0.12616724717333483 1101 20 -0.19717397004003345 1101 21 -0.3174796932013493 1101 22 -0.18693277161408162 1101 23 -0.018151856167008873 1101 24 -0.1595630167905071 1101 25 -0.11088123439731845 1102 1 -0.7209470545977581 1102 2 -0.6941026933104482 1102 3 -0.476326908726346 1102 4 -0.48124806167235334 1102 5 -0.6639383153386191 1102 6 -0.6698727241426425 1102 7 -0.4541662007097145 1102 8 -0.5612603513468244 1102 9 -0.42243491166617986 1102 10 -0.47621579575172035 1102 11 -0.6206359167362834 1102 12 -0.6408308745849283 1102 13 -0.7047356466216073 1102 14 -0.6894538111796678 1102 15 -0.6222447525781831 1102 16 -0.5172789874779156 1102 17 -0.4282520703574946 1102 18 -0.5742339702277448 1102 19 -0.8031280156849903 1102 20 -0.6491212684139427 1102 21 -0.7228617615697814 1102 22 -0.703048582562424 1102 23 -0.7737759264972439 1102 24 -0.4685127144607506 1102 25 -0.5608413996310091 1103 1 -0.3416522028321509 1103 2 -0.3260220658430093 1103 3 -0.05042015761279117 1103 4 -0.18553304939076298 1103 5 -0.08652892202883322 1103 6 -0.21414119956006772 1103 7 -0.292487054787955 1103 8 -0.031005665069137933 1103 9 -0.07588641590486844 1103 10 0.017224897947379247 1103 11 -0.11851258079533977 1103 12 -0.13141840688540185 1103 13 -0.08187979399161804 1103 14 -0.08651307072612724 1103 15 -0.12787239106826576 1103 16 -0.1400903663690798 1103 17 0.07692855003976007 1103 18 -0.012904684384764664 1103 19 0.10049511157148316 1103 20 0.1036469367399917 1103 21 0.08197948998964932 1103 22 -0.1468668469006431 1103 23 -0.019175175233165476 1103 24 -0.06366484885811019 1103 25 -0.06521351026342614 1104 1 -0.36879314312979616 1104 2 -0.33960104270135716 1104 3 -0.1983529704867344 1104 4 -0.26979030589754416 1104 5 -0.41607204367364403 1104 6 -0.3025826229253923 1104 7 -0.4392538813429496 1104 8 -0.4416370103655538 1104 9 -0.41701794437909 1104 10 -0.44207730234139575 1104 11 -0.21750799511179508 1104 12 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10 0.682404197575643 1107 11 0.730197723889539 1107 12 0.7161178865873481 1107 13 0.7519997657649142 1107 14 0.9744603927571873 1107 15 0.4949105091472177 1107 16 0.5668994002022885 1107 17 0.7992716789620786 1107 18 0.6822784356090565 1107 19 0.6034923815125026 1107 20 0.8213852374070498 1107 21 0.776495119148052 1107 22 0.931670639899778 1107 23 0.7064893108203592 1107 24 0.4859333688769709 1107 25 0.8468172071926432 1108 1 0.6433834319908378 1108 2 0.5408120914884194 1108 3 0.6008709521420315 1108 4 0.9137966650247407 1108 5 0.6606269121037371 1108 6 0.7372811255677263 1108 7 0.6770438529447134 1108 8 0.6528707750437945 1108 9 0.5066936938251985 1108 10 0.42858778326471025 1108 11 0.45421937924371036 1108 12 0.45196794004201735 1108 13 0.4422206702675311 1108 14 0.5086030048255097 1108 15 0.5128644386865133 1108 16 0.6298919911373773 1108 17 0.6845741329040465 1108 18 0.7801097923629925 1108 19 0.7706407617119027 1108 20 0.7392546339909245 1108 21 0.7257162971396482 1108 22 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-0.34046609718527066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13789.0 3 10.0 4 198.0 5 1516.0 6 2218.0 7 1663.0 8 1278.0 9 1088.0 10 963.0 11 899.0 12 769.0 13 810.0 14 741.0 15 714.0 16 762.0 17 826.0 18 904.0 19 1126.0 20 1428.0 21 1828.0 22 2278.0 23 2883.0 24 3332.0 25 4081.0 26 4820.0 27 5165.0 28 6059.0 29 7420.0 30 9438.0 31 12239.0 32 16028.0 33 21567.0 34 29453.0 35 41268.0 36 61248.0 37 107046.0 38 260189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87898158214389 19.150489538134877 10.419303520019895 27.551225359701338 2 14.065172719983037 20.935712042494107 40.53774428610827 24.461370951414587 3 18.973831394682193 24.752678651055536 28.991381177459836 27.28210877680244 4 11.508429345714632 17.40865289693079 38.118348395972255 32.964569361382324 5 12.329220832988325 39.08462366481742 35.60677957465239 12.979375927541865 6 31.38561926506561 38.440816835202966 17.692077861695314 12.481486038036106 7 28.118604358552503 32.140143883517105 21.24997411880504 18.491277639125357 8 24.834069034925125 38.808577452497886 19.460103316708388 16.897250195868605 9 26.24475893071836 15.768512340498717 19.695751250451853 38.29097747833107 10 13.855264373087225 30.898992697615018 34.37041212457047 20.875330804727284 11 34.507895612628516 23.210410075046934 23.57394105479716 18.707753257527386 12 22.811299845233773 25.915222500334373 31.915120596646098 19.35835705778576 13 29.075171843876436 22.294245120939557 25.55915935424598 23.07142368093803 14 20.22727743164083 23.02995177081824 27.95063395340765 28.79213684413328 15 22.881523258739346 31.68710650263844 23.321475999248435 22.10989423937378 16 21.17568757453733 29.574343076327818 28.325857706742074 20.924111642392774 17 20.952035207724297 29.603817566922064 27.987847985173016 21.456299240180623 18 20.50324659815745 28.936599781543272 30.087955901038466 20.472197719260816 19 22.354997985806705 27.903344197769897 28.2972662699847 21.4443915464387 20 22.418405138509844 28.18028093662526 28.984208062518114 20.417105862346784 21 22.259308684283443 27.39035164548173 27.950735872552794 22.399603797682026 22 21.821719497360057 28.811038717032567 28.06523109845807 21.30201068714931 23 21.39133563890428 28.511218959181207 28.47539344377146 21.62205195814306 24 22.036672589818547 27.801077631503524 29.13697129500481 21.02527848367312 25 21.7259279310839 27.98028693810608 28.792853616980622 21.500931513829396 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 347.0 1 347.0 2 345.0 3 343.0 4 343.0 5 343.0 6 565.0 7 787.0 8 787.0 9 787.0 10 1230.5 11 1674.0 12 1674.0 13 1674.0 14 2473.5 15 3273.0 16 3273.0 17 3273.0 18 5550.5 19 7828.0 20 7828.0 21 7828.0 22 12208.0 23 16588.0 24 16588.0 25 16588.0 26 23336.5 27 30085.0 28 30085.0 29 30085.0 30 39437.5 31 48790.0 32 48790.0 33 48790.0 34 55662.0 35 62534.0 36 62534.0 37 62534.0 38 67358.5 39 72183.0 40 72183.0 41 72183.0 42 76073.5 43 79964.0 44 79964.0 45 79964.0 46 83628.0 47 87292.0 48 87292.0 49 87292.0 50 83018.5 51 78745.0 52 78745.0 53 78745.0 54 67681.5 55 56618.0 56 56618.0 57 56618.0 58 47452.0 59 38286.0 60 38286.0 61 38286.0 62 30605.0 63 22924.0 64 22924.0 65 22924.0 66 17364.0 67 11804.0 68 11804.0 69 11804.0 70 8435.0 71 5066.0 72 5066.0 73 5066.0 74 3490.5 75 1915.0 76 1915.0 77 1915.0 78 1315.0 79 715.0 80 715.0 81 715.0 82 462.5 83 210.0 84 210.0 85 210.0 86 134.5 87 59.0 88 59.0 89 59.0 90 36.0 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11432320028533031 2 0.12053295628968672 3 0.018470043500136934 4 0.0 5 0.006368980517288597 6 0.34472107049824535 7 0.027864289763137613 8 0.011464164931119476 9 0.01130494041818726 10 0.004776735387966448 11 0.005891306978491952 12 0.0 13 4.7767353879664485E-4 14 1.5922451293221495E-4 15 0.0035029392845087283 16 4.7767353879664485E-4 17 0.0 18 3.184490258644299E-4 19 1.5922451293221495E-4 20 3.184490258644299E-4 21 0.013056410060441625 22 0.0 23 0.0 24 0.0 25 0.005413633439695308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628044.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.80399096339345 #Duplication Level Percentage of deduplicated Percentage of total 1 92.66853660102805 77.6599320390345 2 5.6764879088757585 9.514246828384726 3 0.8496870674060835 2.136215019558351 4 0.2806831115031704 0.940894597599554 5 0.1406891450598275 0.5895155920620666 6 0.0798416078176127 0.4014627228030011 7 0.05516836991992937 0.32363307019644355 8 0.03940729312441735 0.26419907495123746 9 0.025383874223579114 0.1914542969443866 >10 0.1521754477926159 2.4685165769759747 >50 0.015969842202238845 0.9551566238620364 >100 0.013117993360377727 2.216684081117143 >500 0.0019011584576125145 1.1716735952475827 >1k 9.505792288062573E-4 1.1664158812629255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2397 0.3816611574985192 No Hit GGTATCAACGCAGAGTACTTTTTTT 1476 0.23501538108794928 No Hit TATCAACGCAGAGTACTTTTTTTTT 1259 0.20046366178165861 No Hit GTACATGGGAAGCAGTGGTATCAAC 1153 0.18358586341084382 No Hit GTCCTACAGTGGACATTTCTAAATT 1036 0.16495659539777469 No Hit GTCCTACAGTGTGCATTTCTCATTT 946 0.15062638923387534 No Hit CCCATGTACTCTGCGTTGATACCAC 894 0.14234671456140016 No Hit CTGTAGGACGTGGAATATGGCAAGA 849 0.1351816114794505 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 847 0.13486316245358607 No Hit CTGTAGGACCTGGAATATGGCGAGA 752 0.11973683372502565 No Hit GTCCTAAAGTGTGTATTTCTCATTT 725 0.11543777187585584 No Hit CACACACACACACACACACACACAC 663 0.10556585207405851 No Hit CTTTAGGACGTGAAATATGGCGAGG 641 0.10206291278954976 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 35 0.0021346223 16.323868 3 CCTCGAA 35 0.0021817898 16.270555 17 GTGCGCC 35 0.0021876015 16.264078 11 TTGGACT 40 0.005201455 14.279962 4 TAAGATA 40 0.005201455 14.279962 4 GCCTCGA 40 0.005307148 14.236736 16 TGCGCCA 40 0.005321189 14.231069 12 TAGGACC 270 0.0 14.103666 4 GATATAC 175 0.0 13.617371 1 GTTACAA 200 0.0 13.287622 15 GCCCTGA 75 1.4248562E-5 12.709546 1 GTAGACT 85 3.8414237E-6 12.319966 4 CCAACGA 85 3.973899E-6 12.282675 19 CGCCACC 55 0.00309561 12.074846 14 GTCCTAT 95 9.950054E-7 12.040624 1 CTAGGAC 80 2.7922762E-5 11.902821 3 GTAGGAC 555 0.0 11.838482 3 ACTGTTC 235 0.0 11.706899 8 AGGACCT 415 0.0 11.699246 5 TTATACT 90 7.2686E-6 11.635524 4 >>END_MODULE