FastQCFastQC Report
Thu 26 May 2016
SRR1926805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793758
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10030.1263609311654182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC456.6916767E-414.7967638
GTGTTAC706.871529E-613.6423541
TAGGACC1750.012.52393154
TAGATTG701.0677446E-412.2408165
GTAGGAC3500.011.9923293
GATTAGA1052.535653E-711.8233741
GTACAAT751.9734375E-411.4595781
TGTAGGA4050.011.3058982
CTGTAGG3900.011.2636881
TAGTGTA600.005777264511.1074077
ATAGTGT951.3254781E-511.0238916
GGCGAGG2000.010.90043319
CTGCACT1407.930794E-910.8903764
TATGGCG3400.010.87255516
TCTTGGA1158.337811E-710.7836152
GTCTTGG1252.0784864E-710.6956061
ACTGCCC1201.4948382E-610.304898
TGGCGAG3450.010.16549518
TAGGACA2250.010.16435054
TGCACTG1502.354136E-810.1553435
Produced by FastQC (version 0.11.5)