FastQCFastQC Report
Thu 26 May 2016
SRR1926805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793758
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17810.22437569133161492No Hit
GGTATCAACGCAGAGTACTTTTTTT12450.15684881286235855No Hit
TATCAACGCAGAGTACTTTTTTTTT10750.13543170588516903No Hit
GTACATGGGAAGCAGTGGTATCAAC9050.1140145989079795No Hit
CCCATGTACTCTGCGTTGATACCAC8410.10595168804597875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC250.005951551319.0428626
GAGGCGT456.6426717E-414.8111156
TAGTACA801.2507553E-714.2821464
TCGCTGC400.005316660314.23355517
TCGAACT400.005316660314.23355519
GGTGATC500.001512949813.2829798
TAGGACC1950.013.1835214
GTATAGT603.9706245E-412.7105091
ATAGCAC604.0046865E-412.697653
AGGCGTG751.4921205E-512.6536447
GTCCTAC4400.012.1327591
AGGACCG550.003014841812.1181865
TATACTG1501.2732926E-1112.0604795
GGGTGAC951.0506028E-611.9876637
TTAATCC1209.651558E-911.901794
CTCTTAT1052.594188E-711.8026151
TCTTATA1052.5984446E-711.8011222
GTCATAG657.786736E-411.7327781
TAAGCTG657.8656746E-411.7186845
GGCGTGC658.102409E-411.6773438
Produced by FastQC (version 0.11.5)