FastQCFastQC Report
Thu 26 May 2016
SRR1926804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889219
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17700.1990510774061283No Hit
CTGTAGGACGTGGAATATGGCAAGA13410.15080649423820228No Hit
GTCCTAAAGTGTGTATTTCTCATTT11930.1341626753364469No Hit
GTCCTACAGTGTGCATTTCTCATTT11840.13315055121404287No Hit
GTATCAACGCAGAGTACTTTTTTTT10200.11470740053912479No Hit
CTTTAGGACGTGAAATATGGCGAGG9790.11009661287039525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCT853.845562E-612.3202394
TAGGACC3350.012.2199394
GGCTTAA550.00297136812.1419651
AAGTACC550.003018289612.1165994
TCTCGGA550.003106182412.07025719
GGACGTG5900.011.9338336
GACGTGG3750.011.9252337
TCTGTAC1302.4701876E-911.7323883
AGGACGT6200.011.663325
GATATAC2050.011.6342861
GTCCTAG1501.6370905E-1011.4481381
CCTAGAG1751.8189894E-1211.4390773
GTCCTAC9750.011.3502911
AAGGTGT1354.600224E-911.2768156
GCGATGA600.00594750111.06440213
GGCGAGG2400.011.06440219
TGGCGAG6200.011.01341418
CTGTAGG8700.010.965651
ACGTGAA2700.010.91579158
CCCTAGT700.001438816410.9029891
Produced by FastQC (version 0.11.5)