##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926804_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 889219 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.348402362072786 31.0 27.0 31.0 16.0 33.0 2 28.01258969950035 31.0 28.0 31.0 16.0 34.0 3 27.833284039139965 31.0 28.0 31.0 16.0 34.0 4 31.520990892007482 35.0 32.0 37.0 18.0 37.0 5 31.339865657391485 35.0 32.0 37.0 17.0 37.0 6 31.01483998879916 35.0 32.0 37.0 11.0 37.0 7 31.06362212233432 35.0 32.0 37.0 13.0 37.0 8 30.736228083295565 35.0 31.0 37.0 11.0 37.0 9 32.882643083424895 37.0 32.0 39.0 17.0 39.0 10 33.0004172200549 37.0 33.0 39.0 17.0 39.0 11 33.1827457577942 37.0 33.0 39.0 17.0 39.0 12 33.023752303988104 37.0 33.0 39.0 17.0 39.0 13 33.15938705763147 38.0 33.0 39.0 17.0 39.0 14 34.15674653825435 39.0 34.0 40.0 17.0 41.0 15 33.695043628172584 39.0 33.0 40.0 10.0 41.0 16 33.785106930913535 39.0 33.0 40.0 11.0 41.0 17 34.02411104576038 39.0 33.0 40.0 16.0 41.0 18 34.069084218848225 39.0 34.0 41.0 13.0 41.0 19 33.428183608312466 39.0 33.0 40.0 10.0 41.0 20 33.695044752754946 39.0 33.0 40.0 10.0 41.0 21 33.694736617188795 39.0 33.0 40.0 8.0 41.0 22 33.71786027963865 39.0 34.0 41.0 2.0 41.0 23 33.71174929910404 39.0 34.0 41.0 2.0 41.0 24 32.367650713716195 38.0 32.0 40.0 2.0 41.0 25 32.94711314085731 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.8716327902953438 1101 2 -0.9182041420077098 1101 3 -1.6285226391272971 1101 4 -1.086912353108179 1101 5 -0.9983827659258857 1101 6 -3.0637718911753424 1101 7 -1.4073025991728159 1101 8 -0.9359568339800042 1101 9 -1.0085394095200932 1101 10 -9.666303295664076 1101 11 -4.740933878145885 1101 12 -2.3756195405935436 1101 13 -1.4734222222305142 1101 14 -1.2304820417998386 1101 15 -2.9593937154228804 1101 16 -1.1218665831568657 1101 17 -0.8955281401355606 1101 18 -0.6892608504006503 1101 19 -1.3685658233200364 1101 20 -0.6628518039552702 1101 21 -0.8860817099297407 1101 22 -0.5102019939604574 1101 23 -0.6762829272989208 1101 24 -1.0246689147010244 1101 25 -0.8417180957747092 1102 1 -0.14960208107158834 1102 2 0.11668708471316336 1102 3 -0.08197353549968867 1102 4 0.07701209158042843 1102 5 0.014785850968767278 1102 6 -1.0591824462120591 1102 7 0.02417847537533646 1102 8 0.45740781287307897 1102 9 0.21785184466877894 1102 10 0.10941819713015377 1102 11 0.21514707920547238 1102 12 0.18089357995435051 1102 13 0.030594972737347348 1102 14 -0.2530904946189665 1102 15 0.4366696624086117 1102 16 0.15728383160993076 1102 17 0.06650192816820066 1102 18 -0.2258779831077078 1102 19 0.23462000685447748 1102 20 0.24903771974961586 1102 21 0.15262070176063958 1102 22 -0.04047630771826505 1102 23 -0.3359132080218785 1102 24 -3.576059253289902 1102 25 -2.1443055813540113 1103 1 -0.7621010061239808 1103 2 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-1.8631098994722848 2214 25 -1.1586365154454228 2215 1 0.6458938398382692 2215 2 0.02239680620976614 2215 3 0.4004924051023302 2215 4 0.5057184372782935 2215 5 0.9370912401903269 2215 6 1.305929276650943 2215 7 0.6940273681937263 2215 8 -0.3836222076228424 2215 9 0.14815968536322544 2215 10 -0.06706474302979615 2215 11 -0.34488888912632376 2215 12 -0.40967327291708955 2215 13 -0.02517704028358736 2215 14 -0.025671172190001812 2215 15 -0.1685115858331585 2215 16 0.2570879147188805 2215 17 -0.27521329909539105 2215 18 -1.0620683540436886 2215 19 0.3606729345561206 2215 20 -3.308926553047076 2215 21 -1.7846778554872778 2215 22 -0.906445041391855 2215 23 -0.7250945768278925 2215 24 -0.6255237471210329 2215 25 -0.443509857365747 2216 1 0.11597820414111837 2216 2 -1.0357139365375083 2216 3 -0.36500987246431293 2216 4 -0.4921387633362464 2216 5 -0.5139127758739335 2216 6 -1.8573160985860255 2216 7 -9.485775512231779 2216 8 -10.982104728309618 2216 9 -6.131523701427785 2216 10 -3.4602005974092833 2216 11 -2.6099266688806892 2216 12 -2.0456022236192233 2216 13 -1.6718765901609522 2216 14 -1.9668587412582212 2216 15 -2.9147349580693067 2216 16 -1.9911111455041102 2216 17 -2.2695809262033784 2216 18 -1.9232733530104724 2216 19 -1.4002337795486213 2216 20 -2.4255478480705683 2216 21 -5.343114734996366 2216 22 -3.772514239886057 2216 23 -3.5226561879255236 2216 24 -3.880241050338377 2216 25 -3.184895085077489 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54424.0 3 10.0 4 451.0 5 2559.0 6 3108.0 7 2551.0 8 2074.0 9 1926.0 10 1775.0 11 1760.0 12 1806.0 13 1736.0 14 1728.0 15 1720.0 16 1914.0 17 2140.0 18 2331.0 19 2979.0 20 3779.0 21 5142.0 22 6177.0 23 7534.0 24 8595.0 25 9884.0 26 11110.0 27 12422.0 28 13673.0 29 16189.0 30 19400.0 31 23521.0 32 29859.0 33 38871.0 34 52114.0 35 72140.0 36 104181.0 37 165349.0 38 202287.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.486612280877765 18.226979694945097 12.390220477748757 25.896187546428383 2 14.347397642872025 19.732866337442072 41.96318465670734 23.956551362978562 3 18.998783674204883 23.95362194792324 29.604131002793043 27.443463375078835 4 11.602871486463522 16.36812555494661 38.63328678065134 33.395716177938525 5 13.01254528868337 37.82787544473178 36.24939399495399 12.910185271630864 6 32.43467599518576 37.14801525257865 18.123010472087557 12.294298280148027 7 28.024097825805015 30.907311109832992 22.877145972963252 18.19144509139874 8 24.56754272697029 37.543234449976595 20.730770748870857 17.158452074182254 9 25.99705077459347 15.51773978203627 20.072167163265835 38.41304228010443 10 13.986605600987312 29.63226715897214 35.22672939736455 21.154397842676 11 34.58965355120698 22.57653434231867 24.450948060800066 18.382864045674282 12 23.054228305078645 24.807323723140048 32.155229816794446 19.98321815498686 13 28.92775316423662 22.187054664556612 25.579561725249896 23.305630445956872 14 20.254043025120858 22.36840625288225 27.80919665895067 29.568354063046222 15 22.992004121100056 31.546998424219115 22.935316500259255 22.525680954421578 16 21.567685702491328 28.436194874713593 28.401213439619905 21.594905983175167 17 21.310346922041244 29.040646894933563 28.10267623646444 21.546329946560753 18 21.206627000641102 28.025846652194943 29.828813730893383 20.938712616270568 19 23.620795739975637 27.150343809792705 27.26249115588256 21.9663692943491 20 23.060423368501564 27.834116932490495 28.186511596517672 20.91894810249027 21 22.485763485463178 27.27370887213512 27.206223912845424 23.034303729556274 22 22.370194070578073 28.632890092659874 27.49381930087801 21.503096535884048 23 21.672273756457713 28.32665769082383 27.824550392383806 22.17651816033465 24 23.024414055909606 27.26971086901262 28.354317618926572 21.351557456151205 25 22.20687560806897 27.78654459359904 28.075830769836408 21.93074902849559 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 284.0 1 284.0 2 384.0 3 484.0 4 484.0 5 484.0 6 684.0 7 884.0 8 884.0 9 884.0 10 1297.5 11 1711.0 12 1711.0 13 1711.0 14 2950.5 15 4190.0 16 4190.0 17 4190.0 18 7167.5 19 10145.0 20 10145.0 21 10145.0 22 16112.0 23 22079.0 24 22079.0 25 22079.0 26 31724.5 27 41370.0 28 41370.0 29 41370.0 30 54307.0 31 67244.0 32 67244.0 33 67244.0 34 77410.0 35 87576.0 36 87576.0 37 87576.0 38 94971.5 39 102367.0 40 102367.0 41 102367.0 42 107520.5 43 112674.0 44 112674.0 45 112674.0 46 115535.0 47 118396.0 48 118396.0 49 118396.0 50 113053.0 51 107710.0 52 107710.0 53 107710.0 54 95267.5 55 82825.0 56 82825.0 57 82825.0 58 70745.0 59 58665.0 60 58665.0 61 58665.0 62 47564.0 63 36463.0 64 36463.0 65 36463.0 66 28046.0 67 19629.0 68 19629.0 69 19629.0 70 14299.0 71 8969.0 72 8969.0 73 8969.0 74 6247.0 75 3525.0 76 3525.0 77 3525.0 78 2455.0 79 1385.0 80 1385.0 81 1385.0 82 902.5 83 420.0 84 420.0 85 420.0 86 296.0 87 172.0 88 172.0 89 172.0 90 105.0 91 38.0 92 38.0 93 38.0 94 22.5 95 7.0 96 7.0 97 7.0 98 7.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23616229522760984 2 0.1432717924380833 3 0.14608324833365008 4 0.06972410621005623 5 0.02249164716453427 6 0.021254606570484884 7 0.2811455895566784 8 0.27934625778351563 9 0.01934281656149947 10 0.03823580017970826 11 0.01585661125099666 12 0.018330692439095433 13 0.015969069486819334 14 0.0176559430241594 15 0.015294320071883305 16 0.01979264950479016 17 0.01934281656149947 18 0.014507112421124606 19 0.023616229522760986 20 0.017880859495804747 21 0.01484448712859262 22 0.01810577596745009 23 0.018668067146563445 24 0.01585661125099666 25 0.015069403600237962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 889219.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.93876093680805 #Duplication Level Percentage of deduplicated Percentage of total 1 93.2690091424711 80.15423079507788 2 5.103187299493788 8.771231866939035 3 0.820917712437855 2.1164595311396446 4 0.28717451228152685 0.9871768703242632 5 0.14856167185969796 0.6383603001161553 6 0.07880435471049102 0.40634091601465866 7 0.05459044514403213 0.32840046502668374 8 0.04061887865749772 0.2792588881974329 9 0.029261462953987634 0.2263224482517608 >10 0.1429917567670845 2.2293978209230136 >50 0.013229713898060283 0.7720573182152697 >100 0.010081851835534906 1.782803511948202 >500 9.165318688626967E-4 0.5756641099620118 >1k 6.54665620616212E-4 0.7322951578641073 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1770 0.1990510774061283 No Hit CTGTAGGACGTGGAATATGGCAAGA 1341 0.15080649423820228 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1193 0.1341626753364469 No Hit GTCCTACAGTGTGCATTTCTCATTT 1184 0.13315055121404287 No Hit GTATCAACGCAGAGTACTTTTTTTT 1020 0.11470740053912479 No Hit CTTTAGGACGTGAAATATGGCGAGG 979 0.11009661287039525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTCT 85 3.845562E-6 12.320239 4 TAGGACC 335 0.0 12.219939 4 GGCTTAA 55 0.002971368 12.141965 1 AAGTACC 55 0.0030182896 12.116599 4 TCTCGGA 55 0.0031061824 12.070257 19 GGACGTG 590 0.0 11.933833 6 GACGTGG 375 0.0 11.925233 7 TCTGTAC 130 2.4701876E-9 11.732388 3 AGGACGT 620 0.0 11.66332 5 GATATAC 205 0.0 11.634286 1 GTCCTAG 150 1.6370905E-10 11.448138 1 CCTAGAG 175 1.8189894E-12 11.439077 3 GTCCTAC 975 0.0 11.350291 1 AAGGTGT 135 4.600224E-9 11.276815 6 GCGATGA 60 0.005947501 11.064402 13 GGCGAGG 240 0.0 11.064402 19 TGGCGAG 620 0.0 11.013414 18 CTGTAGG 870 0.0 10.96565 1 ACGTGAA 270 0.0 10.9157915 8 CCCTAGT 70 0.0014388164 10.902989 1 >>END_MODULE