FastQCFastQC Report
Thu 26 May 2016
SRR1926804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889219
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18340.2062484044987793No Hit
GTATCAACGCAGAGTACTTTTTTTT18150.2041116980181485No Hit
CTGTAGGACGTGGAATATGGCAAGA16280.18308200791930898No Hit
GTCCTAAAGTGTGTATTTCTCATTT13630.15328057542630105No Hit
CTTTAGGACGTGAAATATGGCGAGG12810.144059000088842No Hit
GTCCTACAGTGTGCATTTCTCATTT12520.14079771124998453No Hit
GGTATCAACGCAGAGTACTTTTTTT11040.12415389234822918No Hit
GTACATGGGAAGCAGTGGTATCAAC10640.11965556291532232No Hit
CTGTAGGACCTGGAATATGGCGAGA9680.10885957227634588No Hit
TATCAACGCAGAGTACTTTTTTTTT9480.10661040755989244No Hit
CCCATGTACTCTGCGTTGATACCAC9190.10334911872103497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA502.1960841E-718.9789719
TCCAACG901.364242E-1015.81580918
GCCGGTT602.592757E-514.23422711
TAGGACC4600.014.0706594
CCCTTAG500.001463224213.3422971
CTAGGAC1207.1486284E-1012.6926143
GTAGGAC9800.012.6278553
TGTAGGA10550.012.3750272
GTCCATA550.002994584212.1293611
TCGAACT550.003092211212.07752619
GTCCTAC9600.012.0120381
TATACTG2000.011.89798455
CTGTAGG11250.011.8598191
GGACCTG6950.011.7781486
AGGACCT7300.011.7349985
CCGGTTT658.092329E-411.67936512
CCAACGT658.092329E-411.67936519
CCTATAC907.282535E-611.6348973
CGGTTTC752.0933332E-411.38738213
ACTGTTC2250.011.3873828
Produced by FastQC (version 0.11.5)