##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926804_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 889219 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.951290964318126 31.0 30.0 33.0 26.0 34.0 2 30.29946503617219 31.0 30.0 34.0 26.0 34.0 3 30.35840664673157 31.0 30.0 34.0 26.0 34.0 4 33.98616763699381 37.0 35.0 37.0 30.0 37.0 5 33.98909717403699 37.0 35.0 37.0 30.0 37.0 6 33.92073493706275 37.0 35.0 37.0 30.0 37.0 7 33.919561997663116 37.0 35.0 37.0 30.0 37.0 8 33.9364903359015 37.0 35.0 37.0 30.0 37.0 9 35.51147130234509 39.0 35.0 39.0 30.0 39.0 10 35.40385101982751 39.0 35.0 39.0 30.0 39.0 11 35.437661588427595 39.0 35.0 39.0 30.0 39.0 12 35.34197087556608 39.0 35.0 39.0 30.0 39.0 13 35.35186832490084 39.0 35.0 39.0 30.0 39.0 14 36.468835011397644 40.0 37.0 41.0 30.0 41.0 15 36.446314125091796 40.0 37.0 41.0 30.0 41.0 16 36.43799558938799 40.0 37.0 41.0 30.0 41.0 17 36.409025223257714 40.0 37.0 41.0 30.0 41.0 18 36.390513473058945 40.0 37.0 41.0 30.0 41.0 19 36.45045033900535 40.0 37.0 41.0 30.0 41.0 20 36.408534905349526 40.0 37.0 41.0 30.0 41.0 21 36.35865517943274 40.0 37.0 41.0 30.0 41.0 22 36.27743221860981 40.0 37.0 41.0 29.0 41.0 23 36.206465448893915 40.0 37.0 41.0 29.0 41.0 24 36.13832250547953 40.0 37.0 41.0 29.0 41.0 25 36.028923133671235 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2411507332327929 1101 2 -0.19130269392570298 1101 3 0.18246814694470714 1101 4 0.16753170408662754 1101 5 0.03745218398756123 1101 6 -0.015534400067998888 1101 7 0.028773866283557936 1101 8 -0.16280228704508204 1101 9 0.11607451640254851 1101 10 -0.048987633778565964 1101 11 -0.0659137956796485 1101 12 -0.11633087467944847 1101 13 0.04067383627039334 1101 14 0.1710755171877949 1101 15 0.037472915273916385 1101 16 0.1059548560924668 1101 17 0.05792410297559769 1101 18 0.15308958472530776 1101 19 0.14146842705120832 1101 20 0.18483545522680345 1101 21 -0.003395404639277899 1101 22 -0.09287821979989275 1101 23 0.00409269860156769 1101 24 0.15446665263967674 1101 25 0.03096300131879559 1102 1 -0.36986806920305426 1102 2 -0.3267748688419232 1102 3 -0.11925867464147899 1102 4 -0.16097870628528455 1102 5 -0.46694269597728066 1102 6 -0.31518967667075515 1102 7 -0.18558869300154157 1102 8 -0.2053067277050431 1102 9 -0.22565257986363463 1102 10 -0.24628112020021575 1102 11 -0.20597312655917221 1102 12 -0.06477115009192147 1102 13 -0.3182848518579391 1102 14 -0.19328292189734242 1102 15 -0.3211630238569967 1102 16 -0.16095627577224292 1102 17 -0.11997010860124391 1102 18 -0.1947079394923108 1102 19 -0.1062585959669704 1102 20 -0.06749474675455502 1102 21 -0.16885119031563534 1102 22 -0.1789057061116779 1102 23 -0.16956228500919224 1102 24 -0.35577204427841735 1102 25 -0.20756910877295098 1103 1 -0.768105041969541 1103 2 -0.7438850928383758 1103 3 -0.4687467732741446 1103 4 -0.587228813780051 1103 5 -0.6918986797014455 1103 6 -0.8379372653935349 1103 7 -0.9157468573983749 1103 8 -0.8613172689807698 1103 9 -0.9654558531356798 1103 10 -0.7818664122012535 1103 11 -0.81866158184814 1103 12 -0.7230888683968146 1103 13 -0.578469131824086 1103 14 -0.7624020532491471 1103 15 -0.7527782514516943 1103 16 -0.6969794674840557 1103 17 -1.02918851985131 1103 18 -0.7919720280951523 1103 19 -0.9212799138753738 1103 20 -0.8084346356841792 1103 21 -0.7927468230863752 1103 22 -0.6896817730254696 1103 23 -0.7692591497830463 1103 24 -0.6547381709819291 1103 25 -0.6045834700730239 1104 1 -0.42911077894242666 1104 2 -0.38797093359592694 1104 3 -0.06341197167826707 1104 4 -0.09604239132066539 1104 5 -0.4239213257072336 1104 6 -0.3447096835556209 1104 7 -0.40821114854431073 1104 8 -0.37489984102455054 1104 9 -0.44994189835222187 1104 10 -0.5703885785059981 1104 11 -0.5103613244447942 1104 12 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0.050121565269122925 1107 9 -0.009361554875482625 1107 10 0.05206207610511626 1107 11 -0.019167851478833597 1107 12 -0.13482971331738014 1107 13 -0.0371396642273325 1107 14 0.07755224062219668 1107 15 0.024074653281395797 1107 16 -0.014416510727549792 1107 17 0.08993778821508869 1107 18 0.0208938481347829 1107 19 -0.02343377639790134 1107 20 -0.020609046015266586 1107 21 -0.012264802973014355 1107 22 -0.043659336680448746 1107 23 -0.07435753322244665 1107 24 -0.10899214366907728 1107 25 -0.11079491522456664 1108 1 0.6385701452770753 1108 2 0.5354145704612883 1108 3 0.678645516975287 1108 4 0.5952297858214095 1108 5 0.6347666126781348 1108 6 0.6402904539770375 1108 7 0.5307733102646566 1108 8 0.5261157001664856 1108 9 0.4058231958576499 1108 10 0.5404374426740333 1108 11 0.521852676380405 1108 12 0.4551292309641468 1108 13 0.47202162609524834 1108 14 0.6196871379829005 1108 15 0.5487139494690751 1108 16 0.6510263045217144 1108 17 0.7656210726725732 1108 18 0.47914018244563295 1108 19 0.7285193027809882 1108 20 0.687261921726666 1108 21 0.6938700943876839 1108 22 0.5303817190380258 1108 23 0.46330815592711616 1108 24 0.44393454254792886 1108 25 0.486794527590682 1109 1 0.4630110901589646 1109 2 0.38398149959514427 1109 3 0.5376290531241779 1109 4 0.6026170442322893 1109 5 0.5151961044462112 1109 6 0.4995946773127571 1109 7 0.32963372544002567 1109 8 0.35468549257879545 1109 9 0.38837437696001587 1109 10 0.46476671969622174 1109 11 0.4473387178979422 1109 12 0.3752494886592075 1109 13 0.6144640026163657 1109 14 0.5566549261002933 1109 15 0.42288431453707886 1109 16 0.49163331955392664 1109 17 0.5052517097520237 1109 18 0.3976054651943741 1109 19 0.4767855876771918 1109 20 0.438208235255658 1109 21 0.5294720986826036 1109 22 0.574035262345113 1109 23 0.39083285170377735 1109 24 0.3962495031778488 1109 25 0.31730562279469154 1110 1 0.08429415525724337 1110 2 -0.20329031086428273 1110 3 0.01925072457556709 1110 4 0.16988016007769602 1110 5 0.2517508107460742 1110 6 0.2594641472788126 1110 7 0.03689473563731127 1110 8 0.11595486592683102 1110 9 0.0908946564168005 1110 10 0.20812498665713974 1110 11 0.12071340186181345 1110 12 0.040848890614839206 1110 13 0.15916998512538072 1110 14 0.04037116331716817 1110 15 0.16908168026045445 1110 16 0.042718710637764445 1110 17 0.11275638071428062 1110 18 0.06785542779141451 1110 19 0.17427095014839722 1110 20 0.11657904795019647 1110 21 0.26438560843029535 1110 22 0.21265617364745282 1110 23 0.11587651367774043 1110 24 0.2234644510630588 1110 25 0.2338453880602671 1111 1 0.29185592417479356 1111 2 0.22586930975184671 1111 3 0.19179405997457621 1111 4 0.44811600408075947 1111 5 0.21474722907665722 1111 6 0.3554269791434166 1111 7 0.06589820527014467 1111 8 -0.010345371100555667 1111 9 0.2024235706072801 1111 10 0.1696684305915852 1111 11 0.14926522403175824 1111 12 0.02930562173754936 1111 13 0.2582768435146434 1111 14 0.2703367470802718 1111 15 0.22445575989745237 1111 16 0.10875377108500572 1111 17 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0.6506924965405219 2208 13 0.5882242348484752 2208 14 0.6314103717775126 2208 15 0.8115613307840448 2208 16 0.7814422483739278 2208 17 0.6849573634757249 2208 18 0.6157954165050867 2208 19 0.7177712394823033 2208 20 0.6727567240838184 2208 21 0.8402426031607817 2208 22 0.8198616899047408 2208 23 0.653551683703057 2208 24 0.6996199030723318 2208 25 0.7707630106735195 2209 1 0.1750717487851574 2209 2 0.13716442320033906 2209 3 0.09189245722006589 2209 4 0.09887793033348657 2209 5 0.04923876399693938 2209 6 -0.025909166416965945 2209 7 -0.01978261617032473 2209 8 -0.20105335539997782 2209 9 -0.07533604231238655 2209 10 -0.09854311888104661 2209 11 -0.04098991803022756 2209 12 -0.0377134943451054 2209 13 0.014265490222477695 2209 14 -0.02514511543682829 2209 15 -0.031722466526730386 2209 16 0.021488769342710157 2209 17 0.10130063285321 2209 18 0.13112473640858724 2209 19 -0.10711810804433242 2209 20 -0.09574063079640638 2209 21 -0.12527042527982957 2209 22 -0.18681914489285845 2209 23 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10 0.31651078541028 2211 11 0.429272602011892 2211 12 0.26723796285284607 2211 13 0.2588742995149147 2211 14 0.20426823812394446 2211 15 0.23092602794067574 2211 16 0.17337224630912118 2211 17 0.27877506677437935 2211 18 0.1293558511211046 2211 19 0.22836235958313722 2211 20 0.277661203685895 2211 21 0.1886914817450389 2211 22 0.18037889816355346 2211 23 0.21867217695545094 2211 24 0.18744371038996377 2211 25 0.30124612394919126 2212 1 0.1410772957842248 2212 2 0.2684349194816349 2212 3 0.4561922945220651 2212 4 0.5210081515997729 2212 5 0.3372576351691592 2212 6 0.30230973199631705 2212 7 0.2592601747515175 2212 8 0.32769120574199206 2212 9 0.23334263137708433 2212 10 0.18359198182857028 2212 11 0.2515027740304987 2212 12 0.28999800183291313 2212 13 0.4168794349530529 2212 14 0.32428589058164903 2212 15 0.4452895180446461 2212 16 0.24418147367688192 2212 17 0.332872127923622 2212 18 0.36109469640014424 2212 19 0.3354084856701718 2212 20 0.41015286861761524 2212 21 0.27532438784198376 2212 22 0.3394572561135618 2212 23 0.42661490323386175 2212 24 0.2520398786532638 2212 25 0.22330407210022685 2213 1 0.3276438108026234 2213 2 0.3052393454074007 2213 3 0.31044468015520366 2213 4 0.15497620397786704 2213 5 0.28890126463705457 2213 6 0.37879787124596476 2213 7 0.3375272090855006 2213 8 0.3277578961852541 2213 9 0.516030372223824 2213 10 0.40803576903386585 2213 11 0.4556784656538895 2213 12 0.3411147767990599 2213 13 0.3760576347170286 2213 14 0.3178517125993565 2213 15 0.30691554703469137 2213 16 0.4719452851227075 2213 17 0.38951406113459797 2213 18 0.49935268403051936 2213 19 0.4362139902413631 2213 20 0.5146756405044002 2213 21 0.43020279376641213 2213 22 0.22288381108823074 2213 23 0.39041504067417065 2213 24 0.41523719247819457 2213 25 0.5061686876008267 2214 1 0.29898765369458147 2214 2 0.347697398744117 2214 3 0.4698913082210794 2214 4 0.2037146343062588 2214 5 0.3821066800182038 2214 6 0.4108224405090297 2214 7 0.37120428669562955 2214 8 0.24299112104190357 2214 9 0.4119646099990675 2214 10 0.1777707760073639 2214 11 0.41176513429285677 2214 12 0.265930577765495 2214 13 0.31105192912554713 2214 14 0.23970060599636867 2214 15 0.14472741748254236 2214 16 0.040682870178279984 2214 17 0.2580924531439521 2214 18 0.21481695012239754 2214 19 0.2845058347675149 2214 20 0.18582084428558687 2214 21 0.17446268698027723 2214 22 0.26029417695048096 2214 23 0.2981633747823338 2214 24 0.25196147857486295 2214 25 0.20808745688361796 2215 1 0.2526201757834805 2215 2 0.1584017577706227 2215 3 0.05112873845179777 2215 4 0.2717032452478918 2215 5 0.2729118231723362 2215 6 0.28298477978545833 2215 7 0.04889167512127557 2215 8 0.17672216752463044 2215 9 -0.0030919963327065147 2215 10 0.018934086969331076 2215 11 -0.09025958719495009 2215 12 -0.10240829191565837 2215 13 -0.09570015547888744 2215 14 -0.12629943919831277 2215 15 -0.11681869238571352 2215 16 -0.29227082787608794 2215 17 -0.3909573287917354 2215 18 -0.2266243080973851 2215 19 -0.3352171218681903 2215 20 -0.27840982934105796 2215 21 -0.19152831927918612 2215 22 -0.2613766702237186 2215 23 -0.24088708507349565 2215 24 -0.2236627728883107 2215 25 -0.23005233024946392 2216 1 -0.38706159380044625 2216 2 -0.10176104107577189 2216 3 -2.2450492621411797 2216 4 -1.8787850614464503 2216 5 -0.9432350911067005 2216 6 -0.5661926907229713 2216 7 -0.6751427174827 2216 8 -0.5323323952454686 2216 9 -0.543177169239101 2216 10 -0.4807298933366795 2216 11 -0.5134224848706666 2216 12 -0.4128410745001361 2216 13 -0.5674682120451919 2216 14 -0.7482593144936729 2216 15 -0.6878473259355715 2216 16 -0.653280863544488 2216 17 -0.5254092303577309 2216 18 -0.5455213728665314 2216 19 -0.6747290847083676 2216 20 -0.42520102095314627 2216 21 -0.474926790120044 2216 22 -0.5972166745362699 2216 23 -0.5009887894300675 2216 24 -0.5767026729808507 2216 25 -0.5894298852120414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19503.0 3 17.0 4 307.0 5 2053.0 6 2913.0 7 2078.0 8 1641.0 9 1402.0 10 1329.0 11 1250.0 12 1081.0 13 1075.0 14 1005.0 15 1107.0 16 1073.0 17 1151.0 18 1219.0 19 1614.0 20 2006.0 21 2492.0 22 3248.0 23 3942.0 24 4764.0 25 5494.0 26 6833.0 27 7641.0 28 8924.0 29 10765.0 30 13843.0 31 17809.0 32 22891.0 33 30834.0 34 41501.0 35 58354.0 36 86138.0 37 150992.0 38 368930.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.066646251120225 19.112779381499013 10.3689209332907 28.45165343409006 2 14.282191595730307 20.51254334999775 40.310431022834756 24.894834031437192 3 18.552066227251256 24.322317953793895 29.109171484489238 28.016444334465618 4 11.933505694322772 17.038997142436227 37.976583946137005 33.050913217104 5 12.714175659185864 38.533343080305684 35.70518407719602 13.047297183312434 6 32.05640884451686 38.025983814841055 17.507593845487246 12.410013495154832 7 28.504179335153324 31.592534226004677 21.475404857274945 18.427881581567057 8 25.05218619675096 38.45695313589555 19.543280802227812 16.947579865125675 9 26.118125521581963 15.623952618425818 19.56851815611356 38.689403703878654 10 14.087264768961031 29.92378362511935 34.58235068529509 21.40660092062453 11 34.68182544091879 22.70667119525488 24.203579534080248 18.407923829746075 12 23.185454169229782 24.941156095756153 31.838572586893864 20.0348171481202 13 29.170513701441614 22.195919312920527 25.411684264625013 23.22188272101285 14 20.488114275689426 22.523723091525362 27.397331138146104 29.590831494639108 15 22.6877676611365 31.592561269658475 23.037751466513118 22.681919602691906 16 21.740274578954942 28.667162984021882 27.872755326040018 21.719807110983158 17 21.30813669073648 29.23239381974519 27.832738616696222 21.626730872822105 18 21.220940693824673 28.075068374838057 29.78432686771268 20.919664063624584 19 23.227063810071108 27.283553958145202 27.423002484208013 22.066379747575677 20 23.14136208085127 27.830902157300898 28.175813893858955 20.851921867988885 21 22.332821576996835 27.191582451818984 27.227461632334006 23.24813433885018 22 22.481975756253522 28.634790754583516 27.26617402462161 21.617059464541356 23 21.734490304964588 28.348953799855604 27.726046931123747 22.190508964056058 24 22.648413945271077 27.263924859905153 28.526156098778817 21.561505096044957 25 22.250076195424676 27.710842018487092 28.06679555221158 21.972286233876652 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 278.0 1 278.0 2 292.0 3 306.0 4 306.0 5 306.0 6 511.5 7 717.0 8 717.0 9 717.0 10 1142.0 11 1567.0 12 1567.0 13 1567.0 14 2642.5 15 3718.0 16 3718.0 17 3718.0 18 6819.5 19 9921.0 20 9921.0 21 9921.0 22 15559.0 23 21197.0 24 21197.0 25 21197.0 26 30805.5 27 40414.0 28 40414.0 29 40414.0 30 53406.5 31 66399.0 32 66399.0 33 66399.0 34 76231.0 35 86063.0 36 86063.0 37 86063.0 38 93724.0 39 101385.0 40 101385.0 41 101385.0 42 106663.0 43 111941.0 44 111941.0 45 111941.0 46 115929.0 47 119917.0 48 119917.0 49 119917.0 50 114984.0 51 110051.0 52 110051.0 53 110051.0 54 96968.5 55 83886.0 56 83886.0 57 83886.0 58 71866.0 59 59846.0 60 59846.0 61 59846.0 62 48430.5 63 37015.0 64 37015.0 65 37015.0 66 28606.0 67 20197.0 68 20197.0 69 20197.0 70 14650.5 71 9104.0 72 9104.0 73 9104.0 74 6358.5 75 3613.0 76 3613.0 77 3613.0 78 2400.5 79 1188.0 80 1188.0 81 1188.0 82 776.0 83 364.0 84 364.0 85 364.0 86 235.0 87 106.0 88 106.0 89 106.0 90 63.5 91 21.0 92 21.0 93 21.0 94 12.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11324544347343006 2 0.12359160116911581 3 0.017880859495804747 4 0.0 5 0.006072744734424253 6 0.3345632515724473 7 0.022716563636179615 8 0.010683532403153779 9 0.010908448874799121 10 0.004498329432906854 11 0.005960286498601582 12 2.2491647164534273E-4 13 6.747494149360281E-4 14 1.1245823582267137E-4 15 0.003936038253793497 16 3.3737470746801406E-4 17 0.0 18 3.3737470746801406E-4 19 2.2491647164534273E-4 20 6.747494149360281E-4 21 0.013944821242011247 22 0.0 23 1.1245823582267137E-4 24 0.0 25 0.006522577677714939 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 889219.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.29724641531963 #Duplication Level Percentage of deduplicated Percentage of total 1 90.90579474644433 74.81296590828597 2 6.901826891527965 11.36002696815913 3 1.149251125998257 2.837406093280863 4 0.37812818689928346 1.2447563429531345 5 0.186821066743781 0.7687429682692911 6 0.11380071170681957 0.5619291128144928 7 0.06460263088415533 0.3721633043065889 8 0.05654808148788393 0.37230011172175664 9 0.036010386105183534 0.2667200056928182 >10 0.17861754940830615 2.6589532314820787 >50 0.015327634046467135 0.8922938887718332 >100 0.011218774219992312 1.975581525110839 >500 9.57700112889323E-4 0.5992274133927191 >1k 0.001094514414730655 1.276933125758517 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1834 0.2062484044987793 No Hit GTATCAACGCAGAGTACTTTTTTTT 1815 0.2041116980181485 No Hit CTGTAGGACGTGGAATATGGCAAGA 1628 0.18308200791930898 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1363 0.15328057542630105 No Hit CTTTAGGACGTGAAATATGGCGAGG 1281 0.144059000088842 No Hit GTCCTACAGTGTGCATTTCTCATTT 1252 0.14079771124998453 No Hit GGTATCAACGCAGAGTACTTTTTTT 1104 0.12415389234822918 No Hit GTACATGGGAAGCAGTGGTATCAAC 1064 0.11965556291532232 No Hit CTGTAGGACCTGGAATATGGCGAGA 968 0.10885957227634588 No Hit TATCAACGCAGAGTACTTTTTTTTT 948 0.10661040755989244 No Hit CCCATGTACTCTGCGTTGATACCAC 919 0.10334911872103497 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 50 2.1960841E-7 18.97897 19 TCCAACG 90 1.364242E-10 15.815809 18 GCCGGTT 60 2.592757E-5 14.234227 11 TAGGACC 460 0.0 14.070659 4 CCCTTAG 50 0.0014632242 13.342297 1 CTAGGAC 120 7.1486284E-10 12.692614 3 GTAGGAC 980 0.0 12.627855 3 TGTAGGA 1055 0.0 12.375027 2 GTCCATA 55 0.0029945842 12.129361 1 TCGAACT 55 0.0030922112 12.077526 19 GTCCTAC 960 0.0 12.012038 1 TATACTG 200 0.0 11.8979845 5 CTGTAGG 1125 0.0 11.859819 1 GGACCTG 695 0.0 11.778148 6 AGGACCT 730 0.0 11.734998 5 CCGGTTT 65 8.092329E-4 11.679365 12 CCAACGT 65 8.092329E-4 11.679365 19 CCTATAC 90 7.282535E-6 11.634897 3 CGGTTTC 75 2.0933332E-4 11.387382 13 ACTGTTC 225 0.0 11.387382 8 >>END_MODULE