FastQCFastQC Report
Thu 26 May 2016
SRR1926803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924748
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23860.25801623793725426No Hit
CTGTAGGACGTGGAATATGGCAAGA18200.19681037428575135No Hit
GTCCTAAAGTGTGTATTTCTCATTT18140.1961615488760181No Hit
GTCCTACAGTGTGCATTTCTCATTT15500.16761323084775528No Hit
CTTTAGGACGTGAAATATGGCGAGG13090.14155207689013655No Hit
CTGTAGGACCTGGAATATGGCGAGA10970.11862691241289519No Hit
GTATCAACGCAGAGTACTTTTTTTT10150.10975963181320748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGC400.005234454814.2673768
TAGGACC3900.013.6776974
TCCAACG1051.4097168E-913.54388418
TAAGGCC751.4368188E-512.7007194
TGGGCCG604.0196825E-412.6924445
ACTATGC853.885103E-612.3091098
CCAACGA854.028898E-612.26916619
GGACCTA701.0694805E-412.2391416
ACTGTTC3150.012.0782028
GCCGGTT550.003113843312.06637111
CCGGTTT550.003113843312.06637112
CGTTTCT550.003113843312.06637114
AGGACCT7200.011.634745
GTTCTAT1406.2755134E-1011.5887661
GTATTAT1001.823093E-611.4524291
GTCCTAC11800.011.4039011
TGTAGAA4200.011.3504132
GCCTTAT855.0743147E-511.2278711
GTCTAGG855.0743147E-511.2278711
CCGTTTC1105.0911876E-711.20448713
Produced by FastQC (version 0.11.5)