##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926803_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 924748 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.17232802882515 31.0 27.0 31.0 16.0 33.0 2 27.80962813653017 31.0 28.0 31.0 16.0 34.0 3 27.63281131724535 31.0 28.0 31.0 10.0 34.0 4 31.2646299316138 35.0 32.0 37.0 17.0 37.0 5 31.07457707397042 35.0 32.0 37.0 16.0 37.0 6 30.758263872968637 35.0 32.0 37.0 11.0 37.0 7 30.799219895582365 35.0 32.0 37.0 10.0 37.0 8 30.46381608827486 35.0 30.0 37.0 10.0 37.0 9 32.5880520963549 37.0 32.0 39.0 13.0 39.0 10 32.70006964059398 37.0 33.0 39.0 11.0 39.0 11 32.89072158036568 37.0 33.0 39.0 13.0 39.0 12 32.721395450436226 37.0 33.0 39.0 10.0 39.0 13 32.857872631246565 38.0 33.0 39.0 10.0 39.0 14 33.84688044743 39.0 33.0 40.0 10.0 41.0 15 33.408571848763124 39.0 33.0 40.0 10.0 41.0 16 33.49056067166406 39.0 33.0 40.0 10.0 41.0 17 33.72367607175144 39.0 33.0 40.0 10.0 41.0 18 33.77621038380186 39.0 33.0 41.0 8.0 41.0 19 33.141802956048565 39.0 32.0 40.0 2.0 41.0 20 33.396028972217294 39.0 33.0 41.0 2.0 41.0 21 33.39834311617868 39.0 33.0 41.0 2.0 41.0 22 33.42198739548504 39.0 33.0 41.0 2.0 41.0 23 33.411586724167016 39.0 34.0 41.0 2.0 41.0 24 32.10099400052771 38.0 31.0 40.0 2.0 41.0 25 32.67275733497126 39.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6515919143580646 1101 2 -0.6089622812092976 1101 3 -1.263673113106229 1101 4 -0.8345645260242485 1101 5 -0.6925260752351825 1101 6 -2.8126356116207525 1101 7 -0.9823191512258092 1101 8 -0.5517444781717771 1101 9 -0.7452200817450638 1101 10 -9.910487045706283 1101 11 -4.855511696549954 1101 12 -2.2633195648953155 1101 13 -1.4931417062860355 1101 14 -1.267476426402446 1101 15 -2.360521699107359 1101 16 -0.9734164958392952 1101 17 -0.7611972794467903 1101 18 -0.6910478211292101 1101 19 -1.4129262069056985 1101 20 -0.8173951704307285 1101 21 -0.8312833356221887 1101 22 -0.5572202836475952 1101 23 -0.7017520616319004 1101 24 -0.8250281393949095 1101 25 -0.6072503063217809 1102 1 -0.650046800222082 1102 2 -0.3866139634656349 1102 3 -0.5445269112098963 1102 4 -0.45477866818753654 1102 5 -0.451375316850541 1102 6 -1.1833116896309015 1102 7 -0.4688041977177626 1102 8 -0.11814389337271436 1102 9 -0.14892324081083075 1102 10 0.02618352104158106 1102 11 -0.3468206959340421 1102 12 -0.2558284253256886 1102 13 -0.3204171266103586 1102 14 -0.5494213947648987 1102 15 -0.051239932093523066 1102 16 -0.14359430118049232 1102 17 -0.28195402307080286 1102 18 -0.4250217907511953 1102 19 -0.0895114407294173 1102 20 -0.3323817119538077 1102 21 -0.2573970432354429 1102 22 -0.5577211137468439 1102 23 -0.6584089461276861 1102 24 -3.8358364791751 1102 25 -1.844037449267212 1103 1 -0.703960496986884 1103 2 -0.855136916620225 1103 3 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2215 3 0.5531209521912857 2215 4 0.7789261338110727 2215 5 0.7620229674425758 2215 6 1.3385746910347862 2215 7 0.5084825861820228 2215 8 -0.40642170260449717 2215 9 0.13891639235451692 2215 10 -0.10647638519434821 2215 11 -0.2550017887769158 2215 12 -0.2742131345692371 2215 13 0.13764503272876993 2215 14 0.08848167583096256 2215 15 0.09960381376062344 2215 16 0.2712668745514719 2215 17 -0.14917826644630594 2215 18 -0.5700758200090235 2215 19 0.3829180353465418 2215 20 -2.9471744985854222 2215 21 -1.6544705129358945 2215 22 -0.6936229623079697 2215 23 -0.6559994449009849 2215 24 -0.4395440653201419 2215 25 -0.5406860096392805 2216 1 0.12384533626056538 2216 2 -0.8378112326965805 2216 3 -0.22850782558968064 2216 4 -0.4505127716423871 2216 5 -0.273445622992444 2216 6 -1.6482967382983027 2216 7 -9.527339943726478 2216 8 -11.0788472338213 2216 9 -6.176764807840705 2216 10 -3.5217421268882525 2216 11 -2.4545256315014683 2216 12 -2.214895257549877 2216 13 -1.9740210255830668 2216 14 -2.036333049363538 2216 15 -2.8309149556947695 2216 16 -2.0629561606333127 2216 17 -2.2181009042181685 2216 18 -2.1089045797444115 2216 19 -1.4614880394523944 2216 20 -2.3012378303739425 2216 21 -4.689076196473813 2216 22 -3.321672357986049 2216 23 -3.355095935445508 2216 24 -3.490865288974618 2216 25 -2.7744037685421077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63860.0 3 7.0 4 527.0 5 2919.0 6 3453.0 7 2820.0 8 2210.0 9 2140.0 10 1987.0 11 1857.0 12 1915.0 13 1828.0 14 1846.0 15 1884.0 16 1987.0 17 2235.0 18 2511.0 19 3086.0 20 4039.0 21 5192.0 22 6436.0 23 7574.0 24 8986.0 25 10144.0 26 11406.0 27 12613.0 28 14054.0 29 16355.0 30 19364.0 31 23709.0 32 30637.0 33 39661.0 34 53448.0 35 73358.0 36 106547.0 37 169233.0 38 212920.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.015254558755785 18.581624082152437 12.566179857479613 25.83694150161216 2 13.78839509140541 19.954023884624423 42.87895374560244 23.378627278367727 3 18.979254603771377 24.15250107744385 29.786611975111587 27.081632343673185 4 11.313145085715647 16.65378930031533 39.18257941222939 32.850486201739635 5 12.702636053318104 38.08703345576942 36.678910275546095 12.53142021536638 6 31.72784892273081 37.72194341091979 18.52189149433244 12.02831617201696 7 27.48773270973704 31.227575046314172 23.009575211179612 18.275117032769174 8 23.878745153330982 38.5524253680757 20.91223123017272 16.656598248420597 9 25.79446024724473 15.580527585199928 20.386766026022347 38.238246141533004 10 13.560264416394164 29.996830551321814 35.75397722540189 20.688927806882138 11 34.2850406491755 22.66998623216698 24.886574902743725 18.1583982159138 12 22.78899939866148 24.963551963867776 32.77954670323728 19.467901934233467 13 28.838472927768088 22.542906862177826 25.88190378956089 22.736716420493195 14 19.53205862407974 22.991056702173797 28.23406370085323 29.24282097289323 15 22.382984614253175 32.542055023566384 23.093004492646724 21.981955869533714 16 20.767867166637643 28.893873697120988 29.38674240498731 20.951516731254063 17 20.6628660672665 29.59059735602012 28.69668858987379 21.049847986839588 18 20.388287563377112 28.541223243177765 30.860977002543738 20.209512190901382 19 23.314278915574686 27.370328107339287 27.522619234326072 21.79277374275995 20 22.579263577419546 28.56646892456779 28.701986253589375 20.152281244423296 21 22.040706408355422 27.69448238791894 27.47374329590178 22.79106790782385 22 21.78870282705118 29.401375509272626 27.95989197525857 20.850029688417624 23 21.234564963697856 28.860124529391594 28.3099412611494 21.595369245761145 24 22.80770441254882 27.566884739885765 29.00153902227202 20.623871825293392 25 21.793686277945234 28.197931909398555 28.725715725686523 21.282666086969687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 259.0 1 259.0 2 355.0 3 451.0 4 451.0 5 451.0 6 649.5 7 848.0 8 848.0 9 848.0 10 1297.5 11 1747.0 12 1747.0 13 1747.0 14 3159.5 15 4572.0 16 4572.0 17 4572.0 18 8021.0 19 11470.0 20 11470.0 21 11470.0 22 18132.5 23 24795.0 24 24795.0 25 24795.0 26 35827.5 27 46860.0 28 46860.0 29 46860.0 30 61947.5 31 77035.0 32 77035.0 33 77035.0 34 87849.0 35 98663.0 36 98663.0 37 98663.0 38 105459.5 39 112256.0 40 112256.0 41 112256.0 42 116671.5 43 121087.0 44 121087.0 45 121087.0 46 121940.5 47 122794.0 48 122794.0 49 122794.0 50 115539.0 51 108284.0 52 108284.0 53 108284.0 54 94105.0 55 79926.0 56 79926.0 57 79926.0 58 66844.0 59 53762.0 60 53762.0 61 53762.0 62 42665.0 63 31568.0 64 31568.0 65 31568.0 66 24044.5 67 16521.0 68 16521.0 69 16521.0 70 11930.0 71 7339.0 72 7339.0 73 7339.0 74 5109.0 75 2879.0 76 2879.0 77 2879.0 78 1978.0 79 1077.0 80 1077.0 81 1077.0 82 726.5 83 376.0 84 376.0 85 376.0 86 247.0 87 118.0 88 118.0 89 118.0 90 80.0 91 42.0 92 42.0 93 42.0 94 27.0 95 12.0 96 12.0 97 12.0 98 9.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2241691790628366 2 0.13246852115387112 3 0.13690216145371498 4 0.06985686911461285 5 0.019897312565152887 6 0.02032986283830838 7 0.3010549901162262 8 0.2944585984506049 9 0.01708573578964215 10 0.03211685778179569 11 0.013949746309264795 12 0.015571809833597908 13 0.013949746309264795 14 0.015679947401886784 15 0.01362533360439817 16 0.017410148494508774 17 0.01697759822135328 18 0.013409058467820423 19 0.0219519263626415 20 0.015463672265309035 21 0.013733471172687044 22 0.015679947401886784 23 0.015896222538464535 24 0.01459857171899804 25 0.013733471172687044 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 924748.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.88157661310115 #Duplication Level Percentage of deduplicated Percentage of total 1 94.07974212750895 80.79716581264461 2 4.5189386028531 7.761871436616577 3 0.6917955408517491 1.7823747522678373 4 0.2345518730340846 0.8057473861489245 5 0.11653513881337466 0.5004110726059606 6 0.07335341762280917 0.3779824293243654 7 0.05355202831443259 0.32193928357310336 8 0.033588312713159194 0.23076938012639844 9 0.027124221348736828 0.20965238044490303 >10 0.14227428491936925 2.3249650553378514 >50 0.013836171748592106 0.8002801100449005 >100 0.012571180388499614 2.243476547698368 >500 0.0012571175783158846 0.6567403865708175 >1k 8.799823048211193E-4 1.186623966595406 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2386 0.25801623793725426 No Hit CTGTAGGACGTGGAATATGGCAAGA 1820 0.19681037428575135 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1814 0.1961615488760181 No Hit GTCCTACAGTGTGCATTTCTCATTT 1550 0.16761323084775528 No Hit CTTTAGGACGTGAAATATGGCGAGG 1309 0.14155207689013655 No Hit CTGTAGGACCTGGAATATGGCGAGA 1097 0.11862691241289519 No Hit GTATCAACGCAGAGTACTTTTTTTT 1015 0.10975963181320748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0813756828887437E-4 0.0 5 0.0 0.0 0.0 1.0813756828887437E-4 0.0 6 0.0 0.0 0.0 1.0813756828887437E-4 0.0 7 0.0 0.0 0.0 1.0813756828887437E-4 0.0 8 0.0 0.0 0.0 1.0813756828887437E-4 0.0 9 0.0 0.0 0.0 1.0813756828887437E-4 0.0 10 0.0 0.0 0.0 1.0813756828887437E-4 0.0 11 0.0 0.0 0.0 1.0813756828887437E-4 0.0 12 0.0 0.0 0.0 1.0813756828887437E-4 0.0 13 0.0 0.0 0.0 1.0813756828887437E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCACGC 40 0.0052344548 14.267376 8 TAGGACC 390 0.0 13.677697 4 TCCAACG 105 1.4097168E-9 13.543884 18 TAAGGCC 75 1.4368188E-5 12.700719 4 TGGGCCG 60 4.0196825E-4 12.692444 5 ACTATGC 85 3.885103E-6 12.309109 8 CCAACGA 85 4.028898E-6 12.269166 19 GGACCTA 70 1.0694805E-4 12.239141 6 ACTGTTC 315 0.0 12.078202 8 GCCGGTT 55 0.0031138433 12.066371 11 CCGGTTT 55 0.0031138433 12.066371 12 CGTTTCT 55 0.0031138433 12.066371 14 AGGACCT 720 0.0 11.63474 5 GTTCTAT 140 6.2755134E-10 11.588766 1 GTATTAT 100 1.823093E-6 11.452429 1 GTCCTAC 1180 0.0 11.403901 1 TGTAGAA 420 0.0 11.350413 2 GCCTTAT 85 5.0743147E-5 11.227871 1 GTCTAGG 85 5.0743147E-5 11.227871 1 CCGTTTC 110 5.0911876E-7 11.204487 13 >>END_MODULE