FastQCFastQC Report
Thu 26 May 2016
SRR1926803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924748
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT24280.26255801580538696No Hit
CTGTAGGACGTGGAATATGGCAAGA22070.23865961321354576No Hit
GTCCTAAAGTGTGTATTTCTCATTT17790.19237673398590752No Hit
CTTTAGGACGTGAAATATGGCGAGG17390.18805123125435252No Hit
GTATCAACGCAGAGTACTTTTTTTT16370.17702119928888735No Hit
GTCCTACAGTGTGCATTTCTCATTT15200.16436910379908903No Hit
CTGTAGGACCTGGAATATGGCGAGA13620.1472833680094469No Hit
CTGAAGGACCTGGAATATGGCGAGA10700.11570719806909557No Hit
GGTATCAACGCAGAGTACTTTTTTT10380.1122467958838516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT307.607591E-419.0412886
AGGACGG400.00520149414.2809675
ACTAATC400.00520149414.2809673
TAGGACC4900.014.1838174
AGGCGTG905.464535E-712.65368757
GCCGGTT604.1285224E-412.65231811
TGTAGGA11500.012.5947422
ATAACAC1001.3998033E-712.3768393
CTAGAAC1405.0931703E-1112.24082853
GTAGGAC11450.012.1398613
TAGGACG10150.012.00633054
AGGACGT9700.011.9744195
CTGTAGG11950.011.8838251
GGACGTG9550.011.86342056
AAGGCGT657.8732957E-411.7177166
TTAGGAC6200.011.6704673
GGACCTG8250.011.6555786
GACGTGA4900.011.6207327
AGGACCT8850.011.5108365
GTATTAG752.0103295E-411.4371831
Produced by FastQC (version 0.11.5)