##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926803_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 924748 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.780740266537478 31.0 30.0 33.0 25.0 34.0 2 30.070326186160987 31.0 30.0 34.0 26.0 34.0 3 30.14778620770199 31.0 30.0 34.0 26.0 34.0 4 33.746213022358525 35.0 35.0 37.0 28.0 37.0 5 33.69136024084399 37.0 35.0 37.0 28.0 37.0 6 33.60558768442862 37.0 35.0 37.0 28.0 37.0 7 33.583365414145256 37.0 35.0 37.0 28.0 37.0 8 33.60545251246826 37.0 35.0 37.0 28.0 37.0 9 35.14345962359475 39.0 35.0 39.0 29.0 39.0 10 35.036151470454655 39.0 35.0 39.0 28.0 39.0 11 35.06197904726477 39.0 35.0 39.0 29.0 39.0 12 34.96008750492026 39.0 35.0 39.0 28.0 39.0 13 34.97068174248552 39.0 35.0 39.0 28.0 39.0 14 36.052460778503985 40.0 36.0 41.0 27.0 41.0 15 36.03954915285029 40.0 36.0 41.0 27.0 41.0 16 36.02516577489219 40.0 36.0 41.0 27.0 41.0 17 35.99705433263981 40.0 36.0 41.0 27.0 41.0 18 35.968373005402555 40.0 36.0 41.0 27.0 41.0 19 36.03666944940676 40.0 36.0 41.0 27.0 41.0 20 35.989815603818556 40.0 36.0 41.0 27.0 41.0 21 35.94134510158443 40.0 36.0 41.0 27.0 41.0 22 35.8481824237522 40.0 36.0 41.0 27.0 41.0 23 35.77353830448944 40.0 36.0 41.0 27.0 41.0 24 35.71364414954128 40.0 36.0 41.0 27.0 41.0 25 35.58726377348207 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10163154106908934 1101 2 -0.24220151316352556 1101 3 0.0057272127620038304 1101 4 0.08783815077143231 1101 5 0.20263836803585633 1101 6 0.27463518305036416 1101 7 0.1738450929283175 1101 8 0.1767674392455305 1101 9 0.20855863111999184 1101 10 0.2033090683803067 1101 11 0.027429072793673015 1101 12 0.2270046692154466 1101 13 0.25697753390464584 1101 14 0.11388567281100848 1101 15 0.08586070672364343 1101 16 0.1373178245657556 1101 17 0.2274531821093433 1101 18 0.08952908662152481 1101 19 0.1437236067457448 1101 20 0.12782911338221936 1101 21 -0.020924017432854214 1101 22 0.006616256412740995 1101 23 0.12701941604370148 1101 24 0.06702520517132626 1101 25 0.03606223128820574 1102 1 -0.4817115033896151 1102 2 -0.4025374742564871 1102 3 -0.32911820123268143 1102 4 -0.3702754033960005 1102 5 -0.34179911451501255 1102 6 -0.5818546159995535 1102 7 -0.4148636392043059 1102 8 -0.25056104775739385 1102 9 -0.2497467303195009 1102 10 -0.39407361483293357 1102 11 -0.24295204825590133 1102 12 -0.2112099460724437 1102 13 -0.18663858728345417 1102 14 -0.2334431978064586 1102 15 -0.2582244898042205 1102 16 -0.1837249250128039 1102 17 -0.21171514155903282 1102 18 -0.21994022528053847 1102 19 -0.1817413660495859 1102 20 -0.15581015255003905 1102 21 -0.352102715743456 1102 22 -0.1867350641803327 1102 23 -0.23098566045543123 1102 24 0.026313046082108826 1102 25 -0.1550609281611841 1103 1 -0.9030353205156558 1103 2 -0.7682540853597715 1103 3 -0.5440264042659706 1103 4 -0.5231826986332067 1103 5 -0.5633702278173516 1103 6 -0.667093538539504 1103 7 -0.6306968551502123 1103 8 -0.4671690207113315 1103 9 -0.46228683768986656 1103 10 -0.36696964087729356 1103 11 -0.5409048115558974 1103 12 -0.6152981588097646 1103 13 -0.6731593805094178 1103 14 -0.6154750958828714 1103 15 -0.5899832664707318 1103 16 -0.4885127668058544 1103 17 -0.43286052979837564 1103 18 -0.4880797128452983 1103 19 -0.3941327826810408 1103 20 -0.46615746281283066 1103 21 -0.5761342694834184 1103 22 -0.49959133501659636 1103 23 -0.46151734723447646 1103 24 -0.5018114158921847 1103 25 -0.7092328772619965 1104 1 -0.33566485548683644 1104 2 -0.18978862673958474 1104 3 0.0780223627437664 1104 4 -0.025088403467691478 1104 5 -0.0813550773487961 1104 6 -0.24986467743414664 1104 7 -0.22283611052080232 1104 8 -0.26427707716869975 1104 9 -0.32167913044542473 1104 10 -0.30981845086708404 1104 11 -0.3196112654713019 1104 12 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0.06593951774732432 1107 9 0.058462978103221985 1107 10 0.26842556100099557 1107 11 0.1282345987322202 1107 12 -0.0061290256437800394 1107 13 0.08890523552845053 1107 14 0.1260058027970814 1107 15 0.144628899927433 1107 16 0.08324300605141133 1107 17 0.13670474189120796 1107 18 0.1203176245842954 1107 19 -0.12265550569628658 1107 20 0.20433571216499757 1107 21 -0.045116323359259525 1107 22 0.0662098713825614 1107 23 0.007504670174633077 1107 24 0.12749933804484215 1107 25 0.05508468296429925 1108 1 0.6377673760706593 1108 2 0.4732506379362107 1108 3 0.5956244159373263 1108 4 0.6294175630883814 1108 5 0.6596738303240244 1108 6 0.6579712011329164 1108 7 0.508898204792068 1108 8 0.5959063837389422 1108 9 0.37697089779048554 1108 10 0.47608129938638655 1108 11 0.4367910840367841 1108 12 0.4313214381590882 1108 13 0.19975595180415695 1108 14 0.33366300620863854 1108 15 0.4461307959154581 1108 16 0.32478479958527373 1108 17 0.358632765155825 1108 18 0.4773303385696792 1108 19 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0.646548773627913 2214 7 0.4740445816786263 2214 8 0.4276948324172025 2214 9 0.38300274510208254 2214 10 0.24236621148335757 2214 11 0.3824989655804174 2214 12 0.43939563656374503 2214 13 0.37093811240288943 2214 14 0.27432588691235793 2214 15 0.29295125988892323 2214 16 0.39273897438984307 2214 17 0.4487259891914803 2214 18 0.3257482488261516 2214 19 0.41967364083862435 2214 20 0.3036245102223418 2214 21 0.5191214843635947 2214 22 0.6222532672182197 2214 23 0.553347723267855 2214 24 0.2509039129509105 2214 25 0.4671912744976936 2215 1 0.5212716441159593 2215 2 0.48806892778546995 2215 3 0.47693236559337393 2215 4 0.487226750113237 2215 5 0.4918742957654061 2215 6 0.5630770229284181 2215 7 0.29562997687872183 2215 8 0.35418452049810156 2215 9 0.2919491325136363 2215 10 0.23906557567181608 2215 11 0.03635856718995001 2215 12 0.03665476309636517 2215 13 0.14806533088879803 2215 14 0.19571055247615732 2215 15 0.18370830575908315 2215 16 0.2196633251418234 2215 17 0.2537595740936638 2215 18 0.11002970967506798 2215 19 -0.06842847470417723 2215 20 0.17321522802782852 2215 21 0.27307549765669137 2215 22 0.2242329779294181 2215 23 0.246033920128788 2215 24 0.1841470548979558 2215 25 0.0903129976534629 2216 1 0.040801064565702205 2216 2 0.13623135442403012 2216 3 -1.9110812670231958 2216 4 -1.50091238132012 2216 5 -0.45969095336614885 2216 6 -0.04330250025810045 2216 7 -0.20533013008235912 2216 8 0.04516049936837163 2216 9 -0.20898144531408747 2216 10 -0.15060127828611058 2216 11 -0.197828786958695 2216 12 -0.10624368539374984 2216 13 0.021819262979995813 2216 14 -0.2532663860909423 2216 15 -0.19160246786319846 2216 16 -0.20433032609450663 2216 17 -0.20913132806237655 2216 18 -0.17324695779817745 2216 19 -0.1544062376262474 2216 20 -0.20183142390161635 2216 21 -0.07147248103311199 2216 22 -0.08398104266715478 2216 23 -0.18199784009464537 2216 24 -0.2708152749506709 2216 25 -0.07991548401592041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24485.0 3 21.0 4 404.0 5 2818.0 6 3766.0 7 2623.0 8 2077.0 9 1750.0 10 1522.0 11 1443.0 12 1299.0 13 1214.0 14 1222.0 15 1276.0 16 1292.0 17 1414.0 18 1446.0 19 1765.0 20 2195.0 21 2975.0 22 3677.0 23 4570.0 24 5470.0 25 6263.0 26 7445.0 27 8279.0 28 9353.0 29 11661.0 30 14781.0 31 19193.0 32 24585.0 33 32333.0 34 43793.0 35 60877.0 36 89596.0 37 155225.0 38 370640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.56460795489606 19.475432836111732 10.159753954876571 28.800205254115635 2 13.801226313783904 20.873640315931688 40.91696400734403 24.40816936294038 3 18.5713018762627 24.46788030335149 29.209045169607744 27.75177265077806 4 11.579370812372668 17.476328686301564 38.55720693637618 32.38709356494959 5 12.39158739840036 38.97468173737006 35.889599743919156 12.744131120310412 6 31.455049645882514 38.52134252187196 17.826951117900293 12.196656714345231 7 28.10304322995985 31.616342759241313 21.767920185518484 18.512693825280355 8 24.3037966469765 39.506066026574146 19.71723599428113 16.47290133216822 9 25.79456920008825 15.851368947452663 19.886076058884854 38.467985793574236 10 13.898504505732062 30.413826590145458 34.832504425707064 20.855164478415418 11 34.268340292354594 22.792281596497887 24.697333943261537 18.242044167885982 12 23.04029759555337 25.26685734986402 32.26046099194913 19.43238406263348 13 29.05902403048634 22.555155924571395 25.63277108642194 22.75304895852032 14 19.752948911487238 23.298887913247718 27.809738436849823 29.138424738415225 15 21.99420149428855 32.69472630123032 23.17596334461962 22.135108859861514 16 21.007976261538328 29.235118043480142 28.873937003105727 20.882968691875806 17 20.64735473880452 29.80357892096009 28.337449770099532 21.211616570135863 18 20.36799333870418 28.803183580338366 30.597624209917328 20.231198871040124 19 22.876635447316943 27.527204738160815 27.632206430515588 21.96395338400665 20 22.692358849972372 28.537658571084073 28.577453411380244 20.19252916756331 21 21.93248564565617 27.527354096165723 27.456623330553853 23.083536927624255 22 22.068390523688617 29.4506178980652 27.562535955741456 20.918455622504727 23 21.113017938960603 29.04599852716473 28.130180470982875 21.710803062891795 24 22.31689065561645 27.601789893030315 29.163837066963104 20.917482384390127 25 21.671075313237402 28.458804928744303 28.53710076154593 21.33301899647236 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 281.0 1 281.0 2 285.5 3 290.0 4 290.0 5 290.0 6 494.5 7 699.0 8 699.0 9 699.0 10 1197.5 11 1696.0 12 1696.0 13 1696.0 14 2897.5 15 4099.0 16 4099.0 17 4099.0 18 7388.5 19 10678.0 20 10678.0 21 10678.0 22 17228.5 23 23779.0 24 23779.0 25 23779.0 26 34705.0 27 45631.0 28 45631.0 29 45631.0 30 60707.0 31 75783.0 32 75783.0 33 75783.0 34 86332.0 35 96881.0 36 96881.0 37 96881.0 38 103717.5 39 110554.0 40 110554.0 41 110554.0 42 115541.0 43 120528.0 44 120528.0 45 120528.0 46 122839.0 47 125150.0 48 125150.0 49 125150.0 50 118505.0 51 111860.0 52 111860.0 53 111860.0 54 96529.5 55 81199.0 56 81199.0 57 81199.0 58 67906.0 59 54613.0 60 54613.0 61 54613.0 62 43683.0 63 32753.0 64 32753.0 65 32753.0 66 24857.0 67 16961.0 68 16961.0 69 16961.0 70 12050.0 71 7139.0 72 7139.0 73 7139.0 74 4967.0 75 2795.0 76 2795.0 77 2795.0 78 1894.0 79 993.0 80 993.0 81 993.0 82 638.5 83 284.0 84 284.0 85 284.0 86 181.0 87 78.0 88 78.0 89 78.0 90 49.5 91 21.0 92 21.0 93 21.0 94 12.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10975963181320748 2 0.10857011856202987 3 0.015571809833597908 4 0.0 5 0.004325502731554975 6 0.3265754562324006 7 0.022060063930930372 8 0.009732381145998694 9 0.01038120655573194 10 0.004001090026688352 11 0.005298740846154845 12 3.2441270486662313E-4 13 6.488254097332463E-4 14 0.0 15 0.002919714343799608 16 4.3255027315549754E-4 17 0.0 18 3.2441270486662313E-4 19 1.0813756828887439E-4 20 5.406878414443718E-4 21 0.012435820353220552 22 0.0 23 1.0813756828887439E-4 24 0.0 25 0.0054068784144437184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 924748.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.71363925517954 #Duplication Level Percentage of deduplicated Percentage of total 1 91.9929196529275 76.09069170202962 2 6.171173666733399 10.208804649025 3 0.9776999372799966 2.426073597359679 4 0.29863682458436985 0.988053543079356 5 0.14143893647725544 0.5849464584207981 6 0.08399623269113406 0.41685804537651483 7 0.0637723258373408 0.36923888076415007 8 0.04259878788324941 0.28188006189464043 9 0.03198795960983584 0.23812564965094937 >10 0.16161848405404336 2.523736676339765 >50 0.016886142331383365 0.9586736195238562 >100 0.01426170084097671 2.3567848645346774 >500 0.0018317774997503378 0.9565880424842935 >1k 0.001177571249839503 1.5995442095166714 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2428 0.26255801580538696 No Hit CTGTAGGACGTGGAATATGGCAAGA 2207 0.23865961321354576 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1779 0.19237673398590752 No Hit CTTTAGGACGTGAAATATGGCGAGG 1739 0.18805123125435252 No Hit GTATCAACGCAGAGTACTTTTTTTT 1637 0.17702119928888735 No Hit GTCCTACAGTGTGCATTTCTCATTT 1520 0.16436910379908903 No Hit CTGTAGGACCTGGAATATGGCGAGA 1362 0.1472833680094469 No Hit CTGAAGGACCTGGAATATGGCGAGA 1070 0.11570719806909557 No Hit GGTATCAACGCAGAGTACTTTTTTT 1038 0.1122467958838516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 1.0813756828887437E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACCGT 30 7.607591E-4 19.041288 6 AGGACGG 40 0.005201494 14.280967 5 ACTAATC 40 0.005201494 14.280967 3 TAGGACC 490 0.0 14.183817 4 AGGCGTG 90 5.464535E-7 12.6536875 7 GCCGGTT 60 4.1285224E-4 12.652318 11 TGTAGGA 1150 0.0 12.594742 2 ATAACAC 100 1.3998033E-7 12.376839 3 CTAGAAC 140 5.0931703E-11 12.2408285 3 GTAGGAC 1145 0.0 12.139861 3 TAGGACG 1015 0.0 12.0063305 4 AGGACGT 970 0.0 11.974419 5 CTGTAGG 1195 0.0 11.883825 1 GGACGTG 955 0.0 11.8634205 6 AAGGCGT 65 7.8732957E-4 11.717716 6 TTAGGAC 620 0.0 11.670467 3 GGACCTG 825 0.0 11.655578 6 GACGTGA 490 0.0 11.620732 7 AGGACCT 885 0.0 11.510836 5 GTATTAG 75 2.0103295E-4 11.437183 1 >>END_MODULE