FastQCFastQC Report
Thu 26 May 2016
SRR1926802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918202
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT25950.28261755038651626No Hit
GTCCTAAAGTGTGTATTTCTCATTT19890.21661900104769974No Hit
CTGTAGGACGTGGAATATGGCAAGA19640.2138962886162304No Hit
GTCCTACAGTGTGCATTTCTCATTT18710.2037677983711645No Hit
CTTTAGGACGTGAAATATGGCGAGG15030.163689471379936No Hit
CTGTAGGACCTGGAATATGGCGAGA12640.13766034053508924No Hit
GTATCAACGCAGAGTACTTTTTTTT9720.10585905933552747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA556.020109E-717.24039519
TCCAACG855.820766E-1116.73332418
TAGGACC4650.015.5622364
CTCGGTC456.588107E-414.8276452
CACGACT400.00519787814.2824464
GATTAAG500.001453330213.3543851
CCTACAC1800.012.7087143
TGTCGAG751.5057472E-512.64295718
CTACACT2150.012.4002654
GGACCTG7000.012.1027646
AGGACCT7700.011.9915045
TATGTCG951.0619806E-611.97753716
GTCTAGG802.746199E-511.9235591
GTATTAA1209.402356E-911.9235581
GGCGAGG4550.011.8788219
TGACGAA802.918792E-511.85277118
TGTAGGA12500.011.8197512
AATCCCG1800.011.58937619
GACGAAA907.608167E-611.58937619
GTCCTAA6100.011.5717161
Produced by FastQC (version 0.11.5)