##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926802_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 918202 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.168520652318335 31.0 27.0 31.0 16.0 33.0 2 27.835371737373695 31.0 28.0 31.0 16.0 34.0 3 27.658855023186618 31.0 28.0 31.0 12.0 34.0 4 31.298709869941472 35.0 32.0 37.0 17.0 37.0 5 31.116623575204585 35.0 32.0 37.0 16.0 37.0 6 30.78670161903372 35.0 31.0 37.0 11.0 37.0 7 30.854291321517486 35.0 32.0 37.0 11.0 37.0 8 30.503506853611732 35.0 31.0 37.0 10.0 37.0 9 32.62600168590354 37.0 32.0 39.0 15.0 39.0 10 32.74725169407167 37.0 33.0 39.0 13.0 39.0 11 32.93147586260975 37.0 33.0 39.0 15.0 39.0 12 32.76318936356052 37.0 33.0 39.0 11.0 39.0 13 32.9013190997188 37.0 33.0 39.0 11.0 39.0 14 33.87291794180366 39.0 33.0 40.0 11.0 41.0 15 33.43960043650526 39.0 33.0 40.0 10.0 41.0 16 33.52373442880761 38.0 33.0 40.0 10.0 41.0 17 33.7551410256131 39.0 33.0 40.0 10.0 41.0 18 33.81349855478424 39.0 33.0 41.0 10.0 41.0 19 33.16538953302214 39.0 32.0 40.0 7.0 41.0 20 33.42727635095545 39.0 33.0 40.0 2.0 41.0 21 33.4266904232402 39.0 33.0 40.0 2.0 41.0 22 33.453855469711456 39.0 33.0 40.0 2.0 41.0 23 33.44748432262182 39.0 34.0 41.0 2.0 41.0 24 32.125882975641524 38.0 31.0 40.0 2.0 41.0 25 32.70148616535359 39.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2981745360333825 1101 2 -0.27957902513403 1101 3 -0.9855815819128999 1101 4 -0.3923335295727739 1101 5 -0.2582517042722152 1101 6 -2.2341613557406994 1101 7 -0.3687754088488546 1101 8 0.2870179688136467 1101 9 0.22717126036023672 1101 10 -8.793283038623745 1101 11 -4.03146525208701 1101 12 -1.5575561718465067 1101 13 -0.7234671122047445 1101 14 -0.46600069108524167 1101 15 -1.7068727086184978 1101 16 -0.28830776118876855 1101 17 -0.10535178143510393 1101 18 0.10698302666218495 1101 19 -1.027506263655546 1101 20 -0.05437749294932814 1101 21 -0.09561608188916892 1101 22 0.1579845857147646 1101 23 0.10420917989080891 1101 24 -0.1782854265354743 1101 25 0.04717824275182636 1102 1 -0.4041652255258086 1102 2 -0.4131655012999893 1102 3 -0.39014137081752054 1102 4 -0.4561056795706264 1102 5 -0.3518249869769825 1102 6 -1.154424045061507 1102 7 -0.07866943210367339 1102 8 0.2371788646818409 1102 9 0.05582198760956203 1102 10 0.06829288277325674 1102 11 -0.1350081791046449 1102 12 0.1379087099486611 1102 13 -0.07600283108747874 1102 14 -0.3145370750042389 1102 15 0.24459948857548142 1102 16 0.1296525082581752 1102 17 -0.1597522834302154 1102 18 -0.23045797818614489 1102 19 0.2946453431578604 1102 20 -0.08734881057922195 1102 21 -0.23191204447551428 1102 22 -0.37465796802445794 1102 23 -0.5773403545838178 1102 24 -3.7116841908552054 1102 25 -2.3327666235973403 1103 1 -0.7293740664586323 1103 2 -0.8033064689296445 1103 3 -0.3321271000204433 1103 4 -0.48942015920863824 1103 5 -0.6910740297893732 1103 6 -0.12016107952025834 1103 7 -0.21154447561220024 1103 8 0.012553038019813556 1103 9 -0.2112812146180758 1103 10 -0.2869542456639138 1103 11 -0.5731912629890843 1103 12 -0.6855945966994454 1103 13 -0.6721305721247219 1103 14 -0.6563705243603124 1103 15 -0.251584813547737 1103 16 -0.34754032153646364 1103 17 -0.6468868117977706 1103 18 -0.6835949430312596 1103 19 -2.3716834072772244 1103 20 -1.0973427193853524 1103 21 -0.8700168130101531 1103 22 -0.8574459944776436 1103 23 -0.7587709149736526 1103 24 0.5532218307250218 1103 25 -0.06153446209368951 1104 1 -0.46928567168552604 1104 2 -0.1369663410302593 1104 3 -1.4479777930576496 1104 4 -0.3793111802279938 1104 5 -0.4660150501688065 1104 6 -1.6763900953604995 1104 7 -0.3385405038200311 1104 8 0.2751872619845237 1104 9 0.2751204099345088 1104 10 0.08946638461812029 1104 11 0.1258457587213897 1104 12 -3.244636380017596E-4 1104 13 -0.18540738598802875 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0.6152329953904001 2215 4 0.7809759223507662 2215 5 1.1517914401512215 2215 6 1.4763110584419827 2215 7 1.0008357335400326 2215 8 -0.21469868478253318 2215 9 0.256454350081448 2215 10 0.3797659489926204 2215 11 0.033211675266137775 2215 12 -0.0969508074162917 2215 13 0.22216155456308684 2215 14 0.27733466072829316 2215 15 0.41771479108015086 2215 16 0.6607721758270841 2215 17 0.1368190443880195 2215 18 -0.5004405369906166 2215 19 0.5478367007118976 2215 20 -3.0444410868935066 2215 21 -1.4323066157842774 2215 22 -0.617732955634537 2215 23 -0.4122541969840299 2215 24 -0.14784655599569163 2215 25 -0.10319569606884471 2216 1 -0.22363301264065427 2216 2 -1.200902818449073 2216 3 -0.5020928180630868 2216 4 -0.6374665572550349 2216 5 -0.6715145294606444 2216 6 -2.2266112532781257 2216 7 -9.84302517440059 2216 8 -11.10396648234586 2216 9 -6.248181280364292 2216 10 -3.8102863266883027 2216 11 -2.744104039671093 2216 12 -2.3487626252520855 2216 13 -2.1399384053171744 2216 14 -2.1333084724521036 2216 15 -3.2752534526009924 2216 16 -2.3299179866064357 2216 17 -2.705048735344313 2216 18 -2.3745172685689653 2216 19 -1.6308487433656396 2216 20 -2.57364277260848 2216 21 -5.2599310298474435 2216 22 -3.6326878466425683 2216 23 -3.524702632828447 2216 24 -3.4774949596071494 2216 25 -3.159097155810226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61833.0 3 8.0 4 513.0 5 2687.0 6 3403.0 7 2788.0 8 2287.0 9 2001.0 10 1856.0 11 1852.0 12 1823.0 13 1836.0 14 1814.0 15 1952.0 16 2054.0 17 2218.0 18 2552.0 19 3143.0 20 4049.0 21 5173.0 22 6389.0 23 7714.0 24 8931.0 25 10240.0 26 11662.0 27 12596.0 28 13941.0 29 16673.0 30 19937.0 31 23763.0 32 30182.0 33 40100.0 34 53637.0 35 74376.0 36 107040.0 37 169343.0 38 205836.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89604960103045 18.64426760978485 12.421106635666023 26.038576153518683 2 13.691607588600741 19.954264253162734 43.14143730636735 23.212690851869176 3 18.92527645095858 24.289624637396674 29.871098606294577 26.91400030535017 4 11.252185133680047 16.730386109306817 39.39725185382164 32.62017690319149 5 12.528118222351004 38.11692605329635 36.99012744022597 12.36482828412667 6 31.57344337048322 37.91652006061048 18.55820251352679 11.951834055379507 7 27.374885037760045 30.96487453004747 23.344926041869194 18.31531439032329 8 23.695924062938627 38.717852090945335 21.105959071322072 16.480264774793966 9 25.780181978011445 15.554403321049689 20.50362572615283 38.161788974786035 10 13.606476825910791 29.994759532258293 35.95603672467498 20.44272691715594 11 34.144372794213766 22.537471134155375 25.244324866018907 18.073831205611956 12 23.051090056272916 24.85289087672898 32.9686740898406 19.12734497715751 13 29.04622892248704 22.689861008234935 25.77164393708335 22.492266132194676 14 19.349585525527488 23.216779408080345 28.30506628323693 29.128568783155234 15 22.234034789220114 32.93429610595582 23.0451079088888 21.786561195935267 16 20.579338201093012 28.872466947635818 29.888792424508146 20.65940242676302 17 20.509248164528007 29.628766257815737 29.073331735692033 20.788653841964226 18 20.19846305504572 28.461039915909176 31.349320029845707 19.991176999199396 19 23.362952747285068 27.35378478156908 27.51577105794359 21.767491413202265 20 22.71459725366059 28.505634941210563 28.846149656544178 19.933618148584674 21 21.892727985708685 27.735610648773473 27.451745022003397 22.919916343514444 22 21.853241309595393 29.662949988235603 27.89349733776023 20.590311364408773 23 20.8898678030204 29.10424999782141 28.468972488736675 21.536909710421515 24 22.840800534183263 27.48222566192866 29.196644579684694 20.48032922420338 25 21.635199299377696 28.39317633961882 29.030947419559695 20.94067694144379 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 247.0 2 352.0 3 457.0 4 457.0 5 457.0 6 678.5 7 900.0 8 900.0 9 900.0 10 1329.0 11 1758.0 12 1758.0 13 1758.0 14 3102.0 15 4446.0 16 4446.0 17 4446.0 18 7803.0 19 11160.0 20 11160.0 21 11160.0 22 17861.5 23 24563.0 24 24563.0 25 24563.0 26 35912.5 27 47262.0 28 47262.0 29 47262.0 30 63245.5 31 79229.0 32 79229.0 33 79229.0 34 89699.0 35 100169.0 36 100169.0 37 100169.0 38 107000.0 39 113831.0 40 113831.0 41 113831.0 42 117458.0 43 121085.0 44 121085.0 45 121085.0 46 121546.0 47 122007.0 48 122007.0 49 122007.0 50 114186.5 51 106366.0 52 106366.0 53 106366.0 54 91395.0 55 76424.0 56 76424.0 57 76424.0 58 63879.5 59 51335.0 60 51335.0 61 51335.0 62 40828.0 63 30321.0 64 30321.0 65 30321.0 66 22984.5 67 15648.0 68 15648.0 69 15648.0 70 11249.0 71 6850.0 72 6850.0 73 6850.0 74 4774.0 75 2698.0 76 2698.0 77 2698.0 78 1843.0 79 988.0 80 988.0 81 988.0 82 652.0 83 316.0 84 316.0 85 316.0 86 211.0 87 106.0 88 106.0 89 106.0 90 64.5 91 23.0 92 23.0 93 23.0 94 15.5 95 8.0 96 8.0 97 8.0 98 6.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22783657626535336 2 0.13014565422423388 3 0.13308618365022076 4 0.06948362125109726 5 0.022108424943530942 6 0.022761875927083584 7 0.2923104066425471 8 0.29481530207949885 9 0.01949462100932039 10 0.037573431554276726 11 0.017643176555921247 12 0.01916789551754407 13 0.017643176555921247 14 0.018732261528508976 15 0.01731645106414493 16 0.020583705981908118 17 0.01982134650109671 18 0.01600954909703965 19 0.02428659488870641 20 0.019603529506579162 21 0.017643176555921247 22 0.019385712512061615 23 0.019385712512061615 24 0.017969902047697564 25 0.017534268058662473 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 918202.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.17257830814461 #Duplication Level Percentage of deduplicated Percentage of total 1 94.14035021295399 80.18176350468981 2 4.459646828246988 7.596792374110704 3 0.6574908074952508 1.6800056186482342 4 0.24330936253423105 0.8289314293420617 5 0.11807167184767237 0.5028234358209714 6 0.07417420436350136 0.3790564937756784 7 0.05580313431841816 0.3327027779302767 8 0.03701307191903051 0.25219990131588915 9 0.025426286633389493 0.1949060150430927 >10 0.1546021780011109 2.437609109513852 >50 0.018784408568764357 1.101907511757898 >100 0.013028658489252864 2.440652789563721 >500 0.001532783085560434 0.8536953189204728 >1k 7.66391542780217E-4 1.2169537195672753 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2595 0.28261755038651626 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1989 0.21661900104769974 No Hit CTGTAGGACGTGGAATATGGCAAGA 1964 0.2138962886162304 No Hit GTCCTACAGTGTGCATTTCTCATTT 1871 0.2037677983711645 No Hit CTTTAGGACGTGAAATATGGCGAGG 1503 0.163689471379936 No Hit CTGTAGGACCTGGAATATGGCGAGA 1264 0.13766034053508924 No Hit GTATCAACGCAGAGTACTTTTTTTT 972 0.10585905933552747 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.0890849725877312E-4 3 0.0 0.0 0.0 0.0 1.0890849725877312E-4 4 0.0 0.0 0.0 0.0 1.0890849725877312E-4 5 0.0 0.0 0.0 0.0 1.0890849725877312E-4 6 0.0 0.0 0.0 1.0890849725877312E-4 1.0890849725877312E-4 7 0.0 0.0 0.0 1.0890849725877312E-4 1.0890849725877312E-4 8 0.0 0.0 0.0 1.0890849725877312E-4 1.0890849725877312E-4 9 0.0 0.0 0.0 2.1781699451754624E-4 1.0890849725877312E-4 10 0.0 0.0 0.0 2.1781699451754624E-4 1.0890849725877312E-4 11 0.0 0.0 0.0 2.1781699451754624E-4 1.0890849725877312E-4 12 0.0 1.0890849725877312E-4 0.0 2.1781699451754624E-4 1.0890849725877312E-4 13 0.0 1.0890849725877312E-4 0.0 2.1781699451754624E-4 1.0890849725877312E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 55 6.020109E-7 17.240395 19 TCCAACG 85 5.820766E-11 16.733324 18 TAGGACC 465 0.0 15.562236 4 CTCGGTC 45 6.588107E-4 14.827645 2 CACGACT 40 0.005197878 14.282446 4 GATTAAG 50 0.0014533302 13.354385 1 CCTACAC 180 0.0 12.708714 3 TGTCGAG 75 1.5057472E-5 12.642957 18 CTACACT 215 0.0 12.400265 4 GGACCTG 700 0.0 12.102764 6 AGGACCT 770 0.0 11.991504 5 TATGTCG 95 1.0619806E-6 11.977537 16 GTCTAGG 80 2.746199E-5 11.923559 1 GTATTAA 120 9.402356E-9 11.923558 1 GGCGAGG 455 0.0 11.87882 19 TGACGAA 80 2.918792E-5 11.852771 18 TGTAGGA 1250 0.0 11.819751 2 AATCCCG 180 0.0 11.589376 19 GACGAAA 90 7.608167E-6 11.589376 19 GTCCTAA 610 0.0 11.571716 1 >>END_MODULE