FastQCFastQC Report
Thu 26 May 2016
SRR1926802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918202
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT26410.28762734126041983No Hit
CTGTAGGACGTGGAATATGGCAAGA24150.2630140208799371No Hit
GTCCTAAAGTGTGTATTTCTCATTT20180.21977734746820415No Hit
CTTTAGGACGTGAAATATGGCGAGG19510.21248047815186638No Hit
GTCCTACAGTGTGCATTTCTCATTT19460.21193593566557248No Hit
GTATCAACGCAGAGTACTTTTTTTT15960.1738179616250019No Hit
CTGTAGGACCTGGAATATGGCGAGA15250.166085458319629No Hit
CTGAAGGACCTGGAATATGGCGAGA10830.11794790253125129No Hit
GGTATCAACGCAGAGTACTTTTTTT9320.10150271944517657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5500.015.0597724
TCGAACT456.8186765E-414.76053719
CTTAGAC653.2881944E-614.6493933
CTCGAAC500.001511972713.28448418
CGCCACC802.0244388E-613.0465514
TGTAGGA15100.013.00108052
GTAGGAC14800.012.8033733
GCCTCGA604.1296525E-412.6518916
CTGTAGG15600.012.3427231
GCGCCAC853.9925053E-612.27910713
TGGCGAA550.003093728312.07680318
GGACCTG9150.012.0697626
GTATTAG802.766458E-511.9150051
GTCCTAA5450.011.8931421
TCCAACG1201.0162694E-811.86114718
AGGACCT9750.011.8152415
GTATAGC1950.011.7316971
GTCCTAC13000.011.6583741
TTATACT1156.84995E-811.5902294
CAAGACT1251.7696948E-811.4246544
Produced by FastQC (version 0.11.5)