##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926802_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 918202 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.849202027440587 31.0 30.0 33.0 26.0 34.0 2 30.177377091315417 31.0 30.0 34.0 26.0 34.0 3 30.249212047022333 31.0 30.0 34.0 26.0 34.0 4 33.80651207468509 35.0 35.0 37.0 28.0 37.0 5 33.77165155379753 37.0 35.0 37.0 29.0 37.0 6 33.708018497019175 37.0 35.0 37.0 29.0 37.0 7 33.68635659691441 37.0 35.0 37.0 29.0 37.0 8 33.703152465361654 37.0 35.0 37.0 29.0 37.0 9 35.236856378008326 39.0 35.0 39.0 29.0 39.0 10 35.144139307037015 39.0 35.0 39.0 29.0 39.0 11 35.17142197468531 39.0 35.0 39.0 29.0 39.0 12 35.06473629985559 39.0 35.0 39.0 29.0 39.0 13 35.08780638682991 39.0 35.0 39.0 29.0 39.0 14 36.17726491556324 40.0 36.0 41.0 29.0 41.0 15 36.16788898303423 40.0 36.0 41.0 29.0 41.0 16 36.15009769092204 40.0 36.0 41.0 29.0 41.0 17 36.114656687744095 40.0 36.0 41.0 28.0 41.0 18 36.08581227224511 40.0 36.0 41.0 28.0 41.0 19 36.15068688589221 40.0 37.0 41.0 28.0 41.0 20 36.11446827604383 40.0 37.0 41.0 28.0 41.0 21 36.05281844300056 40.0 36.0 41.0 27.0 41.0 22 35.97306910679785 40.0 36.0 41.0 27.0 41.0 23 35.90578108085149 40.0 36.0 41.0 27.0 41.0 24 35.838483253140375 40.0 36.0 41.0 27.0 41.0 25 35.718991028118 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.25826453505630553 1101 2 -0.10292724248162699 1101 3 0.12125471708788638 1101 4 0.007883110691942363 1101 5 0.09256565426647256 1101 6 0.08515149598520111 1101 7 -0.049031386757221185 1101 8 -0.024849349993779413 1101 9 -0.03086582934878379 1101 10 0.03444485791536067 1101 11 0.02866216019149448 1101 12 0.131714364311172 1101 13 0.0814752690870435 1101 14 0.024944754397004942 1101 15 0.017303840893553968 1101 16 -0.10375223726926919 1101 17 0.05401379016706187 1101 18 0.10426939874756158 1101 19 0.07820746725660399 1101 20 0.10953114786783402 1101 21 0.09724127097653223 1101 22 -0.08424974870362689 1101 23 0.02286331918119089 1101 24 0.07263892129646621 1101 25 -0.0682499835590491 1102 1 -0.7063150134533522 1102 2 -0.5992073300089764 1102 3 -0.3298828167002448 1102 4 -0.49199139053086327 1102 5 -0.5567129959244568 1102 6 -0.6346998362325849 1102 7 -0.5437277226219805 1102 8 -0.43324489639614683 1102 9 -0.40556656303394334 1102 10 -0.41830324351339954 1102 11 -0.4525264312188142 1102 12 -0.27595661436475893 1102 13 -0.33817869753242746 1102 14 -0.12160831978671638 1102 15 -0.4270374514220663 1102 16 -0.4266150038200891 1102 17 -0.4442163552828049 1102 18 -0.4848894376535142 1102 19 -0.335762584659129 1102 20 -0.3816093893098369 1102 21 -0.4997896639357293 1102 22 -0.3590041143448488 1102 23 -0.48534880593143725 1102 24 -0.35935629473305397 1102 25 -0.4028447404990203 1103 1 -0.5108616545950149 1103 2 -0.5446215311924085 1103 3 -0.3308965653949869 1103 4 -0.324335630001805 1103 5 -0.698676970863545 1103 6 -0.5549748101274687 1103 7 -0.584195004209171 1103 8 -0.3946006221183893 1103 9 -0.4079682337614088 1103 10 -0.3716882069662333 1103 11 -0.47543367103781264 1103 12 -0.5336445712042988 1103 13 -0.30393748625501615 1103 14 -0.4775385321083334 1103 15 -0.4933052899184105 1103 16 -0.4775939456926963 1103 17 -0.5087395018195267 1103 18 -0.44178118843667136 1103 19 -0.40168670578687227 1103 20 -0.37104116793845066 1103 21 -0.47929714740248386 1103 22 -0.31808869492264336 1103 23 -0.438890400083892 1103 24 -0.4494668063933389 1103 25 -0.5033581411255454 1104 1 -0.022990338954858913 1104 2 -0.014636313426425573 1104 3 0.10577020081534272 1104 4 0.13669409231403762 1104 5 0.13040424823348218 1104 6 0.055523455679704625 1104 7 -0.1373454545020465 1104 8 0.020551914325260157 1104 9 -0.03771593323624245 1104 10 0.05874837021067947 1104 11 -0.04758718443141419 1104 12 0.13695264811456553 1104 13 0.20166077827231277 1104 14 0.0790835034458155 1104 15 0.2632149977912235 1104 16 0.1094281913959918 1104 17 0.15747370790901982 1104 18 0.02857275327909292 1104 19 0.20339514043523366 1104 20 0.13445204247268094 1104 21 0.05617114865174244 1104 22 -0.03885058790182683 1104 23 0.024481975696282632 1104 24 0.06452565530723575 1104 25 -0.15157030137946492 1105 1 -0.1611533010815691 1105 2 -0.08105786381680602 1105 3 0.09295316822753463 1105 4 -0.10054801602803565 1105 5 -0.16996137356732532 1105 6 -0.2264676821609939 1105 7 -0.5268211913261922 1105 8 -0.7134155055650808 1105 9 -0.41836748411412117 1105 10 -0.3007817865320277 1105 11 -0.34783991248320234 1105 12 -0.31592678392724594 1105 13 -0.2053704496781279 1105 14 -0.2492956758679412 1105 15 -0.4911179412692448 1105 16 -0.2858179554633793 1105 17 -0.1790394660217629 1105 18 -0.23486327060306422 1105 19 -0.03586725286093184 1105 20 -0.11425801815639858 1105 21 -0.17699292958362633 1105 22 -0.3646352636017056 1105 23 -0.4258763336685121 1105 24 -0.3945588277035412 1105 25 -0.40246793497254174 1106 1 0.28965782919683747 1106 2 0.19114043436291794 1106 3 0.40487222442195403 1106 4 0.41620069396794435 1106 5 0.3228850711235154 1106 6 0.46620297343634576 1106 7 0.28672618711792097 1106 8 0.34123116088269256 1106 9 0.184666393171959 1106 10 0.16871299662354033 1106 11 0.34312286114540314 1106 12 0.1559376160030439 1106 13 0.014162345343201821 1106 14 -0.044810528278347306 1106 15 0.1412833225937149 1106 16 0.1977864281508559 1106 17 -0.1186725820602419 1106 18 0.06631396066819661 1106 19 -0.0228330977472595 1106 20 0.13754217834348026 1106 21 0.20012266437387893 1106 22 0.28005153630434165 1106 23 0.15812382797663105 1106 24 0.2726203887721397 1106 25 0.24937418823641622 1107 1 0.32684324985196866 1107 2 0.03538650752603445 1107 3 0.040670404588272646 1107 4 0.2070982453234791 1107 5 0.2972435867085963 1107 6 0.2324570265125132 1107 7 0.14748376197185564 1107 8 0.08559263861786093 1107 9 -0.0865189439863272 1107 10 -0.018583355558213555 1107 11 -0.02202923233925702 1107 12 5.48987876136664E-4 1107 13 -0.12004144263046612 1107 14 0.03830064379871345 1107 15 -0.11642820772458151 1107 16 0.042817769289158036 1107 17 -0.01144324603910718 1107 18 -0.1535869166451178 1107 19 -0.05312953143783972 1107 20 0.024237949625735666 1107 21 -0.01169442584048852 1107 22 0.030211571929662284 1107 23 0.020673603032150822 1107 24 -0.05224845159580127 1107 25 -0.14332093097521437 1108 1 0.3186315852978794 1108 2 0.39227311691839617 1108 3 0.2862731149461588 1108 4 0.4493247448618405 1108 5 0.523885919619417 1108 6 0.4776646067513113 1108 7 0.48937694382560437 1108 8 0.37455990150066754 1108 9 0.4214051753820911 1108 10 0.37205166940149326 1108 11 0.37131106467339947 1108 12 0.12596172280250784 1108 13 0.26402307703214234 1108 14 0.22961990893493578 1108 15 0.20099820418096215 1108 16 0.25027868661697994 1108 17 0.40712278372475197 1108 18 0.30034717095640673 1108 19 0.25234126023077863 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2214 8 0.3517410141357331 2214 9 0.3484528491221397 2214 10 0.2066664249927257 2214 11 0.308767158073195 2214 12 0.2921870003665603 2214 13 0.3687359046357841 2214 14 0.1412096716813238 2214 15 0.16411015532522555 2214 16 0.32680022454670876 2214 17 0.360810845218154 2214 18 0.4316627520846552 2214 19 0.25784236230968105 2214 20 0.37061801861940324 2214 21 0.3630728463378432 2214 22 0.4107544078264951 2214 23 0.4027847309164372 2214 24 0.5205177806455978 2214 25 0.5595520345782745 2215 1 0.5220316946401269 2215 2 0.501375949317012 2215 3 0.3036085675397757 2215 4 0.4152738681325516 2215 5 0.4599881401359269 2215 6 0.49619395355811946 2215 7 0.3017237470131846 2215 8 0.5127035996581384 2215 9 0.31220820727599374 2215 10 0.3990731432046104 2215 11 0.09515808328692543 2215 12 0.24438715827066915 2215 13 0.30733138595657294 2215 14 0.36970968181444164 2215 15 0.5447483173248386 2215 16 0.44960063797778815 2215 17 0.42819605751122225 2215 18 0.37847166180895186 2215 19 0.4671118768558742 2215 20 0.3424065650952528 2215 21 0.4758117237353474 2215 22 0.43578449455301893 2215 23 0.40629466934590397 2215 24 0.4913227682077377 2215 25 0.4143639057592239 2216 1 -0.19428186686138105 2216 2 -0.11653537233701883 2216 3 -1.6850143137435047 2216 4 -1.2517408458685537 2216 5 -0.37366366508101123 2216 6 -0.024895914663957797 2216 7 -0.27883120846076537 2216 8 -0.19957698478705765 2216 9 -0.14981648522503832 2216 10 -0.19240844114800382 2216 11 -0.2233986690028118 2216 12 -0.14005169684219965 2216 13 -0.10840901188038998 2216 14 -0.19691566494700652 2216 15 -0.33491560268256393 2216 16 -0.2523113934870622 2216 17 -0.18729136306369298 2216 18 -0.17494546006248157 2216 19 -0.35265489713967924 2216 20 -0.08099858943433702 2216 21 -0.07678157180998824 2216 22 -0.03561475860254859 2216 23 -0.06631674866408588 2216 24 -0.2583253267903203 2216 25 -0.36449273302936547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23405.0 3 21.0 4 381.0 5 2609.0 6 3430.0 7 2440.0 8 1805.0 9 1645.0 10 1525.0 11 1277.0 12 1229.0 13 1162.0 14 1147.0 15 1220.0 16 1220.0 17 1274.0 18 1455.0 19 1665.0 20 2146.0 21 2830.0 22 3604.0 23 4395.0 24 5169.0 25 6025.0 26 7378.0 27 8051.0 28 9387.0 29 11315.0 30 14342.0 31 18871.0 32 24267.0 33 31856.0 34 43493.0 35 60388.0 36 89450.0 37 154531.0 38 371794.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.532920655240055 19.49462380833476 10.061073013927613 28.91138252249757 2 13.566965303013948 21.10178167818581 41.02122945716656 24.310023561633678 3 18.489979892250346 24.740810453415595 29.26461842113863 27.504591233195434 4 11.634912579149251 17.669096778268834 38.67416973607114 32.02182090651077 5 12.330051723743583 39.1007104526835 36.042049911397626 12.527187912175282 6 31.246400379442356 38.838307503494434 17.899034673293794 12.016257443769419 7 27.859998474928922 31.794463991982486 21.917450081155568 18.428087451933028 8 24.161040930092735 39.64733765965002 19.87055904463774 16.321062365619508 9 25.888626753890094 15.763934726206891 19.93111876458171 38.416319755321304 10 13.806623624434744 30.428853978792553 35.017556699863206 20.7469656969095 11 34.154435629675135 22.693638118141145 24.997277151159498 18.154649101024226 12 23.25547456384822 25.156910088313992 32.45030227586334 19.13731307197444 13 29.23088651709867 22.66216510564147 25.636898279241993 22.47005009801786 14 19.640492267479853 23.5815726421259 27.80636027009366 28.97157482030059 15 21.89241231490396 33.21752351411283 23.034573042959273 21.855491128023942 16 20.786039612371187 29.073467814131593 29.41805580060074 20.722436772896476 17 20.41295968965401 30.052243463032603 28.628916762233974 20.905880085079414 18 20.30842953604879 28.588107166194725 31.049989109126553 20.053474188629927 19 22.748747549553475 27.659986930951863 27.627096493138747 21.964169026355915 20 22.784083607239396 28.614198680458898 28.689672597849263 19.91204511445244 21 21.73009935888359 27.655579106968194 27.39318538786127 23.22113614628695 22 22.031208818974473 29.83188884363136 27.473148610000848 20.66375372739332 23 20.983617983842333 29.322523801952077 28.221894528654918 21.471963685550673 24 22.44342748109893 27.72516287265765 29.254673808159858 20.57673583808356 25 21.72851365730984 28.69630660685093 28.653394368912373 20.921785366926862 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 312.0 1 312.0 2 300.5 3 289.0 4 289.0 5 289.0 6 514.5 7 740.0 8 740.0 9 740.0 10 1181.5 11 1623.0 12 1623.0 13 1623.0 14 2842.0 15 4061.0 16 4061.0 17 4061.0 18 7138.5 19 10216.0 20 10216.0 21 10216.0 22 16873.5 23 23531.0 24 23531.0 25 23531.0 26 34794.5 27 46058.0 28 46058.0 29 46058.0 30 62177.0 31 78296.0 32 78296.0 33 78296.0 34 88391.5 35 98487.0 36 98487.0 37 98487.0 38 105359.0 39 112231.0 40 112231.0 41 112231.0 42 116798.5 43 121366.0 44 121366.0 45 121366.0 46 122889.5 47 124413.0 48 124413.0 49 124413.0 50 117058.5 51 109704.0 52 109704.0 53 109704.0 54 93561.0 55 77418.0 56 77418.0 57 77418.0 58 64944.0 59 52470.0 60 52470.0 61 52470.0 62 41470.0 63 30470.0 64 30470.0 65 30470.0 66 23213.0 67 15956.0 68 15956.0 69 15956.0 70 11368.5 71 6781.0 72 6781.0 73 6781.0 74 4659.5 75 2538.0 76 2538.0 77 2538.0 78 1709.5 79 881.0 80 881.0 81 881.0 82 572.5 83 264.0 84 264.0 85 264.0 86 172.5 87 81.0 88 81.0 89 81.0 90 45.5 91 10.0 92 10.0 93 10.0 94 7.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12012607247642675 2 0.11250247766831263 3 0.016118457594298422 4 0.0 5 0.005989967349232522 6 0.3457844787966047 7 0.023088601418859902 8 0.010890849725877312 9 0.010890849725877312 10 0.0037028889067982863 11 0.005009790873903564 12 1.0890849725877312E-4 13 2.1781699451754624E-4 14 2.1781699451754624E-4 15 0.003267254917763194 16 4.356339890350925E-4 17 1.0890849725877312E-4 18 2.1781699451754624E-4 19 2.1781699451754624E-4 20 4.356339890350925E-4 21 0.012633385682017682 22 0.0 23 0.0 24 0.0 25 0.0053365163656798826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 918202.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.89510030651151 #Duplication Level Percentage of deduplicated Percentage of total 1 91.94097804018602 75.29515618879807 2 6.241612341581103 10.223149375762892 3 0.9299016026809709 2.2846315508023176 4 0.31095606483638544 1.0186311248277553 5 0.1485259781171805 0.6081774938014615 6 0.08493718734077274 0.4173563686215327 7 0.05496439318308264 0.31509201451105634 8 0.04392824449826486 0.28780063915794907 9 0.03164905221165762 0.2332712074931749 >10 0.17301808584274783 2.609385514933599 >50 0.021165851158922118 1.1966006739331707 >100 0.014637300610512218 2.4204399184621557 >500 0.0026613269659471156 1.4366150443127659 >1k 0.0010645307863788463 1.6536928845821302 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2641 0.28762734126041983 No Hit CTGTAGGACGTGGAATATGGCAAGA 2415 0.2630140208799371 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2018 0.21977734746820415 No Hit CTTTAGGACGTGAAATATGGCGAGG 1951 0.21248047815186638 No Hit GTCCTACAGTGTGCATTTCTCATTT 1946 0.21193593566557248 No Hit GTATCAACGCAGAGTACTTTTTTTT 1596 0.1738179616250019 No Hit CTGTAGGACCTGGAATATGGCGAGA 1525 0.166085458319629 No Hit CTGAAGGACCTGGAATATGGCGAGA 1083 0.11794790253125129 No Hit GGTATCAACGCAGAGTACTTTTTTT 932 0.10150271944517657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 550 0.0 15.059772 4 TCGAACT 45 6.8186765E-4 14.760537 19 CTTAGAC 65 3.2881944E-6 14.649393 3 CTCGAAC 50 0.0015119727 13.284484 18 CGCCACC 80 2.0244388E-6 13.04655 14 TGTAGGA 1510 0.0 13.0010805 2 GTAGGAC 1480 0.0 12.803373 3 GCCTCGA 60 4.1296525E-4 12.65189 16 CTGTAGG 1560 0.0 12.342723 1 GCGCCAC 85 3.9925053E-6 12.279107 13 TGGCGAA 55 0.0030937283 12.076803 18 GGACCTG 915 0.0 12.069762 6 GTATTAG 80 2.766458E-5 11.915005 1 GTCCTAA 545 0.0 11.893142 1 TCCAACG 120 1.0162694E-8 11.861147 18 AGGACCT 975 0.0 11.815241 5 GTATAGC 195 0.0 11.731697 1 GTCCTAC 1300 0.0 11.658374 1 TTATACT 115 6.84995E-8 11.590229 4 CAAGACT 125 1.7696948E-8 11.424654 4 >>END_MODULE