FastQCFastQC Report
Thu 26 May 2016
SRR1926801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945087
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT24820.2626213248092504No Hit
CTGTAGGACGTGGAATATGGCAAGA18370.19437363967550078No Hit
GTCCTAAAGTGTGTATTTCTCATTT17470.18485070686614036No Hit
GTCCTACAGTGTGCATTTCTCATTT16220.17162441129758424No Hit
CTTTAGGACGTGAAATATGGCGAGG13930.14739383781598941No Hit
CTGTAGGACCTGGAATATGGCGAGA10710.11332290043138886No Hit
GTATCAACGCAGAGTACTTTTTTTT9460.10009660486283273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTC350.002123580516.3377762
TCGGTCG350.002123580516.3377763
TAGGACC3900.013.4309784
ATAGGAC1900.012.5399593
GTACTAG550.002983729612.1353691
TGTAGGA11200.011.99805452
TAATACT1552.3646862E-1111.6743164
TAGACTC907.277218E-611.6358525
GTCCTAG1655.456968E-1211.5574941
AGGACCT7550.011.3486335
AGGACGT9900.011.3473615
CTGTAGG11200.011.3227341
GGACGTG9550.011.264796
GGCGAGG4250.011.15583119
GTCCTAC12650.011.1554961
AGGACAG2850.011.0234385
TGTAGAA4150.011.0230782
GTAGGAC11250.011.0128713
GACGTGG4950.010.9627047
TAGGACG10600.010.871494
Produced by FastQC (version 0.11.5)