##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926801_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 945087 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.42660516968279 31.0 28.0 31.0 16.0 33.0 2 28.074467218361907 31.0 28.0 31.0 16.0 34.0 3 27.901914850167234 31.0 28.0 33.0 16.0 34.0 4 31.55483357616812 35.0 32.0 37.0 18.0 37.0 5 31.387054313518227 35.0 32.0 37.0 17.0 37.0 6 31.06499719073482 35.0 32.0 37.0 11.0 37.0 7 31.132264013789207 35.0 32.0 37.0 13.0 37.0 8 30.807612420867073 35.0 32.0 37.0 11.0 37.0 9 32.93984574965056 37.0 33.0 39.0 17.0 39.0 10 33.05228090112339 37.0 33.0 39.0 17.0 39.0 11 33.24701641224564 38.0 33.0 39.0 17.0 39.0 12 33.06943276121669 38.0 33.0 39.0 17.0 39.0 13 33.2111043745179 38.0 33.0 39.0 17.0 39.0 14 34.21067266823054 39.0 34.0 41.0 17.0 41.0 15 33.77240613827087 39.0 33.0 41.0 10.0 41.0 16 33.858580215366416 39.0 33.0 40.0 11.0 41.0 17 34.0991908681423 39.0 34.0 41.0 17.0 41.0 18 34.14248106259001 39.0 34.0 41.0 15.0 41.0 19 33.528815865629305 39.0 33.0 41.0 10.0 41.0 20 33.78704817651708 39.0 33.0 41.0 10.0 41.0 21 33.79022354555718 39.0 34.0 41.0 8.0 41.0 22 33.81884313296025 39.0 34.0 41.0 2.0 41.0 23 33.81398643722747 39.0 34.0 41.0 2.0 41.0 24 32.49334717332901 38.0 32.0 40.0 2.0 41.0 25 33.07508515089087 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.46990525484619994 1101 2 -0.45920586853372924 1101 3 -1.2542588130403232 1101 4 -0.6810787714715651 1101 5 -0.6619359299131666 1101 6 -2.6873670563628096 1101 7 -0.854924027178928 1101 8 -0.2844837679308476 1101 9 -0.6300365342044785 1101 10 -9.385128413965152 1101 11 -4.131382471278645 1101 12 -1.8506994502035852 1101 13 -0.9517154516263204 1101 14 -0.8858423247712537 1101 15 -2.4115346775563857 1101 16 -0.8174592955183968 1101 17 -0.7614749013400512 1101 18 -0.6511598513478063 1101 19 -1.1422712660288425 1101 20 -0.6375334268803243 1101 21 -0.6132633793898918 1101 22 -0.5058219700389088 1101 23 -0.4528513513084178 1101 24 -0.6851621973682533 1101 25 -0.4932928420054026 1102 1 -0.973730605123162 1102 2 -0.7016457512316769 1102 3 -0.9549821756991683 1102 4 -0.9364661940847938 1102 5 -0.8721750957652361 1102 6 -1.2880708686795224 1102 7 -0.5405440988635135 1102 8 -0.21296079627884268 1102 9 -0.3346386847421101 1102 10 -0.1916139141280695 1102 11 -0.3925281213275227 1102 12 -0.564294758121477 1102 13 -0.7319070449792093 1102 14 -0.9478925039827288 1102 15 -0.3211703895146769 1102 16 -0.5114625539087498 1102 17 -0.7210682542237237 1102 18 -0.9161373684543577 1102 19 -0.5848440910532773 1102 20 -0.5169860173006526 1102 21 -0.6393278955189245 1102 22 -0.6207206340988591 1102 23 -1.0389080473006018 1102 24 -4.336120815810741 1102 25 -2.687732724703352 1103 1 -0.6706904380991006 1103 2 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18 0.2796164456536161 2213 19 0.95162497385693 2213 20 -0.6877285720492878 2213 21 0.38496698710225274 2213 22 0.11586008612482601 2213 23 0.19652656397001778 2213 24 -0.1380860431559725 2213 25 0.2632426172996887 2214 1 0.6679451420481755 2214 2 0.28434080276819884 2214 3 0.023409649790664844 2214 4 0.3747052252837513 2214 5 0.449886075996794 2214 6 0.24397543161448 2214 7 0.6767725948733379 2214 8 -2.39358950986259 2214 9 -1.0802749025919844 2214 10 -0.5174155684932202 2214 11 -0.1793699560691877 2214 12 -1.2148262246401131 2214 13 -0.4348655761990088 2214 14 -0.19026042547233857 2214 15 -1.5874656121412372 2214 16 -0.5057990602788109 2214 17 -0.2613713032173308 2214 18 -0.3016017077705442 2214 19 0.6431499055338179 2214 20 -1.394393036359439 2214 21 -0.7296381403841536 2214 22 -0.27698275571594166 2214 23 -0.8354046858005688 2214 24 -2.311068217415766 2214 25 -1.156175743679892 2215 1 1.0767068322536915 2215 2 0.4171664095946461 2215 3 0.7050271042943734 2215 4 1.0503952179536427 2215 5 1.2398922581589247 2215 6 1.5645426599371497 2215 7 0.9418689684433339 2215 8 -0.18537500677330598 2215 9 0.5061085564772014 2215 10 0.37213896905833366 2215 11 0.2571480875933787 2215 12 0.088004057670652 2215 13 0.3427726943740197 2215 14 0.30085436065713367 2215 15 0.28956077983175277 2215 16 0.8493906675266913 2215 17 0.15141656491726962 2215 18 -0.3491015194358198 2215 19 0.7165418558086714 2215 20 -3.254648669361419 2215 21 -1.4817011529983972 2215 22 -0.5442064148623729 2215 23 -0.2568224696209995 2215 24 -0.36694922335040303 2215 25 -0.10800749320785741 2216 1 -0.2478098407304543 2216 2 -1.199629109841343 2216 3 -0.4546815922941043 2216 4 -0.6676043577023592 2216 5 -0.5889512932029888 2216 6 -1.8623791852758274 2216 7 -10.001129872159868 2216 8 -11.349123712484385 2216 9 -6.219556229248951 2216 10 -3.737619115131835 2216 11 -2.713890037410099 2216 12 -2.410058581542387 2216 13 -2.2219850600357027 2216 14 -2.2691155140978125 2216 15 -3.465550618413495 2216 16 -2.4929921590970032 2216 17 -2.507342522917966 2216 18 -2.3277508181954474 2216 19 -1.591141313389361 2216 20 -2.6027315324596216 2216 21 -5.058899974053169 2216 22 -3.6014523269754903 2216 23 -3.817599416458009 2216 24 -4.115319988750947 2216 25 -3.3953730083447837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58649.0 3 4.0 4 506.0 5 2575.0 6 3307.0 7 2640.0 8 2126.0 9 1932.0 10 1907.0 11 1834.0 12 1811.0 13 1779.0 14 1800.0 15 1795.0 16 1969.0 17 2154.0 18 2437.0 19 3070.0 20 3988.0 21 5146.0 22 6274.0 23 7624.0 24 8669.0 25 10321.0 26 11521.0 27 12573.0 28 13898.0 29 16679.0 30 19869.0 31 23920.0 32 31064.0 33 40598.0 34 54667.0 35 75596.0 36 110621.0 37 176492.0 38 223272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68230083615091 18.846909296080568 12.750134691990175 25.72065517577835 2 13.796950657441645 20.04640764560664 42.922198323815174 23.234443373136543 3 19.027335848752728 24.1785417984507 29.757814064521458 27.036308288275112 4 11.313276982420252 16.83994413614855 39.32186197308667 32.52491690834453 5 12.596506044625347 38.03515545212189 36.776065760105155 12.592272743147609 6 31.52377061845305 37.83201715302898 18.578779264815758 12.065432963702209 7 27.256249880616085 31.05509912579138 23.289308082662487 18.39934291093005 8 23.749720120250135 38.52949322399187 21.162941480460415 16.557845175297576 9 25.62034023864328 15.673201576844722 20.62586977802471 38.08058840648729 10 13.659115903387207 30.101475399598414 35.76428324200512 20.47512545500926 11 34.06508048548446 22.723669854479535 25.062198860684077 18.149050799351926 12 22.731693378979728 25.01595330945187 32.89059151590833 19.361761795660065 13 28.87222739586861 22.68847358618354 25.916335082966473 22.522963934981373 14 19.475439814422867 23.209841006451175 28.339880458524526 28.974838720601433 15 22.38627332498733 32.622547126183775 23.160695180657868 21.830484368171028 16 20.6712045785499 29.02178790341698 29.521402688882116 20.785604829151005 17 20.684090105764 29.454332260242516 28.910484753432513 20.95109288056097 18 20.30697563179856 28.68689818944909 30.929742074011518 20.07638410474083 19 23.1915884388659 27.493359015335116 27.629353681380902 21.68569886441808 20 22.519969352810946 28.623691462387345 28.846984172674052 20.009355012127653 21 21.92506804492023 27.785926236343393 27.543271489707543 22.74573422902883 22 21.57795260258258 29.59269630222256 28.117691381306447 20.711659713888412 23 21.168033068801268 29.014253830141417 28.28108481076556 21.536628290291755 24 22.607767150570293 27.832783394747256 29.04151948137158 20.51792997331087 25 21.665361496782936 28.317812394175412 28.86894683372841 21.14787927531324 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 234.0 1 234.0 2 332.5 3 431.0 4 431.0 5 431.0 6 688.5 7 946.0 8 946.0 9 946.0 10 1445.5 11 1945.0 12 1945.0 13 1945.0 14 3371.5 15 4798.0 16 4798.0 17 4798.0 18 8315.5 19 11833.0 20 11833.0 21 11833.0 22 18896.0 23 25959.0 24 25959.0 25 25959.0 26 37596.0 27 49233.0 28 49233.0 29 49233.0 30 65048.5 31 80864.0 32 80864.0 33 80864.0 34 91410.0 35 101956.0 36 101956.0 37 101956.0 38 108854.5 39 115753.0 40 115753.0 41 115753.0 42 119527.0 43 123301.0 44 123301.0 45 123301.0 46 124713.5 47 126126.0 48 126126.0 49 126126.0 50 118031.5 51 109937.0 52 109937.0 53 109937.0 54 94757.5 55 79578.0 56 79578.0 57 79578.0 58 66378.0 59 53178.0 60 53178.0 61 53178.0 62 42252.5 63 31327.0 64 31327.0 65 31327.0 66 23712.5 67 16098.0 68 16098.0 69 16098.0 70 11612.0 71 7126.0 72 7126.0 73 7126.0 74 4990.5 75 2855.0 76 2855.0 77 2855.0 78 1952.0 79 1049.0 80 1049.0 81 1049.0 82 711.5 83 374.0 84 374.0 85 374.0 86 251.5 87 129.0 88 129.0 89 129.0 90 81.5 91 34.0 92 34.0 93 34.0 94 24.5 95 15.0 96 15.0 97 15.0 98 11.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23225375018384553 2 0.13511983552836934 3 0.13839995682937126 4 0.06856511622739495 5 0.0209504521805929 6 0.019151675983269265 7 0.2908726921436863 8 0.28759257084268436 9 0.017141279056848736 10 0.03449417884279437 11 0.014178588849492163 12 0.015025071765879756 13 0.01343791629765302 14 0.015130882130428205 15 0.013120485204007673 16 0.016823847963203388 17 0.016188985775912695 18 0.013332105933104571 19 0.020844641816044448 20 0.01470764067223441 21 0.013014674839459225 22 0.014919261401331306 23 0.015659933953170446 24 0.014072778484943715 25 0.01343791629765302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 945087.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.75771880042117 #Duplication Level Percentage of deduplicated Percentage of total 1 94.23853833964655 80.81682071094119 2 4.426073190249031 7.5913988007891895 3 0.6369165688654218 1.6386153603626983 4 0.22838335411302563 0.7834254184288746 5 0.10643065965507859 0.456362529122178 6 0.07384797957160608 0.37998205596486256 7 0.05102010053992138 0.30627572046902557 8 0.03629933684697457 0.24903586575716896 9 0.025406891811756708 0.196095337522682 >10 0.1462307251504396 2.3166489240242703 >50 0.016294582717236137 0.9738347014743977 >100 0.011967392832354044 2.1679183497290837 >500 0.0018506271433100325 1.0494669807127281 >1k 7.402508573240131E-4 1.074119244701645 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2482 0.2626213248092504 No Hit CTGTAGGACGTGGAATATGGCAAGA 1837 0.19437363967550078 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1747 0.18485070686614036 No Hit GTCCTACAGTGTGCATTTCTCATTT 1622 0.17162441129758424 No Hit CTTTAGGACGTGAAATATGGCGAGG 1393 0.14739383781598941 No Hit CTGTAGGACCTGGAATATGGCGAGA 1071 0.11332290043138886 No Hit GTATCAACGCAGAGTACTTTTTTTT 946 0.10009660486283273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0581036454844898E-4 0.0 0.0 0.0 6 0.0 1.0581036454844898E-4 0.0 1.0581036454844898E-4 0.0 7 0.0 1.0581036454844898E-4 0.0 1.0581036454844898E-4 0.0 8 0.0 1.0581036454844898E-4 0.0 1.0581036454844898E-4 0.0 9 0.0 1.0581036454844898E-4 0.0 1.0581036454844898E-4 0.0 10 0.0 1.0581036454844898E-4 0.0 1.0581036454844898E-4 0.0 11 0.0 1.0581036454844898E-4 0.0 1.0581036454844898E-4 0.0 12 0.0 1.0581036454844898E-4 0.0 1.0581036454844898E-4 0.0 13 0.0 1.0581036454844898E-4 0.0 1.0581036454844898E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGGTC 35 0.0021235805 16.337776 2 TCGGTCG 35 0.0021235805 16.337776 3 TAGGACC 390 0.0 13.430978 4 ATAGGAC 190 0.0 12.539959 3 GTACTAG 55 0.0029837296 12.135369 1 TGTAGGA 1120 0.0 11.9980545 2 TAATACT 155 2.3646862E-11 11.674316 4 TAGACTC 90 7.277218E-6 11.635852 5 GTCCTAG 165 5.456968E-12 11.557494 1 AGGACCT 755 0.0 11.348633 5 AGGACGT 990 0.0 11.347361 5 CTGTAGG 1120 0.0 11.322734 1 GGACGTG 955 0.0 11.26479 6 GGCGAGG 425 0.0 11.155831 19 GTCCTAC 1265 0.0 11.155496 1 AGGACAG 285 0.0 11.023438 5 TGTAGAA 415 0.0 11.023078 2 GTAGGAC 1125 0.0 11.012871 3 GACGTGG 495 0.0 10.962704 7 TAGGACG 1060 0.0 10.87149 4 >>END_MODULE