FastQCFastQC Report
Thu 26 May 2016
SRR1926800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710979
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12930.18186191153325204No Hit
GTCCTAAAGTGTGTATTTCTCATTT10640.1496528026847488No Hit
CTGTAGGACGTGGAATATGGCAAGA10030.14107308373383742No Hit
GTCCTACAGTGTGCATTTCTCATTT9450.13291531817395452No Hit
CTTTAGGACGTGAAATATGGCGAGG8690.12222583226790101No Hit
GTATCAACGCAGAGTACTTTTTTTT7970.11209895088321878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2950.014.5199534
TGGCGAA400.00532789114.22873718
TTGGGGT707.1039376E-613.5980514
GTTTAGG801.9102263E-613.1131171
AGGACCT4950.012.8756295
GGACCTG4800.012.6834566
GTCCTAG1155.005859E-912.43932151
TGGACTG1251.375156E-912.1761185
GTAAGGC550.002993888712.1291833
ATTATAC802.770126E-511.9125913
CTGGAAT5100.011.53097510
GACCTGG4950.011.5296017
GGCGAGA4650.011.21979319
TTAGACT855.2081392E-511.1983954
CTAGCAC600.005733802411.1184183
ATGGCGA6950.011.05542217
TGGCGAG6450.011.03002818
GTCCTAC7300.010.9738671
GTATAAA1053.3003507E-610.8992151
TAGGCAG700.001473297710.8715345
Produced by FastQC (version 0.11.5)