##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926800_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 710979 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.980086612966065 31.0 27.0 31.0 12.0 33.0 2 27.62857693405853 31.0 28.0 31.0 16.0 34.0 3 27.4348257824774 31.0 28.0 31.0 10.0 34.0 4 31.069374763530288 35.0 32.0 37.0 16.0 37.0 5 30.851949213689856 35.0 32.0 37.0 10.0 37.0 6 30.51706309187754 35.0 31.0 37.0 10.0 37.0 7 30.632714890313217 35.0 32.0 37.0 10.0 37.0 8 30.29380052012788 35.0 30.0 37.0 10.0 37.0 9 32.37848515919598 37.0 32.0 39.0 11.0 39.0 10 32.50117232717141 37.0 32.0 39.0 10.0 39.0 11 32.68773198645811 37.0 33.0 39.0 10.0 39.0 12 32.52576799033445 37.0 33.0 39.0 10.0 39.0 13 32.6571600567668 37.0 33.0 39.0 10.0 39.0 14 33.62092973210179 39.0 33.0 40.0 10.0 41.0 15 33.17724855445801 38.0 32.0 40.0 8.0 41.0 16 33.24723655691659 38.0 33.0 40.0 8.0 41.0 17 33.48029829291723 38.0 33.0 40.0 2.0 41.0 18 33.522506290621806 39.0 33.0 40.0 2.0 41.0 19 32.86123781433769 39.0 32.0 40.0 2.0 41.0 20 33.14582568542812 39.0 32.0 40.0 2.0 41.0 21 33.15854758016763 39.0 33.0 40.0 2.0 41.0 22 33.17577875014592 39.0 33.0 40.0 2.0 41.0 23 33.159180510254174 39.0 33.0 40.0 2.0 41.0 24 31.795452467653757 38.0 31.0 40.0 2.0 41.0 25 32.386801860533154 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.36943400428746287 1101 2 -0.3314442469578438 1101 3 -1.0550124563872636 1101 4 -0.3070885335738964 1101 5 -0.29733286967741535 1101 6 -2.2099586033375296 1101 7 -0.6696698349819208 1101 8 0.09218822896844259 1101 9 -0.2921311136686242 1101 10 -8.772844090616207 1101 11 -4.156685567367134 1101 12 -1.8263390446156187 1101 13 -1.0751341514603894 1101 14 -0.7471829789626554 1101 15 -1.8896696553530745 1101 16 -0.34262919809766146 1101 17 -0.272790731705264 1101 18 -0.13328066465314237 1101 19 -0.8407487567579395 1101 20 -0.08480742774985117 1101 21 -0.2768505395580263 1101 22 -0.11923179673726025 1101 23 -0.22916388421341338 1101 24 -0.38518858898566677 1101 25 -0.3288124327932209 1102 1 -0.9291866508129836 1102 2 -0.6214746120050414 1102 3 -0.9570943453531839 1102 4 -0.8547059939760082 1102 5 -0.965547231578114 1102 6 -1.7023948400715696 1102 7 -0.6220940390243719 1102 8 -0.17674659450764096 1102 9 -0.31151424656820836 1102 10 -0.4000556393336083 1102 11 -0.510924174641417 1102 12 -0.6156027838828599 1102 13 -0.834078674419164 1102 14 -0.8960167078304551 1102 15 -0.3528941167151345 1102 16 -0.5720452959057809 1102 17 -0.7740173329531927 1102 18 -1.0833941586001359 1102 19 -0.3972590762075683 1102 20 -0.587746437669324 1102 21 -0.6126776287799345 1102 22 -0.8126781462652843 1102 23 -1.3902777914716928 1102 24 -4.537977106931379 1102 25 -3.0454617114983584 1103 1 -0.3020420429698447 1103 2 -0.1521093487764631 1103 3 0.14950987167609142 1103 4 -0.04775595368592178 1103 5 -0.375636541274595 1103 6 0.42041186822818943 1103 7 0.3479922489154603 1103 8 0.40805984519152716 1103 9 0.42520765109497205 1103 10 0.32820617909239047 1103 11 -0.16668817544721293 1103 12 -0.4505552751533415 1103 13 -0.005353519973823495 1103 14 -0.08145687704882221 1103 15 0.10497839872940062 1103 16 0.26937459396797436 1103 17 0.05910111990740319 1103 18 -0.1891581594059346 1103 19 -2.0368440882945933 1103 20 -0.7866997347415747 1103 21 -0.3638544351189097 1103 22 -0.4623524680487918 1103 23 -0.3430351444128732 1103 24 1.2870262505760088 1103 25 0.7005504091033075 1104 1 -0.6251003687480257 1104 2 -0.3758685443286538 1104 3 -1.3804884310019503 1104 4 -0.41769596784406815 1104 5 -0.368049098145228 1104 6 -1.934346635975789 1104 7 -0.1674032891976971 1104 8 0.047763931597636144 1104 9 0.1113140359233924 1104 10 0.35952218318252704 1104 11 0.1035138886618796 1104 12 0.42660746490387425 1104 13 -0.131344486909164 1104 14 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0.4335489832539565 2215 3 0.6752037231279395 2215 4 1.1451842606333003 2215 5 1.0607367894930029 2215 6 1.5888557086553874 2215 7 0.9290747493865155 2215 8 -0.2624714628131706 2215 9 0.3714450378918457 2215 10 0.12581683652240372 2215 11 0.07052062342706478 2215 12 0.04400816870712987 2215 13 0.1875137311998003 2215 14 0.3859466372090594 2215 15 0.4723775031268289 2215 16 0.653728409098612 2215 17 0.18691330795988392 2215 18 -0.2875068671163774 2215 19 0.7665907920979649 2215 20 -3.251555151453342 2215 21 -1.4597199905007443 2215 22 -0.44958552115554085 2215 23 -0.09825902500988803 2215 24 0.024083911505847766 2215 25 0.183316273576267 2216 1 -0.6329346510591058 2216 2 -1.4699586447394744 2216 3 -0.815219499180408 2216 4 -1.1233190877490813 2216 5 -1.0261705394786809 2216 6 -2.4940089491043054 2216 7 -10.143652924084673 2216 8 -11.01983129177567 2216 9 -6.46793730219213 2216 10 -4.03872318794301 2216 11 -2.99939867008813 2216 12 -2.739775962568558 2216 13 -2.3177806803261305 2216 14 -2.531623094736677 2216 15 -3.1613061583100155 2216 16 -2.429162881433715 2216 17 -2.79567620570732 2216 18 -2.3982284866828287 2216 19 -1.9170412568622552 2216 20 -2.7774192471696892 2216 21 -5.670493589892416 2216 22 -3.9391087394230517 2216 23 -3.758097905919147 2216 24 -4.030055005036228 2216 25 -3.22337442993301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50939.0 3 4.0 4 418.0 5 2280.0 6 2753.0 7 2247.0 8 1794.0 9 1763.0 10 1594.0 11 1469.0 12 1455.0 13 1455.0 14 1389.0 15 1558.0 16 1584.0 17 1753.0 18 2055.0 19 2493.0 20 3257.0 21 4192.0 22 4981.0 23 6120.0 24 7148.0 25 7861.0 26 9073.0 27 9939.0 28 11087.0 29 13156.0 30 15649.0 31 19097.0 32 24116.0 33 31936.0 34 42292.0 35 58305.0 36 83531.0 37 130522.0 38 149714.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5663705589122 19.022351132214016 12.704446126287634 25.706832182586158 2 13.89909464858311 20.527635820315414 42.50265099399953 23.070618537101947 3 18.440405474297332 24.921347112023682 30.319878549237977 26.318368864441013 4 11.253392034770467 17.231677382306245 39.6375448987175 31.877385684205784 5 12.544192727421086 38.17576360390373 36.87034595888898 12.409697709786201 6 31.033856958360417 38.02570011282839 18.99667142177841 11.943771507032782 7 26.84841359126397 31.405479475229015 23.990537656237223 17.755569277269792 8 23.777461993052736 37.76771213966707 21.70526571029007 16.74956015699012 9 25.809260671267836 15.67187275791535 20.735361561208386 37.78350500960843 10 13.501709558047587 30.03996003996004 35.95953342432215 20.498796977670217 11 33.57107587644703 23.17328617871768 25.23841075440351 18.01722719043178 12 22.406431781893378 25.510061968502274 32.86609598435665 19.217410265247697 13 28.354748768389747 22.868843003806667 26.174707184838603 22.60170104296498 14 19.643578117812233 23.10974434579788 28.670325233560717 28.57635230282918 15 22.363364225144473 32.08457829020296 23.53404570187433 22.01801178277824 16 20.743051042027165 29.156361753790662 29.187733706983142 20.912853497199034 17 20.696936658108537 29.563253965462664 28.73041887947104 21.00939049695776 18 20.384065137177174 28.61596159067282 30.654874526811547 20.345098745338454 19 23.112809373206225 27.67054215579241 27.871444810858875 21.345203660142488 20 22.520226042110682 28.505812129576093 28.870028599926002 20.10393322838722 21 22.135097951501134 27.775995903564965 27.790344680120754 22.298561464813144 22 21.72577221258583 29.336129997031712 28.13812254957818 20.79997524080428 23 21.058281000292613 28.87475521642245 28.628986877349362 21.437976905935578 24 22.64816891065905 27.724688792886372 28.960936104245416 20.666206192209167 25 21.67939296405501 28.353946746494408 28.73137477773526 21.235285511715322 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 246.0 2 291.0 3 336.0 4 336.0 5 336.0 6 557.0 7 778.0 8 778.0 9 778.0 10 1191.5 11 1605.0 12 1605.0 13 1605.0 14 2813.0 15 4021.0 16 4021.0 17 4021.0 18 6907.5 19 9794.0 20 9794.0 21 9794.0 22 15425.0 23 21056.0 24 21056.0 25 21056.0 26 29565.5 27 38075.0 28 38075.0 29 38075.0 30 49310.5 31 60546.0 32 60546.0 33 60546.0 34 68805.0 35 77064.0 36 77064.0 37 77064.0 38 82429.0 39 87794.0 40 87794.0 41 87794.0 42 90623.5 43 93453.0 44 93453.0 45 93453.0 46 93202.0 47 92951.0 48 92951.0 49 92951.0 50 87050.0 51 81149.0 52 81149.0 53 81149.0 54 70047.0 55 58945.0 56 58945.0 57 58945.0 58 49174.5 59 39404.0 60 39404.0 61 39404.0 62 31338.5 63 23273.0 64 23273.0 65 23273.0 66 17688.0 67 12103.0 68 12103.0 69 12103.0 70 8659.0 71 5215.0 72 5215.0 73 5215.0 74 3641.0 75 2067.0 76 2067.0 77 2067.0 78 1399.0 79 731.0 80 731.0 81 731.0 82 497.0 83 263.0 84 263.0 85 263.0 86 169.5 87 76.0 88 76.0 89 76.0 90 49.5 91 23.0 92 23.0 93 23.0 94 13.0 95 3.0 96 3.0 97 3.0 98 5.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20253762769364497 2 0.12208518113755822 3 0.12616406391749965 4 0.06905970499831922 5 0.024191994418963147 6 0.023207436506563484 7 0.23080850489254956 8 0.22996459811049272 9 0.020394413899707305 10 0.037835154062215624 11 0.01772204242319393 12 0.018847251465936406 13 0.017018786771479888 14 0.019128553726622026 15 0.015612275468051799 16 0.021519622942449777 17 0.021660274072792588 18 0.01547162433770899 19 0.02630176137410528 20 0.019409855987307642 21 0.016456182250108652 22 0.01856594920525079 23 0.02067571616039292 24 0.01659683338045146 25 0.016174879989423036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 710979.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.70793377082693 #Duplication Level Percentage of deduplicated Percentage of total 1 95.2384526804309 84.48406352811698 2 3.7071076348861483 6.576997171136146 3 0.495916413221955 1.3197516101987765 4 0.1752440063252008 0.6218213482732114 5 0.0912441613330412 0.4047040510253033 6 0.05958341609245894 0.3171313037141683 7 0.03921032259237628 0.2434786689760092 8 0.032908802323859974 0.2335417485617765 9 0.022488955314266704 0.17954538827337513 >10 0.11345731546101921 1.9131269866699372 >50 0.012668555535977545 0.7679697919354338 >100 0.010292606377231804 1.8623121841467982 >500 9.500867369926388E-4 0.6035869234586941 >1k 4.750433684963194E-4 0.47196929551343075 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1293 0.18186191153325204 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1064 0.1496528026847488 No Hit CTGTAGGACGTGGAATATGGCAAGA 1003 0.14107308373383742 No Hit GTCCTACAGTGTGCATTTCTCATTT 945 0.13291531817395452 No Hit CTTTAGGACGTGAAATATGGCGAGG 869 0.12222583226790101 No Hit GTATCAACGCAGAGTACTTTTTTTT 797 0.11209895088321878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 295 0.0 14.519953 4 TGGCGAA 40 0.005327891 14.228737 18 TTGGGGT 70 7.1039376E-6 13.598051 4 GTTTAGG 80 1.9102263E-6 13.113117 1 AGGACCT 495 0.0 12.875629 5 GGACCTG 480 0.0 12.683456 6 GTCCTAG 115 5.005859E-9 12.4393215 1 TGGACTG 125 1.375156E-9 12.176118 5 GTAAGGC 55 0.0029938887 12.129183 3 ATTATAC 80 2.770126E-5 11.912591 3 CTGGAAT 510 0.0 11.530975 10 GACCTGG 495 0.0 11.529601 7 GGCGAGA 465 0.0 11.219793 19 TTAGACT 85 5.2081392E-5 11.198395 4 CTAGCAC 60 0.0057338024 11.118418 3 ATGGCGA 695 0.0 11.055422 17 TGGCGAG 645 0.0 11.030028 18 GTCCTAC 730 0.0 10.973867 1 GTATAAA 105 3.3003507E-6 10.899215 1 TAGGCAG 70 0.0014732977 10.871534 5 >>END_MODULE