FastQCFastQC Report
Thu 26 May 2016
SRR1926800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710979
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14800.20816367290735732No Hit
GTCCTACAGTGGACATTTCTAAATT13390.18833186352902126No Hit
CTGTAGGACGTGGAATATGGCAAGA12670.178204982144339No Hit
GTCCTAAAGTGTGTATTTCTCATTT10920.15359103433434743No Hit
CTTTAGGACGTGAAATATGGCGAGG10840.15246582529160496No Hit
GTCCTACAGTGTGCATTTCTCATTT9860.13868201451800966No Hit
GGTATCAACGCAGAGTACTTTTTTT8820.12405429696235754No Hit
TATCAACGCAGAGTACTTTTTTTTT7590.10675420793019204No Hit
CTGTAGGACCTGGAATATGGCGAGA7400.10408183645367866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA651.939552E-716.05448719
GTCTTAG1206.9485395E-1012.714271
TAGGACC3000.012.3841554
TCAATAC657.82577E-411.7254723
GGCGAGG2450.011.61641919
GTATAGG600.005708646511.1249861
CCTCTAA600.005708646511.1249861
ATCCCGT600.00593478511.06708710
AGGACGT6500.010.9918545
ATTACAC700.001454877210.88793853
TTGACCT700.001455735110.8871694
AATGCCG700.00150713410.8419918
TACACTG4500.010.7964445
TCAGAAC1158.474108E-710.769593
GATATAC2400.010.7276651
GGATATA803.6369168E-410.7276641
CCCTGTA1252.1215055E-710.6792312
TAGGACG6700.010.6637384
GGACGTG6350.010.6514236
GGACCTG5650.010.6222166
Produced by FastQC (version 0.11.5)