##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926800_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 710979 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.617307965495463 31.0 30.0 33.0 25.0 34.0 2 29.984715441665646 31.0 30.0 33.0 26.0 34.0 3 30.06022259447888 31.0 30.0 34.0 26.0 34.0 4 33.63205383000061 35.0 35.0 37.0 28.0 37.0 5 33.57864156325292 37.0 35.0 37.0 28.0 37.0 6 33.54905700449662 37.0 35.0 37.0 28.0 37.0 7 33.536472947864844 37.0 35.0 37.0 28.0 37.0 8 33.546611081339954 37.0 35.0 37.0 28.0 37.0 9 35.06007913032593 39.0 35.0 39.0 28.0 39.0 10 34.940597401611015 39.0 35.0 39.0 28.0 39.0 11 34.944255737511234 39.0 35.0 39.0 28.0 39.0 12 34.85165243980483 39.0 35.0 39.0 27.0 39.0 13 34.8550041562409 39.0 35.0 39.0 27.0 39.0 14 35.937306165161 40.0 36.0 41.0 27.0 41.0 15 35.92515109447677 40.0 36.0 41.0 27.0 41.0 16 35.903566772014365 40.0 36.0 41.0 27.0 41.0 17 35.88192618909982 40.0 36.0 41.0 27.0 41.0 18 35.855243263162485 40.0 36.0 41.0 27.0 41.0 19 35.9297574189955 40.0 36.0 41.0 27.0 41.0 20 35.88767600730823 40.0 36.0 41.0 27.0 41.0 21 35.830945780395766 40.0 36.0 41.0 27.0 41.0 22 35.73966319680328 40.0 36.0 41.0 27.0 41.0 23 35.66629956721647 40.0 36.0 41.0 27.0 41.0 24 35.60128639523811 40.0 36.0 41.0 27.0 41.0 25 35.480481139386676 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1355937335947317 1101 2 -0.07174409084701239 1101 3 -0.012818615180339776 1101 4 0.06281625753113929 1101 5 0.1334543581264569 1101 6 0.2141849973669281 1101 7 -0.060582750247462513 1101 8 0.12235184464807247 1101 9 0.13674618299310026 1101 10 0.18985617540596422 1101 11 0.22386381260356103 1101 12 0.24074870145264526 1101 13 0.2734492960530446 1101 14 0.21265276220913165 1101 15 0.368270833115254 1101 16 0.3964892539257505 1101 17 0.22703739544425616 1101 18 0.27983425050055644 1101 19 0.22680281840619898 1101 20 0.40091442757836404 1101 21 0.23834774320653906 1101 22 0.33133700052091797 1101 23 0.29538463592108855 1101 24 0.33443413550541834 1101 25 0.35423023100059225 1102 1 -0.480559502087079 1102 2 -0.343652089087108 1102 3 -0.15882082839563694 1102 4 -0.22815405738540306 1102 5 -0.2479586331806729 1102 6 -0.2842625854286567 1102 7 -0.17765733960269614 1102 8 -0.18599421022899776 1102 9 -0.22643778052884755 1102 10 -0.15240640392313765 1102 11 -0.17093263158608352 1102 12 -0.14109843336683525 1102 13 -0.17916989758996493 1102 14 -0.07263702233569092 1102 15 -0.20055216299162026 1102 16 -0.26508962853385043 1102 17 -0.0923722360420598 1102 18 -0.22578128333763914 1102 19 -0.38583700746976035 1102 20 -0.23495415943033038 1102 21 -0.12181554808459083 1102 22 -0.17264612037930505 1102 23 -0.10043592031590975 1102 24 -0.006651839909224577 1102 25 -0.11847039163286865 1103 1 -0.6054008598630674 1103 2 -0.753003586535403 1103 3 -0.5497320559003356 1103 4 -0.5563378137646637 1103 5 -0.6169151198849434 1103 6 -0.549558549704777 1103 7 -0.8436583804299858 1103 8 -0.7533768653458424 1103 9 -0.7294427496397802 1103 10 -0.6330998970738193 1103 11 -0.7100971158729479 1103 12 -0.7457108091390623 1103 13 -0.6582156939907122 1103 14 -0.7418167832811662 1103 15 -0.8899961597684083 1103 16 -0.7950713301185957 1103 17 -0.6361675623917762 1103 18 -0.795957384600058 1103 19 -0.7281692999758036 1103 20 -0.7194265271445346 1103 21 -0.754703172312368 1103 22 -0.7194799909138183 1103 23 -0.8674903790857229 1103 24 -0.6770612879350111 1103 25 -0.769263602752936 1104 1 -0.17185846614233213 1104 2 -0.18325814343087643 1104 3 -0.0010325771023715902 1104 4 -0.036005138661067804 1104 5 -0.007071480495483229 1104 6 -0.043294603721008684 1104 7 -0.09412762785399309 1104 8 -0.07438432942264228 1104 9 -0.02191202190264363 1104 10 -0.025918983252239514 1104 11 0.16040053064526916 1104 12 -0.06931476182931817 1104 13 -0.04296049542474378 1104 14 -0.17537987605015815 1104 15 -0.04514711792735682 1104 16 -0.05681990291921579 1104 17 0.08832479344970068 1104 18 0.005128901452508217 1104 19 -0.16304305648047546 1104 20 0.025574445710731197 1104 21 -0.20861508544260232 1104 22 -0.14826408742106167 1104 23 7.336839718590227E-4 1104 24 -0.0028278771872365382 1104 25 -0.07641346800213 1105 1 0.4326734901259037 1105 2 0.23580143874620774 1105 3 0.47810303775189666 1105 4 0.5186084781296998 1105 5 0.3955092190765086 1105 6 0.1518513391214853 1105 7 0.020255490556195355 1105 8 -0.12766559031949498 1105 9 0.09036349275327638 1105 10 0.32078791629152903 1105 11 0.18230614827291447 1105 12 0.38247471458644355 1105 13 0.5296654701886325 1105 14 0.610144073107854 1105 15 0.24204491161640362 1105 16 0.3265409749597836 1105 17 0.4922589330624305 1105 18 0.7334207449692158 1105 19 0.7085131319598972 1105 20 0.5349260757585625 1105 21 0.5020844703306437 1105 22 0.5919216476939368 1105 23 0.3254854992358105 1105 24 0.5626108271432813 1105 25 0.4698816419354799 1106 1 0.43992154410497974 1106 2 0.17284165463679813 1106 3 0.3150342796853316 1106 4 0.41566184161570874 1106 5 0.35206911376715766 1106 6 0.5096692561759113 1106 7 0.4628536997186927 1106 8 0.3134153413274703 1106 9 0.27519730746861626 1106 10 0.3524088476491869 1106 11 0.4443121691644052 1106 12 0.1913025694871422 1106 13 0.4620984021827752 1106 14 0.5099844696573328 1106 15 0.3083004346512155 1106 16 0.24931813929025992 1106 17 0.19110908101306023 1106 18 0.15740819528399896 1106 19 0.35741206102556333 1106 20 0.3921243842512965 1106 21 0.5197838982977316 1106 22 0.3542981478940419 1106 23 0.27061839763768347 1106 24 0.3782206232269729 1106 25 0.5415906846844507 1107 1 0.34599983413637503 1107 2 0.20687179985946713 1107 3 0.4238105251166431 1107 4 0.31184662404339036 1107 5 0.49705071212849816 1107 6 0.6225009198410589 1107 7 0.4440547664665573 1107 8 0.4788928997341415 1107 9 0.3285275639496348 1107 10 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9 -0.03505959208533227 1110 10 -0.18794290834791383 1110 11 -0.2420040136787378 1110 12 -0.11658808719296587 1110 13 -0.07592289082265324 1110 14 -0.22530165013370862 1110 15 -0.11793894071155364 1110 16 -0.17392129173010318 1110 17 -0.01514683260634797 1110 18 -0.1691945446598666 1110 19 -0.16382210637657835 1110 20 -0.03582447437046454 1110 21 -0.1962152747894379 1110 22 -0.31397225260210604 1110 23 -0.11419503015454069 1110 24 -0.23283774568746196 1110 25 -0.18193180495926242 1111 1 0.42277161287208287 1111 2 0.42938400364523943 1111 3 0.47457237950263575 1111 4 0.4731940895183513 1111 5 0.5680886117278305 1111 6 0.614061034441086 1111 7 0.4864705808976595 1111 8 0.28033765423910495 1111 9 0.09749869212699735 1111 10 0.2990160477464556 1111 11 0.367323015576126 1111 12 0.17781600631062133 1111 13 0.1542775850937943 1111 14 0.05997760084223103 1111 15 0.17704527317089713 1111 16 0.1514935798813255 1111 17 0.1396395493399325 1111 18 0.06418337343895786 1111 19 0.19190677677504908 1111 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0.26960612104215187 2211 10 0.24013627109847135 2211 11 0.2671989142474658 2211 12 0.239382113432697 2211 13 0.15453733206075526 2211 14 0.1782733569413466 2211 15 0.21456807697990854 2211 16 0.12153002040610517 2211 17 0.23193250746294325 2211 18 0.17715051924511016 2211 19 0.12670573110363392 2211 20 0.11826108410009795 2211 21 0.032546169592968965 2211 22 -9.006071351151945E-4 2211 23 0.019109747994626503 2211 24 0.03449078935509675 2211 25 0.0856682592899034 2212 1 0.39757410716755004 2212 2 0.23885406493564787 2212 3 0.2573473517992504 2212 4 0.4197856204371604 2212 5 0.4383902775891855 2212 6 0.35587615111540316 2212 7 0.33223931745561686 2212 8 0.26568953677370644 2212 9 0.2715948453575834 2212 10 0.41934934195717233 2212 11 0.3355681528126766 2212 12 0.3715659396243396 2212 13 0.3921242965838019 2212 14 0.30688602495781225 2212 15 0.2297320061834256 2212 16 0.29500854885505845 2212 17 0.2374961380204894 2212 18 0.20932167697051085 2212 19 0.25528541613108047 2212 20 0.08181937890955027 2212 21 0.05464627146542966 2212 22 0.08136196594282552 2212 23 0.3753290279010173 2212 24 0.17283119029331573 2212 25 0.3175094372023963 2213 1 0.03410383595555899 2213 2 0.08995069154479651 2213 3 0.21126971874024747 2213 4 0.3009053119955354 2213 5 0.22597535089330734 2213 6 0.12429042638742516 2213 7 0.2350120349156697 2213 8 0.22745701633149196 2213 9 0.2773607039304409 2213 10 0.08633540830393827 2213 11 0.1654427537208818 2213 12 0.16446844949985007 2213 13 0.14738738471160673 2213 14 -0.02183911583684761 2213 15 -0.015604821609649377 2213 16 -0.02776060849788564 2213 17 -0.11981893524576037 2213 18 -0.037452070648193114 2213 19 0.08282549574113318 2213 20 0.08328129523188466 2213 21 0.16568525463824102 2213 22 0.05197820533351205 2213 23 0.08562720580790284 2213 24 -0.009597029993990702 2213 25 0.04456662994432037 2214 1 0.38897985777451183 2214 2 0.3404417690443786 2214 3 0.4737483757566139 2214 4 0.277955371680342 2214 5 0.35455001733835445 2214 6 0.33808819311806104 2214 7 0.3542955150685785 2214 8 0.33460483821390596 2214 9 0.1954912852150983 2214 10 -0.03827361845601018 2214 11 -0.013763513939018424 2214 12 0.17978175694458542 2214 13 0.05158269756574896 2214 14 0.14632924064008535 2214 15 -0.13740337406428438 2214 16 -0.0826177427207 2214 17 0.1031294940617471 2214 18 0.3181741664893565 2214 19 0.21845581259944424 2214 20 0.18400129039665813 2214 21 0.06617104361090753 2214 22 0.22462896612588423 2214 23 0.14599574058374287 2214 24 -0.08001905444209712 2214 25 -0.06435058194428933 2215 1 0.3920398174914972 2215 2 0.2315097853641639 2215 3 0.32400401796081724 2215 4 0.44418736052504926 2215 5 0.5328798216042259 2215 6 0.4805118877934689 2215 7 0.27401353397061 2215 8 0.1979911936340386 2215 9 0.25978917293910797 2215 10 0.22332065929020928 2215 11 0.07556923408556315 2215 12 -0.025028964534520526 2215 13 0.07878664323532547 2215 14 0.15922755185219728 2215 15 0.0400097334087306 2215 16 0.1242458631255019 2215 17 0.03515190794389156 2215 18 -0.06567647648952146 2215 19 -0.09003202502901786 2215 20 -0.12500299612919008 2215 21 -0.17143418285193235 2215 22 -0.14767904399651144 2215 23 -0.11061480530274537 2215 24 -0.16895155457120836 2215 25 -0.08166453122900919 2216 1 -0.9489441624365753 2216 2 -0.7359614971599591 2216 3 -2.337644973116287 2216 4 -2.3050099828099633 2216 5 -1.3600371465944079 2216 6 -1.220174319369093 2216 7 -1.0899719778010777 2216 8 -0.9474274184495286 2216 9 -0.9783298553504096 2216 10 -1.124098349529831 2216 11 -1.1870960268605089 2216 12 -1.0341539034628227 2216 13 -1.1991520536322966 2216 14 -1.4717340565468646 2216 15 -1.2547608757654274 2216 16 -1.111689277807784 2216 17 -1.144796553049197 2216 18 -1.1495329754370687 2216 19 -1.0441290638320524 2216 20 -0.9914341311261197 2216 21 -1.1432475256873502 2216 22 -1.072919932833159 2216 23 -1.2165370155657271 2216 24 -1.3954770614204222 2216 25 -1.2299989727305771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19397.0 3 21.0 4 321.0 5 2096.0 6 2833.0 7 2096.0 8 1596.0 9 1338.0 10 1228.0 11 1068.0 12 1057.0 13 969.0 14 994.0 15 980.0 16 977.0 17 1067.0 18 1100.0 19 1344.0 20 1783.0 21 2290.0 22 2850.0 23 3576.0 24 4224.0 25 4852.0 26 5912.0 27 6671.0 28 7591.0 29 9263.0 30 11579.0 31 15063.0 32 19688.0 33 25403.0 34 34895.0 35 48894.0 36 71022.0 37 122515.0 38 272426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.04150180375282 20.074881791530668 10.327716964288228 28.555899440428284 2 13.768378134472403 21.633649496611934 40.27048221352777 24.327490155387892 3 17.972101947743198 25.6468797564688 29.37552927804365 27.005489017744356 4 11.611594716580939 18.29364861690711 38.544035759143384 31.550720907368575 5 12.349249305850547 39.058038489778355 35.99323151536975 12.599480689001352 6 30.560667991508968 39.16461541935775 18.135331511675172 12.139385077458108 7 27.211685362909694 32.29723228077284 22.39891508332419 18.092167272993283 8 24.033179868704046 39.02348107293016 20.35242005702526 16.590919001340527 9 25.840819002017124 15.807678565902487 20.228466453044952 38.12303597903544 10 13.639208769198094 30.665890233112926 34.974533823386686 20.7203671743023 11 33.649253101527556 23.367513432919797 24.8336005851408 18.149632880411847 12 22.585617859317928 25.714120951533026 32.40630173324388 19.293959455905167 13 28.56246703470586 22.946798410633285 25.885298357888814 22.60543619677204 14 19.836872819028407 23.380718699145824 28.131351277604544 28.65105720422122 15 21.995127728345537 32.22521034879502 23.651053080192753 22.128608842666697 16 20.923549946974152 29.4889827194806 28.60048328068256 20.98698405286269 17 20.608062966698032 29.86867403959892 28.329388069127216 21.19387492457583 18 20.358886433738363 28.93834821660898 30.406187541931732 20.296577807720926 19 22.421509526314455 27.98173783155034 27.991583424522275 21.60516921761292 20 22.410351981433944 28.651640353036324 28.810295720665284 20.127711944864444 21 21.854387546825304 27.738585738661698 27.85112120491724 22.555905509595757 22 21.906132248631817 29.420137584935702 27.80391544616648 20.869814720266 23 21.179106555889838 28.98693210347985 28.29113096167397 21.54283037895634 24 22.03668749243999 28.023229973360635 29.15589511911761 20.78418741508176 25 21.692236064596077 28.523604631045334 28.53710786127119 21.2470514430874 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 258.0 1 258.0 2 258.0 3 258.0 4 258.0 5 258.0 6 452.0 7 646.0 8 646.0 9 646.0 10 1087.0 11 1528.0 12 1528.0 13 1528.0 14 2578.0 15 3628.0 16 3628.0 17 3628.0 18 6426.0 19 9224.0 20 9224.0 21 9224.0 22 14668.0 23 20112.0 24 20112.0 25 20112.0 26 28615.0 27 37118.0 28 37118.0 29 37118.0 30 48448.0 31 59778.0 32 59778.0 33 59778.0 34 67864.0 35 75950.0 36 75950.0 37 75950.0 38 81659.0 39 87368.0 40 87368.0 41 87368.0 42 89988.0 43 92608.0 44 92608.0 45 92608.0 46 93772.5 47 94937.0 48 94937.0 49 94937.0 50 89360.0 51 83783.0 52 83783.0 53 83783.0 54 71979.5 55 60176.0 56 60176.0 57 60176.0 58 50089.5 59 40003.0 60 40003.0 61 40003.0 62 31739.0 63 23475.0 64 23475.0 65 23475.0 66 17762.0 67 12049.0 68 12049.0 69 12049.0 70 8615.0 71 5181.0 72 5181.0 73 5181.0 74 3548.5 75 1916.0 76 1916.0 77 1916.0 78 1314.5 79 713.0 80 713.0 81 713.0 82 456.5 83 200.0 84 200.0 85 200.0 86 128.5 87 57.0 88 57.0 89 57.0 90 34.0 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11153634636184753 2 0.09733058219722382 3 0.014768368685994944 4 0.0 5 0.005204091822683932 6 0.3469863385557098 7 0.01983180937833607 8 0.009282974602625395 9 0.009142323472282585 10 0.0050634406923411234 11 0.005485394083369551 12 0.0 13 5.62604521371236E-4 14 0.0 15 0.0023910692158277532 16 7.03255651714045E-4 17 0.0 18 2.81302260685618E-4 19 1.40651130342809E-4 20 5.62604521371236E-4 21 0.012939903991538427 22 0.0 23 0.0 24 1.40651130342809E-4 25 0.005344742953026742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 710979.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.03910449123421 #Duplication Level Percentage of deduplicated Percentage of total 1 93.71257679797456 80.62946187263745 2 4.934314588808888 8.490880169982987 3 0.6710172461258759 1.7320116886453338 4 0.22985777042738698 0.7910702691169629 5 0.11385325644332751 0.48979161138973704 6 0.06716459445387916 0.34672689361972076 7 0.04944647481011378 0.2978031289037392 8 0.03192817152718296 0.21976570289930752 9 0.02581083937906564 0.1998667355707702 >10 0.13585875839710407 2.2158281390836434 >50 0.014413590189062955 0.8878147747720124 >100 0.011464926230373316 1.945807546528075 >500 0.0014740619356327898 0.8707376009700221 >1k 8.189232975737722E-4 0.8824338658802845 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1480 0.20816367290735732 No Hit GTCCTACAGTGGACATTTCTAAATT 1339 0.18833186352902126 No Hit CTGTAGGACGTGGAATATGGCAAGA 1267 0.178204982144339 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1092 0.15359103433434743 No Hit CTTTAGGACGTGAAATATGGCGAGG 1084 0.15246582529160496 No Hit GTCCTACAGTGTGCATTTCTCATTT 986 0.13868201451800966 No Hit GGTATCAACGCAGAGTACTTTTTTT 882 0.12405429696235754 No Hit TATCAACGCAGAGTACTTTTTTTTT 759 0.10675420793019204 No Hit CTGTAGGACCTGGAATATGGCGAGA 740 0.10408183645367866 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 65 1.939552E-7 16.054487 19 GTCTTAG 120 6.9485395E-10 12.71427 1 TAGGACC 300 0.0 12.384155 4 TCAATAC 65 7.82577E-4 11.725472 3 GGCGAGG 245 0.0 11.616419 19 GTATAGG 60 0.0057086465 11.124986 1 CCTCTAA 60 0.0057086465 11.124986 1 ATCCCGT 60 0.005934785 11.067087 10 AGGACGT 650 0.0 10.991854 5 ATTACAC 70 0.0014548772 10.8879385 3 TTGACCT 70 0.0014557351 10.887169 4 AATGCCG 70 0.001507134 10.84199 18 TACACTG 450 0.0 10.796444 5 TCAGAAC 115 8.474108E-7 10.76959 3 GATATAC 240 0.0 10.727665 1 GGATATA 80 3.6369168E-4 10.727664 1 CCCTGTA 125 2.1215055E-7 10.679231 2 TAGGACG 670 0.0 10.663738 4 GGACGTG 635 0.0 10.651423 6 GGACCTG 565 0.0 10.622216 6 >>END_MODULE