FastQCFastQC Report
Thu 26 May 2016
SRR1926799_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926799_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609452
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12520.20543045227515866No Hit
GTACATGGGAAGCAGTGGTATCAAC10580.1735985770823625No Hit
CCCATGTACTCTGCGTTGATACCAC9430.15472916652993182No Hit
GGTATCAACGCAGAGTACTTTTTTT7350.12060014570466583No Hit
TATCAACGCAGAGTACTTTTTTTTT6920.11354462697636565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGA953.1286618E-1014.97065719
TAGGACC1207.0940587E-1012.6960544
TATGGCG1850.011.78770716
CAGGACT752.0257676E-411.4264514
TAGGACA752.0257676E-411.4264514
TGGCGAG1850.011.27519818
TTACACT1104.8024594E-711.2533224
GTATAAA855.0571536E-511.2301641
ATTACAC855.1084822E-511.2190423
GTGTTAC600.00566295211.1365781
GGATTGG600.00566295211.1365781
CTTACTC600.005704890511.1255493
GTGTAGT1053.2614334E-610.9093011
CCTGGAC1158.361385E-710.7800363
CTACAGT1951.8189894E-1210.7428164
TGCGTTG2300.010.718122512
TAGGACT803.6837978E-410.71229654
GTAGGAC2050.010.6991363
AACAGGG909.318412E-510.5748187
TCCTACA2100.010.4443942
Produced by FastQC (version 0.11.5)