##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926799_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609452 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.890864251819668 31.0 27.0 31.0 10.0 33.0 2 27.53462947040948 31.0 28.0 31.0 15.0 34.0 3 27.365633388683605 31.0 27.0 31.0 10.0 34.0 4 30.979322407671155 35.0 32.0 37.0 12.0 37.0 5 30.817879012621173 35.0 32.0 37.0 10.0 37.0 6 30.451758300899826 35.0 31.0 37.0 10.0 37.0 7 30.484317058603466 35.0 31.0 37.0 10.0 37.0 8 30.16334674428831 35.0 30.0 37.0 10.0 37.0 9 32.24297237518295 37.0 32.0 39.0 10.0 39.0 10 32.38150174254904 37.0 32.0 39.0 10.0 39.0 11 32.54721946929373 37.0 33.0 39.0 10.0 39.0 12 32.38128515453227 37.0 32.0 39.0 8.0 39.0 13 32.505157091944895 37.0 33.0 39.0 8.0 39.0 14 33.486555134776815 39.0 33.0 40.0 2.0 41.0 15 33.0008466622474 38.0 32.0 40.0 2.0 41.0 16 33.07767797956197 38.0 32.0 40.0 2.0 41.0 17 33.32306563929563 39.0 33.0 40.0 2.0 41.0 18 33.37771965634701 39.0 33.0 40.0 2.0 41.0 19 32.73117817317853 39.0 32.0 40.0 2.0 41.0 20 32.99187302691598 39.0 32.0 40.0 2.0 41.0 21 32.9899040449453 39.0 33.0 40.0 2.0 41.0 22 32.99809336912505 39.0 33.0 40.0 2.0 41.0 23 32.989675971200356 39.0 33.0 40.0 2.0 41.0 24 31.66111194975158 38.0 31.0 40.0 2.0 41.0 25 32.230912360612486 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4792594505578833 1101 2 -0.3219184274637996 1101 3 -1.0888474285568535 1101 4 -0.41409531388188014 1101 5 -0.3166908284240151 1101 6 -2.1265361272823 1101 7 -0.5918903425359936 1101 8 -0.10181365485018645 1101 9 -0.14227891253545266 1101 10 -8.750131550763719 1101 11 -4.002188619639789 1101 12 -1.6519890204527812 1101 13 -0.9758150358152022 1101 14 -0.8677488066655528 1101 15 -2.01955160662742 1101 16 -0.37689313230550425 1101 17 -0.22366147488839516 1101 18 0.0398727202740119 1101 19 -0.8346077320712944 1101 20 -0.1932555208037101 1101 21 -0.4517326381913449 1101 22 -0.1398957209347813 1101 23 -0.2841273326691933 1101 24 -0.2172443364737262 1101 25 -0.04389983025213695 1102 1 -0.8206744352847863 1102 2 -0.5288112631064692 1102 3 -0.6486775788599601 1102 4 -0.6150349870153562 1102 5 -0.4575600446528867 1102 6 -1.2108094227776682 1102 7 -0.22450738052177854 1102 8 0.3058603011167236 1102 9 0.2821335130500202 1102 10 0.11251340738081694 1102 11 0.041850661818699564 1102 12 -0.2602843052408801 1102 13 -0.07542707691010975 1102 14 -0.20445303878686616 1102 15 0.2744585655262455 1102 16 -0.13334239169046214 1102 17 -0.08097261743480288 1102 18 -0.4351242449261221 1102 19 0.05391230132389069 1102 20 -0.1099434517143223 1102 21 -0.16380197531673701 1102 22 -0.21688178067450536 1102 23 -0.1619264710337731 1102 24 -3.8226014395157115 1102 25 -1.8744366058330186 1103 1 -0.3366120153545573 1103 2 -0.2666652946810615 1103 3 0.3255744462893375 1103 4 -0.0023484860397360308 1103 5 -0.22100191153504412 1103 6 0.4767681833086215 1103 7 0.30020091914119007 1103 8 0.503876541730083 1103 9 0.2818230887048685 1103 10 0.07589072570866762 1103 11 -0.0649500950254236 1103 12 -0.128486997556152 1103 13 -0.08946761931505876 1103 14 -0.06155190154974122 1103 15 0.21595096752084686 1103 16 0.4034798484892832 1103 17 -0.14033511423200906 1103 18 -0.11860474239345109 1103 19 -1.5132083404168526 1103 20 -0.5384670144003323 1103 21 -0.27342365180529526 1103 22 -0.2420335436876897 1103 23 0.0027290072168142387 1103 24 1.4133456996529397 1103 25 0.36772641129451955 1104 1 -1.2542179644912679 1104 2 -0.8934142619116976 1104 3 -2.12851065005432 1104 4 -1.2085713427423848 1104 5 -1.422228234876112 1104 6 -2.278278905196583 1104 7 -1.1350872933252276 1104 8 -0.5880206724407628 1104 9 -0.7445065115724674 1104 10 -0.7404508633598681 1104 11 -0.6876570581538672 1104 12 -0.609569744289324 1104 13 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0.8786282598870621 1110 9 0.5383133858970623 1110 10 0.47140836421030485 1110 11 0.1670803608806537 1110 12 0.10929473822534419 1110 13 0.06273148174147991 1110 14 -0.052132344866883784 1110 15 0.46510937366797833 1110 16 0.28124358675126615 1110 17 -0.23496779698143655 1110 18 -0.03350584928752909 1110 19 -1.6960929791143151 1110 20 -0.14131864210345668 1110 21 -0.3053540955886902 1110 22 -0.058047372869786784 1110 23 0.09330292293620346 1110 24 -0.9848587447086103 1110 25 -0.5063853691543905 1111 1 0.26950771715984345 1111 2 -0.0845134166486794 1111 3 0.6704550590348823 1111 4 0.5533451230169781 1111 5 0.6869454272376458 1111 6 0.7962631043531232 1111 7 0.8904068939553831 1111 8 1.2443455501071803 1111 9 0.4129809815289107 1111 10 0.41913135371833476 1111 11 -0.08129088476211166 1111 12 0.274794789073205 1111 13 0.21809766207179848 1111 14 0.3981957861172063 1111 15 -0.5119387673558577 1111 16 -0.4047600526573376 1111 17 0.13969114369534452 1111 18 0.31199346588915233 1111 19 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16 -0.6345564010008431 2213 17 -0.06867630989767548 2213 18 0.17632582670935193 2213 19 1.0369273291523768 2213 20 -0.9383552865197942 2213 21 0.14484873367362638 2213 22 -0.22184493638459202 2213 23 0.06166324737855433 2213 24 -0.16768804926081415 2213 25 0.23604047304634435 2214 1 1.23100467734022 2214 2 0.9629460188709089 2214 3 0.8719608769678047 2214 4 1.0424762580171816 2214 5 1.263277109808005 2214 6 0.7851969530466043 2214 7 1.369778396149691 2214 8 -2.561653564117563 2214 9 -0.8714998333843234 2214 10 -0.19844121945878612 2214 11 0.22210525808927883 2214 12 -0.9547840650557156 2214 13 -0.10851585807959196 2214 14 0.35833791278307103 2214 15 -0.8210783574834934 2214 16 0.0743457169605648 2214 17 0.4035092376680467 2214 18 0.45773570529004104 2214 19 1.3155787496404088 2214 20 -0.6731798375827012 2214 21 0.17770857099532833 2214 22 0.6926055331819896 2214 23 0.30793029127026017 2214 24 -1.809506981242425 2214 25 -0.926777319401296 2215 1 0.8149579506610962 2215 2 0.07901256098342202 2215 3 0.5616304310261917 2215 4 0.8083644612291678 2215 5 0.9673109658913432 2215 6 1.2823557973350432 2215 7 1.0795379688912021 2215 8 -0.29215989562032973 2215 9 0.18387109265692914 2215 10 0.059566102815651334 2215 11 0.03186483083079139 2215 12 -0.11600903627616077 2215 13 0.13604810802658562 2215 14 0.4503032263387112 2215 15 0.2769268888883971 2215 16 0.4977949233996384 2215 17 -0.09935267706897832 2215 18 -0.5225734154708661 2215 19 0.5034686890617976 2215 20 -3.472363630445372 2215 21 -1.6130829140298637 2215 22 -0.7571359225918499 2215 23 -0.6778639301719238 2215 24 -0.5112818779190924 2215 25 -0.24786188412227617 2216 1 0.35158822176277127 2216 2 -0.750333838526597 2216 3 0.09891462497418502 2216 4 -0.27266118451964516 2216 5 -0.10417293240983838 2216 6 -1.7811741887115353 2216 7 -9.615665829387414 2216 8 -10.30785864902234 2216 9 -5.709613164841308 2216 10 -3.2234857827386385 2216 11 -2.137023177974143 2216 12 -1.7865553381582586 2216 13 -1.4514748598621203 2216 14 -1.5532813760808004 2216 15 -2.5603034255011288 2216 16 -1.5687117931248054 2216 17 -1.9027530758698106 2216 18 -1.78031664483661 2216 19 -1.3035881515698335 2216 20 -1.913713658766678 2216 21 -4.357965507248405 2216 22 -3.270528907197029 2216 23 -2.984137444915124 2216 24 -3.189340924389061 2216 25 -2.569243779669616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46411.0 3 4.0 4 379.0 5 1905.0 6 2493.0 7 2142.0 8 1704.0 9 1441.0 10 1415.0 11 1392.0 12 1314.0 13 1276.0 14 1263.0 15 1323.0 16 1395.0 17 1514.0 18 1640.0 19 2121.0 20 2797.0 21 3521.0 22 4414.0 23 5062.0 24 5953.0 25 6691.0 26 7677.0 27 8273.0 28 9349.0 29 10987.0 30 13116.0 31 15684.0 32 20212.0 33 26145.0 34 35460.0 35 48613.0 36 70361.0 37 110739.0 38 133266.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41442477046568 18.220329924236818 12.67253103626338 25.692714269034123 2 15.334843837029904 20.075842839703135 39.555425572641376 25.03388775062559 3 18.64413463355323 24.452897151729747 29.653721975304588 27.24924623941244 4 12.197376717547224 16.722440427711952 37.32069201061398 33.759490844126844 5 13.874614931389267 37.25166459600825 35.061635595849054 13.812084876753428 6 32.47657185997276 36.2421427516371 17.945544960693244 13.335740427696905 7 27.917161788957756 31.549686139099464 22.510755537414788 18.02239653452799 8 25.641413858818186 35.190292968557145 20.74428693210805 18.42400624051662 9 26.275780238391818 15.802525056085917 19.7776913444701 38.144003361052164 10 14.911864590394808 29.067860736368516 33.80315905408932 22.217115619147354 11 34.067787987350265 23.446313312250446 23.280396362627823 19.20550233777146 12 22.992025706797435 25.63754137721082 30.43032299251805 20.9401099234737 13 28.266955939502154 22.26345053040647 25.435550362356896 24.03404316773448 14 21.420061049660287 21.820494305313947 27.218794105097317 29.54065053992845 15 23.884374015334522 29.49844422854742 23.179517645205337 23.43766411091272 16 22.704893011530924 28.378121112456878 26.3195900990944 22.5973957769178 17 22.219523443000416 28.30293650884826 26.935038378281682 22.542501669869644 18 22.199137438704977 27.85255715945568 27.75999921227279 22.188306189566553 19 23.49269653700968 27.54849827671098 27.277203348104383 21.681601838174956 20 23.50485280747303 27.18212223757586 27.33359810154627 21.979426853404842 21 23.513792497944543 26.933977513854856 27.030965630759184 22.521264357441417 22 23.19980569044716 27.119311645280035 26.930089589176433 22.750793075096375 23 22.6352792894514 27.15087998004372 27.00022319523675 23.21361753526813 24 23.279888405678182 27.154837121522934 27.022565028308854 22.54270944449003 25 22.76467460515433 26.897425461802044 27.122254460476046 23.21564547256758 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 444.0 1 444.0 2 568.0 3 692.0 4 692.0 5 692.0 6 876.5 7 1061.0 8 1061.0 9 1061.0 10 1350.5 11 1640.0 12 1640.0 13 1640.0 14 2494.0 15 3348.0 16 3348.0 17 3348.0 18 5416.5 19 7485.0 20 7485.0 21 7485.0 22 11072.5 23 14660.0 24 14660.0 25 14660.0 26 19995.0 27 25330.0 28 25330.0 29 25330.0 30 32010.5 31 38691.0 32 38691.0 33 38691.0 34 44972.0 35 51253.0 36 51253.0 37 51253.0 38 56856.0 39 62459.0 40 62459.0 41 62459.0 42 67326.5 43 72194.0 44 72194.0 45 72194.0 46 76641.5 47 81089.0 48 81089.0 49 81089.0 50 79471.5 51 77854.0 52 77854.0 53 77854.0 54 70493.0 55 63132.0 56 63132.0 57 63132.0 58 54927.0 59 46722.0 60 46722.0 61 46722.0 62 38549.0 63 30376.0 64 30376.0 65 30376.0 66 23717.0 67 17058.0 68 17058.0 69 17058.0 70 12699.5 71 8341.0 72 8341.0 73 8341.0 74 5919.5 75 3498.0 76 3498.0 77 3498.0 78 2435.0 79 1372.0 80 1372.0 81 1372.0 82 944.0 83 516.0 84 516.0 85 516.0 86 341.5 87 167.0 88 167.0 89 167.0 90 108.0 91 49.0 92 49.0 93 49.0 94 29.5 95 10.0 96 10.0 97 10.0 98 10.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22528435381293357 2 0.13536751048482898 3 0.1373364924555174 4 0.07285233291547161 5 0.023791865479151762 6 0.02329961998647966 7 0.30486404179492393 8 0.2969881139121703 9 0.019525737875993515 10 0.0359339209650637 11 0.018213083228867902 12 0.019525737875993515 13 0.017720837736195795 14 0.0183771650597586 15 0.017720837736195795 16 0.021986965339354042 17 0.020182065199556325 18 0.01706451041263299 19 0.024940438295386676 20 0.01870532872154001 21 0.01624410125817948 22 0.01886941055243071 23 0.01968981970688422 24 0.016736346750851585 25 0.01640818308907018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 609452.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.74808724112644 #Duplication Level Percentage of deduplicated Percentage of total 1 92.15518345694048 79.94285894244683 2 6.023575195063984 10.450672530497913 3 1.0205907548720627 2.656028875233863 4 0.32211673400349694 1.117720421726483 5 0.14599584547107966 0.6332430169883617 6 0.08509987392892097 0.4429350772676938 7 0.05435864026109431 0.33008556473748923 8 0.03839004154333312 0.26642101383932243 9 0.023494823046181754 0.18343178633925025 >10 0.11739729557451038 1.7079272315786664 >50 0.005859193709593386 0.360217318657942 >100 0.006993128253993468 1.1411399671114508 >500 5.670103987436733E-4 0.38857783354105224 >1k 3.780069324957822E-4 0.37874042003368386 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1252 0.20543045227515866 No Hit GTACATGGGAAGCAGTGGTATCAAC 1058 0.1735985770823625 No Hit CCCATGTACTCTGCGTTGATACCAC 943 0.15472916652993182 No Hit GGTATCAACGCAGAGTACTTTTTTT 735 0.12060014570466583 No Hit TATCAACGCAGAGTACTTTTTTTTT 692 0.11354462697636565 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGA 95 3.1286618E-10 14.970657 19 TAGGACC 120 7.0940587E-10 12.696054 4 TATGGCG 185 0.0 11.787707 16 CAGGACT 75 2.0257676E-4 11.426451 4 TAGGACA 75 2.0257676E-4 11.426451 4 TGGCGAG 185 0.0 11.275198 18 TTACACT 110 4.8024594E-7 11.253322 4 GTATAAA 85 5.0571536E-5 11.230164 1 ATTACAC 85 5.1084822E-5 11.219042 3 GTGTTAC 60 0.005662952 11.136578 1 GGATTGG 60 0.005662952 11.136578 1 CTTACTC 60 0.0057048905 11.125549 3 GTGTAGT 105 3.2614334E-6 10.909301 1 CCTGGAC 115 8.361385E-7 10.780036 3 CTACAGT 195 1.8189894E-12 10.742816 4 TGCGTTG 230 0.0 10.7181225 12 TAGGACT 80 3.6837978E-4 10.7122965 4 GTAGGAC 205 0.0 10.699136 3 AACAGGG 90 9.318412E-5 10.574818 7 TCCTACA 210 0.0 10.444394 2 >>END_MODULE