FastQCFastQC Report
Thu 26 May 2016
SRR1926799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609452
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24030.39428863963035643No Hit
GGTATCAACGCAGAGTACTTTTTTT15300.25104520126277374No Hit
TATCAACGCAGAGTACTTTTTTTTT13900.2280737449380755No Hit
GTACATGGGAAGCAGTGGTATCAAC11760.19296023312746532No Hit
CCCATGTACTCTGCGTTGATACCAC11250.18459205975203954No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7450.12224096401357286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG456.5480894E-414.8382191
ATTACAC602.5011492E-514.2870673
TGTGCGC400.00532161714.23080110
TACTATA551.8944193E-413.8712652
TTGTAGT801.9399577E-613.094324
GCATTAG603.9473362E-412.7184731
GTCCTAG701.0480757E-412.2642421
AAGGTTG550.003009399912.1203635
GTATAAA802.7426113E-511.9235681
ATAACAC657.838362E-411.7227213
TCCTGTA751.9974214E-411.4437932
TAGACAA1001.8675491E-611.4277725
TAGACTG855.180252E-511.2036985
GTTCTAG600.00569301911.1286641
CTAAGGT600.00575627411.1121643
TTAAACC600.00575627411.1121643
AGGGTTG600.005763339311.1103335
TAGGACC600.005763339311.1103334
TAAGACA600.005763339311.1103334
GGTTAAT700.001439271610.9015481
Produced by FastQC (version 0.11.5)