##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926799_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609452 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.701011728569274 31.0 30.0 33.0 25.0 34.0 2 29.974378622106418 31.0 30.0 34.0 26.0 34.0 3 30.056798566581126 31.0 30.0 34.0 26.0 34.0 4 33.707374821971214 35.0 35.0 37.0 28.0 37.0 5 33.69387745056214 37.0 35.0 37.0 28.0 37.0 6 33.638302934439466 37.0 35.0 37.0 29.0 37.0 7 33.59677874549595 37.0 35.0 37.0 28.0 37.0 8 33.62143204058728 37.0 35.0 37.0 28.0 37.0 9 35.11051567637812 39.0 35.0 39.0 29.0 39.0 10 35.01254733760821 39.0 35.0 39.0 28.0 39.0 11 35.056590182655896 39.0 35.0 39.0 28.0 39.0 12 34.945032586651614 39.0 35.0 39.0 27.0 39.0 13 34.943516470534185 39.0 35.0 39.0 27.0 39.0 14 36.02874221431713 40.0 36.0 41.0 27.0 41.0 15 35.994424499386334 40.0 36.0 41.0 27.0 41.0 16 35.970949311840805 40.0 36.0 41.0 27.0 41.0 17 35.947738296043 40.0 36.0 41.0 27.0 41.0 18 35.92597120035704 40.0 36.0 41.0 27.0 41.0 19 36.000036098002795 40.0 36.0 41.0 27.0 41.0 20 35.96364110709293 40.0 36.0 41.0 27.0 41.0 21 35.920351397649036 40.0 36.0 41.0 27.0 41.0 22 35.82675583967236 40.0 36.0 41.0 27.0 41.0 23 35.76551229629241 40.0 36.0 41.0 27.0 41.0 24 35.68600644513432 40.0 36.0 41.0 27.0 41.0 25 35.56122221274194 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08977812852452871 1101 2 -0.17882823301172834 1101 3 0.01748088895171307 1101 4 -0.02738961395943562 1101 5 -0.0025732745571289684 1101 6 -0.04135570156189061 1101 7 -0.06421547796109195 1101 8 0.20494089572634522 1101 9 0.3058585147666619 1101 10 0.2609826877190855 1101 11 0.22714325643592304 1101 12 0.33098568128200867 1101 13 0.1614283342368097 1101 14 0.2142095095507841 1101 15 0.04139383579612854 1101 16 0.1206744074891617 1101 17 0.1521767111294281 1101 18 0.2636111968700732 1101 19 0.1378604682558091 1101 20 -0.05300751140456583 1101 21 0.26960273656229816 1101 22 0.06992965817185137 1101 23 0.2281216076581316 1101 24 0.04811516803609095 1101 25 0.13253606908837412 1102 1 -0.3897179280047709 1102 2 -0.2814577322573655 1102 3 -0.10932048153912888 1102 4 -0.0867069450201825 1102 5 -0.11421303266499905 1102 6 -0.12195503118079642 1102 7 -0.13599035279107596 1102 8 -0.1315094041614273 1102 9 -0.08451212719879919 1102 10 -0.11171427079210616 1102 11 -0.2880728584752248 1102 12 -0.17869594963183033 1102 13 -0.13016266888222106 1102 14 -0.16633209693600293 1102 15 -0.2614246881267661 1102 16 -0.11082879701175585 1102 17 -0.27192356361285874 1102 18 -0.3252304014413383 1102 19 -0.2227555094080671 1102 20 -0.1544181359292196 1102 21 -0.12770335211331485 1102 22 -0.1395186028258948 1102 23 0.05665046055332823 1102 24 -0.056829449963117895 1102 25 -0.15066402207948926 1103 1 -0.5712718235839347 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2214 6 0.28880308052713843 2214 7 0.2602103546633927 2214 8 0.33240823416338827 2214 9 0.30771460759311253 2214 10 0.10559652880613157 2214 11 0.15788111331393395 2214 12 0.21430592817942795 2214 13 0.42779619395021484 2214 14 0.28959174253296993 2214 15 0.18419302443709995 2214 16 0.043885382646742244 2214 17 0.11383127284288008 2214 18 0.20709197071465013 2214 19 0.1886554692372897 2214 20 0.23824311565382317 2214 21 0.41615664177287925 2214 22 0.4338797988510379 2214 23 0.3356822960046557 2214 24 0.38151938623060033 2214 25 0.47464931038044256 2215 1 0.28696064977796 2215 2 0.1874669018884525 2215 3 0.18347540108743132 2215 4 0.20300005009890754 2215 5 0.14887079601214026 2215 6 0.2049847408717369 2215 7 -0.04829895859030131 2215 8 -0.029388553849216237 2215 9 -0.013297300538759771 2215 10 -0.13318321793376242 2215 11 -0.11841019242876882 2215 12 -0.08078078793145949 2215 13 -0.26992786198592 2215 14 -0.14797454480968497 2215 15 -0.15365445334130357 2215 16 -0.15884159842480017 2215 17 -0.10876020840794354 2215 18 -0.38204256664216274 2215 19 -0.24650843270462275 2215 20 -0.28270557180961475 2215 21 -0.36627650472922824 2215 22 -0.19875621994531656 2215 23 -0.14689993804391577 2215 24 -0.27754664634219495 2215 25 -0.2313566531214164 2216 1 -0.2615415445476472 2216 2 0.017868788133508673 2216 3 -1.8096252523749072 2216 4 -1.7144933070580919 2216 5 -0.8017451348378231 2216 6 -0.45645913919328507 2216 7 -0.4304735744106267 2216 8 -0.37943621653555226 2216 9 -0.37001313422130977 2216 10 -0.421741870668491 2216 11 -0.6140487112474702 2216 12 -0.3174934901524935 2216 13 -0.3152097165479546 2216 14 -0.5091886308604074 2216 15 -0.5309209637419059 2216 16 -0.5071512999688892 2216 17 -0.304341163595808 2216 18 -0.5317531367063069 2216 19 -0.5294700584857637 2216 20 -0.4015776930524382 2216 21 -0.27297460881348456 2216 22 -0.26562765963475243 2216 23 -0.5132763305081482 2216 24 -0.5173594954656195 2216 25 -0.44086340188150075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16197.0 3 14.0 4 222.0 5 1870.0 6 2505.0 7 1902.0 8 1529.0 9 1269.0 10 1032.0 11 961.0 12 845.0 13 817.0 14 787.0 15 781.0 16 808.0 17 848.0 18 978.0 19 1169.0 20 1513.0 21 1943.0 22 2461.0 23 2957.0 24 3566.0 25 4169.0 26 4843.0 27 5510.0 28 6404.0 29 7684.0 30 9657.0 31 12359.0 32 16144.0 33 21253.0 34 29221.0 35 40440.0 36 59307.0 37 103270.0 38 242217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.103430148508345 19.193060848994612 10.674694440793797 28.028814561703246 2 15.057592861507729 21.14903179599051 37.79461721937854 25.998758123123224 3 18.284625534177515 24.72249026828676 29.258157899919258 27.73472629761647 4 12.6438177247757 17.34082421585293 36.660639394078615 33.35471866529275 5 13.634431822097659 38.1138899235822 34.41229511021262 13.839383144107526 6 32.14705601459561 37.2594705140407 17.287825247898095 13.3056482234656 7 28.440811155022782 32.20297257198377 21.181380722923205 18.174835550070245 8 26.280058944577 35.80554853573925 19.65027092844273 18.264121591241018 9 26.39828811857142 15.871005008303413 19.535993488549167 38.194713384576 10 15.180210854493989 29.314025515855107 33.115477704393484 22.390285925257412 11 34.261049066486386 23.42234722308826 23.06167523768579 19.254928472739564 12 23.047489031021616 25.564529096917525 30.412538600630732 20.97544327143012 13 28.47968233392677 22.077628005808563 25.300888513319496 24.14180114694517 14 21.65387922264592 21.854879465487027 27.055945341060493 29.435295970806564 15 23.722870274501055 29.233074021262382 23.49790707099878 23.546148633237777 16 22.962695567278427 28.32084929731149 26.057970776690265 22.658484358719818 17 22.326523915005332 28.21183033883009 26.869472475182544 22.592173270982034 18 22.252304138013642 27.636201343184887 28.018843312051743 22.092651206749725 19 23.142543510613685 27.348637867975828 27.704204945778894 21.804613675631597 20 23.362441832227407 26.86169689093666 27.843739539645973 21.932121737189963 21 23.258321312702158 26.57224112155361 27.553912325700846 22.61552524004339 22 23.236978854739757 26.7220826612804 27.16838597360575 22.872552510374092 23 22.744240308080553 26.945234317443074 27.219579588843075 23.0909457856333 24 22.638899599803757 26.9772303269664 27.753666824732427 22.63020324849742 25 22.864916132547002 26.58176543367891 27.377447843338025 23.17587059043606 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 345.0 1 345.0 2 324.5 3 304.0 4 304.0 5 304.0 6 575.5 7 847.0 8 847.0 9 847.0 10 1195.0 11 1543.0 12 1543.0 13 1543.0 14 2257.0 15 2971.0 16 2971.0 17 2971.0 18 5061.5 19 7152.0 20 7152.0 21 7152.0 22 10681.5 23 14211.0 24 14211.0 25 14211.0 26 19671.0 27 25131.0 28 25131.0 29 25131.0 30 31747.5 31 38364.0 32 38364.0 33 38364.0 34 44724.0 35 51084.0 36 51084.0 37 51084.0 38 56466.5 39 61849.0 40 61849.0 41 61849.0 42 66968.0 43 72087.0 44 72087.0 45 72087.0 46 76842.0 47 81597.0 48 81597.0 49 81597.0 50 80413.0 51 79229.0 52 79229.0 53 79229.0 54 71758.0 55 64287.0 56 64287.0 57 64287.0 58 55841.0 59 47395.0 60 47395.0 61 47395.0 62 39001.5 63 30608.0 64 30608.0 65 30608.0 66 23931.5 67 17255.0 68 17255.0 69 17255.0 70 12610.0 71 7965.0 72 7965.0 73 7965.0 74 5660.5 75 3356.0 76 3356.0 77 3356.0 78 2322.5 79 1289.0 80 1289.0 81 1289.0 82 851.5 83 414.0 84 414.0 85 414.0 86 269.0 87 124.0 88 124.0 89 124.0 90 81.5 91 39.0 92 39.0 93 39.0 94 22.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12010790021199373 2 0.11420095429992846 3 0.01706451041263299 4 0.0 5 0.006399191404737371 6 0.3521196090914461 7 0.02428411097182387 8 0.01082940083878632 9 0.010501237177004916 10 0.004430209434048949 11 0.005578782250283861 12 9.84490985344211E-4 13 0.0011485728162349126 14 0.0 15 0.0031175547869233345 16 0.0011485728162349126 17 3.281636617814036E-4 18 8.20409154453509E-4 19 4.922454926721055E-4 20 0.0013126546471256145 21 0.01361879196392825 22 1.640818308907018E-4 23 1.640818308907018E-4 24 1.640818308907018E-4 25 0.006235109573846669 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 609452.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.74912753935548 #Duplication Level Percentage of deduplicated Percentage of total 1 89.6038739872093 74.14642396587918 2 7.807685576356553 12.921583390902292 3 1.4749429328725285 3.6615072259661963 4 0.4653630828645932 1.540335563842795 5 0.20539322647204986 0.849805514652769 6 0.12635910299810735 0.6273663317849365 7 0.07075841643323952 0.4098638058141874 8 0.04163323153148547 0.2756090868699526 9 0.03641012839681072 0.27116157225888 >10 0.15052721787163936 2.0668352571487736 >50 0.007138537576796188 0.4164482745053457 >100 0.00872481228700648 1.4398391035893814 >500 1.982911883137461E-4 0.12224275009619875 >1k 9.914559415687303E-4 1.2509781566891534 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2403 0.39428863963035643 No Hit GGTATCAACGCAGAGTACTTTTTTT 1530 0.25104520126277374 No Hit TATCAACGCAGAGTACTTTTTTTTT 1390 0.2280737449380755 No Hit GTACATGGGAAGCAGTGGTATCAAC 1176 0.19296023312746532 No Hit CCCATGTACTCTGCGTTGATACCAC 1125 0.18459205975203954 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 745 0.12224096401357286 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.640818308907018E-4 0.0 10 0.0 0.0 0.0 1.640818308907018E-4 0.0 11 0.0 0.0 0.0 1.640818308907018E-4 0.0 12 0.0 0.0 0.0 1.640818308907018E-4 0.0 13 0.0 0.0 0.0 1.640818308907018E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 45 6.5480894E-4 14.838219 1 ATTACAC 60 2.5011492E-5 14.287067 3 TGTGCGC 40 0.005321617 14.230801 10 TACTATA 55 1.8944193E-4 13.871265 2 TTGTAGT 80 1.9399577E-6 13.09432 4 GCATTAG 60 3.9473362E-4 12.718473 1 GTCCTAG 70 1.0480757E-4 12.264242 1 AAGGTTG 55 0.0030093999 12.120363 5 GTATAAA 80 2.7426113E-5 11.923568 1 ATAACAC 65 7.838362E-4 11.722721 3 TCCTGTA 75 1.9974214E-4 11.443793 2 TAGACAA 100 1.8675491E-6 11.427772 5 TAGACTG 85 5.180252E-5 11.203698 5 GTTCTAG 60 0.005693019 11.128664 1 CTAAGGT 60 0.005756274 11.112164 3 TTAAACC 60 0.005756274 11.112164 3 AGGGTTG 60 0.0057633393 11.110333 5 TAGGACC 60 0.0057633393 11.110333 4 TAAGACA 60 0.0057633393 11.110333 4 GGTTAAT 70 0.0014392716 10.901548 1 >>END_MODULE