FastQCFastQC Report
Thu 26 May 2016
SRR1926798_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926798_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600815
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11540.19207243494253637No Hit
GTCCTACAGTGGACATTTCTAAATT7950.13232026497341112No Hit
GTCCTAAAGTGTGTATTTCTCATTT6660.11084942952489535No Hit
GGTATCAACGCAGAGTACTTTTTTT6490.10801993958206771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2200.013.4137934
GTCTTAC500.001450766913.3562631
GTATAGA1102.564775E-913.0093471
ATTATAC603.987332E-412.7032343
GAACAGT1457.2759576E-1212.4716986
GAACTGT1155.149559E-912.4146196
TTATAGC550.002999192612.12581353
TCTACTC550.002999192612.12581353
CCTATAC550.002999192612.12581353
GGCGAGG1650.012.07030619
AGAACTG1209.686119E-911.8973455
GTCCTAC4100.011.6343761
ACTGAAC1001.898863E-611.4119188
GTGTAAG1104.6852074E-711.2747671
GGTATCA2650.011.160221
GTATAGG1201.1972588E-711.1302191
GACTGTC951.3282517E-511.0206987
GTCCTAA2450.010.9030711
GTACTAG700.001437541310.9030711
GTAGAAC1951.8189894E-1210.7488893
Produced by FastQC (version 0.11.5)