##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926798_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600815 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.480395795710827 31.0 28.0 31.0 22.0 34.0 2 28.543897872057123 31.0 29.0 31.0 22.0 34.0 3 28.086435924535838 31.0 28.0 31.0 16.0 34.0 4 31.911228914058405 35.0 32.0 37.0 19.0 37.0 5 31.75536229954312 35.0 32.0 37.0 19.0 37.0 6 31.378903655867447 35.0 32.0 37.0 17.0 37.0 7 31.207364995880596 35.0 32.0 37.0 17.0 37.0 8 31.347016968617627 35.0 32.0 37.0 17.0 37.0 9 33.46756322661718 37.0 33.0 39.0 18.0 39.0 10 33.647570383562325 38.0 34.0 39.0 19.0 39.0 11 33.77875718815276 38.0 34.0 39.0 23.0 39.0 12 33.681324534174415 38.0 34.0 39.0 21.0 39.0 13 33.784059985186786 38.0 34.0 39.0 23.0 39.0 14 34.83026555595316 39.0 35.0 41.0 23.0 41.0 15 34.41502126278472 39.0 34.0 41.0 17.0 41.0 16 34.502224478416814 39.0 34.0 41.0 18.0 41.0 17 34.738165658314124 39.0 34.0 41.0 22.0 41.0 18 34.78650999059611 39.0 35.0 41.0 22.0 41.0 19 34.196037049674196 39.0 34.0 41.0 16.0 41.0 20 34.45567437563976 39.0 34.0 41.0 18.0 41.0 21 34.451225418806125 39.0 34.0 41.0 18.0 41.0 22 34.46764145369207 39.0 34.0 41.0 18.0 41.0 23 34.469860106688415 39.0 35.0 41.0 17.0 41.0 24 33.19269325832411 39.0 33.0 41.0 2.0 41.0 25 33.778565781480154 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2371653852685185 1101 2 -0.4890268357585761 1101 3 -1.104663169137993 1101 4 -0.37697286291849963 1101 5 -0.5082307218701168 1101 6 -2.0747221245185052 1101 7 -0.6631677731156564 1101 8 -0.4006034259851141 1101 9 -0.39829364934970357 1101 10 -9.69312096403453 1101 11 -4.288862223209144 1101 12 -1.7225804965647065 1101 13 -0.9566533036659806 1101 14 -0.7873852732007549 1101 15 -2.003804838318544 1101 16 -0.5871781484988148 1101 17 -0.2591464360382645 1101 18 -0.11177613384224827 1101 19 -0.8514296336886957 1101 20 -0.3660969731795092 1101 21 -0.5104530869069492 1101 22 0.10984222814438027 1101 23 -0.36472145704301084 1101 24 -0.42429017794089674 1101 25 -0.2758513972273633 1102 1 0.12170858378608074 1102 2 -0.20993747579674604 1102 3 -0.3537668430525187 1102 4 -0.1061596940820202 1102 5 -0.0905086851991328 1102 6 -1.1830133112133474 1102 7 -0.06288967346328178 1102 8 0.7552714291713158 1102 9 0.5429537093852161 1102 10 0.5660883604832279 1102 11 0.3497963549382206 1102 12 0.20468008553604022 1102 13 0.2506917989173445 1102 14 0.3515268663943658 1102 15 0.602078877856222 1102 16 0.5533139788271981 1102 17 0.20521727468356943 1102 18 0.18192572697160614 1102 19 0.6938229053338674 1102 20 0.05639092812237578 1102 21 0.43878827360017425 1102 22 0.3583310837097855 1102 23 0.05067217665612134 1102 24 -3.8054748279306736 1102 25 -1.4266754719325618 1103 1 -0.25450703320276347 1103 2 -0.5016966388259156 1103 3 -0.13269014382159128 1103 4 -0.515173759071974 1103 5 -0.8413020060610492 1103 6 0.040919343120471297 1103 7 -0.21356105155515337 1103 8 0.022691923870961972 1103 9 -0.17442452105890283 1103 10 -0.12987089954587105 1103 11 -0.43101705876647856 1103 12 -0.36202953824280115 1103 13 -0.3335304694953152 1103 14 -0.5104935035910856 1103 15 0.022562491796499273 1103 16 -0.2110925894538198 1103 17 -0.6735771547489833 1103 18 -0.9088177082116573 1103 19 -2.2192374424801713 1103 20 -1.0019032588103443 1103 21 -0.8699735915699236 1103 22 -0.7443738286438517 1103 23 -1.0123445211890925 1103 24 0.4233951228764994 1103 25 -0.37567983692944296 1104 1 -0.2531534966393103 1104 2 -0.1436961996252606 1104 3 -1.5416698008458063 1104 4 -0.5345744498148193 1104 5 -0.626301307332735 1104 6 -2.0600289310205895 1104 7 -0.6615337412598912 1104 8 0.16724883030126136 1104 9 0.07390751463613299 1104 10 0.1710933455280923 1104 11 -0.17690354536087938 1104 12 -0.10178717933601433 1104 13 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25 -1.8903197579297917 1106 1 -0.3888272474729284 1106 2 -0.962978219934044 1106 3 -0.42204930080176695 1106 4 -1.0291650654956186 1106 5 -0.48435121107362633 1106 6 0.0021720680608581233 1106 7 -2.796564506425419 1106 8 -0.557557078623784 1106 9 -1.0566034765180987 1106 10 -0.26494530910933634 1106 11 -0.3921766569836933 1106 12 -0.22343729950261348 1106 13 -0.42834396683661424 1106 14 -1.1985242275398704 1106 15 -0.3784870857229734 1106 16 -0.39535364858011235 1106 17 -0.7517642528940556 1106 18 -0.959525864800959 1106 19 -0.37852086513645844 1106 20 -0.20240115886831944 1106 21 -0.7386465488811567 1106 22 -1.046534630950248 1106 23 -0.9815589251387209 1106 24 -4.195127651198067 1106 25 -2.45693618200724 1107 1 0.3753397685777351 1107 2 0.2770522188841724 1107 3 0.8683287851878951 1107 4 0.6495018305931382 1107 5 0.8150664284154061 1107 6 0.6122001561104717 1107 7 -1.4377754188386866 1107 8 -1.3619864598384765 1107 9 0.07802891901204845 1107 10 0.699917970673333 1107 11 0.34525945421493276 1107 12 0.6012159621282436 1107 13 0.2880418597251335 1107 14 0.5365271864353289 1107 15 -0.7269206740864291 1107 16 0.10467543309334815 1107 17 0.1591416169993849 1107 18 -0.03696309079707305 1107 19 0.6372268610401974 1107 20 0.8702448614258387 1107 21 0.6516052741762479 1107 22 0.4503403008895859 1107 23 0.14270827727700208 1107 24 -3.6742785148025945 1107 25 -1.7317628431160763 1108 1 0.4797268955884135 1108 2 -0.11728226445848122 1108 3 0.6472114587159474 1108 4 0.6275917169301479 1108 5 0.6091401295506458 1108 6 -2.7975724483010183 1108 7 -1.1567489485165865 1108 8 0.3272117276787796 1108 9 0.10240184684915477 1108 10 0.5730267776411324 1108 11 0.4384229697632591 1108 12 0.5309161321935036 1108 13 0.4217089819099442 1108 14 0.3582069115467519 1108 15 -0.7797673531735043 1108 16 0.4118891700977372 1108 17 0.1033936198484966 1108 18 0.1996480397773226 1108 19 0.380992101370488 1108 20 -1.0584541740976192 1108 21 -0.1345417151117303 1108 22 0.13582512610285846 1108 23 0.16698723999488863 1108 24 -0.6256579459540035 1108 25 0.05841420572954803 1109 1 0.5044287829768663 1109 2 0.33909458147060434 1109 3 -0.8246040668309185 1109 4 0.4705910061980916 1109 5 0.725613319158164 1109 6 0.6706499318616608 1109 7 0.7174277868541807 1109 8 1.2665941804940672 1109 9 0.7018332612059481 1109 10 0.6577223474113296 1109 11 0.3693792992270488 1109 12 0.04062157324223392 1109 13 0.27973630560891394 1109 14 0.32745590467634145 1109 15 -1.39909136801991 1109 16 0.18634230126728824 1109 17 -0.011539650308648675 1109 18 0.255906430364071 1109 19 -1.138047620654305 1109 20 -0.06242637657727812 1109 21 0.22417264957278604 1109 22 0.5886641058075099 1109 23 0.42380631920202916 1109 24 -0.48431703147875993 1109 25 -0.3473664149764488 1110 1 0.18552970239746003 1110 2 0.4679771818695997 1110 3 0.9408450180853762 1110 4 0.7133798430443079 1110 5 0.7572010544344252 1110 6 0.5168107003584943 1110 7 0.780266064241637 1110 8 1.2224435603188581 1110 9 0.67186643841125 1110 10 0.6885738956809107 1110 11 0.11796655744545603 1110 12 0.18541296885329928 1110 13 0.14781571405717386 1110 14 -0.02166262251595441 1110 15 0.42911359338177846 1110 16 0.24658061334986314 1110 17 -0.2310990223270437 1110 18 0.04072514837951502 1110 19 -1.9406877021878302 1110 20 -0.30020502173297814 1110 21 -0.16521606584051796 1110 22 0.09680986866639074 1110 23 0.03545520462140672 1110 24 -0.4439319156838124 1110 25 -0.018076146956673256 1111 1 0.4190365584542093 1111 2 0.24307433762427522 1111 3 0.7523225492261325 1111 4 0.8972990807322532 1111 5 0.8454430868140221 1111 6 0.8172107784225098 1111 7 0.7732593948597 1111 8 1.1138221538089965 1111 9 0.2337173191769608 1111 10 0.22742559102761817 1111 11 0.035746909072919664 1111 12 0.12168897604876605 1111 13 -0.10485076593467824 1111 14 -0.002288746807437292 1111 15 -0.8129399992598465 1111 16 -0.5079526147667579 1111 17 -0.29665582238134647 1111 18 0.05643553089317521 1111 19 0.6449981580436486 1111 20 -0.10608867241349174 1111 21 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2214 24 -2.218182523725755 2214 25 -1.0475957938185445 2215 1 0.42548102233896756 2215 2 0.01941353221209141 2215 3 0.42066011082762245 2215 4 0.7092227969425622 2215 5 0.8550864542651055 2215 6 1.3695246617223056 2215 7 0.9284718288841347 2215 8 -0.291301340781736 2215 9 -0.00292864355596123 2215 10 -0.30440597809727876 2215 11 -0.03445109051883577 2215 12 -0.3151873543976862 2215 13 -0.06572907929309224 2215 14 -0.1894231474139758 2215 15 -0.2035328316163003 2215 16 -0.04015743790850479 2215 17 -0.4053543906022625 2215 18 -0.9318273799049521 2215 19 0.28581406799611386 2215 20 -3.3429241782018444 2215 21 -1.8946886664716303 2215 22 -0.8555269665525742 2215 23 -0.7608274090936717 2215 24 -1.0118377111121148 2215 25 -0.7867583219739913 2216 1 -0.9235460959895114 2216 2 -1.5432352594994612 2216 3 -0.5993481350594081 2216 4 -0.9674772522667681 2216 5 -0.695307885332447 2216 6 -2.2319595979433338 2216 7 -10.049405254199822 2216 8 -11.355550100573726 2216 9 -6.187631193131029 2216 10 -4.053640573583319 2216 11 -2.9020327846496237 2216 12 -2.3132402610728633 2216 13 -2.0987099674549228 2216 14 -2.3644726892352566 2216 15 -3.4045622375134386 2216 16 -2.4258494939273376 2216 17 -2.6303234685259014 2216 18 -2.2452641858207087 2216 19 -1.6835697514455603 2216 20 -2.645423213965053 2216 21 -5.582916997743489 2216 22 -4.0429320391030785 2216 23 -3.975063534058762 2216 24 -4.264316132157749 2216 25 -3.5652179370772608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31361.0 3 5.0 4 263.0 5 1372.0 6 1895.0 7 1509.0 8 1181.0 9 1083.0 10 1054.0 11 1011.0 12 993.0 13 961.0 14 1032.0 15 1023.0 16 1122.0 17 1213.0 18 1432.0 19 1719.0 20 2317.0 21 2973.0 22 3777.0 23 4501.0 24 5096.0 25 5925.0 26 6890.0 27 7592.0 28 8505.0 29 10093.0 30 11991.0 31 14734.0 32 19291.0 33 25828.0 34 35232.0 35 48841.0 36 71121.0 37 114189.0 38 151690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.47977912051808 20.13782452722885 13.630685636996478 24.751710715256596 2 13.361928186259805 21.540122777899978 43.15273250814853 21.945216527691684 3 18.47786419059776 26.373833301949208 30.244912854883676 24.90338965256936 4 11.030291118536113 18.83109528393968 40.83937678846852 29.29923680905569 5 11.875120697347654 38.8478980348807 36.59177326878384 12.685207998987808 6 28.23952067896045 40.077878015525656 19.845409309069844 11.837191996444048 7 25.673498351212586 32.4840338940602 23.882122135492757 17.96034561923446 8 22.93490982004077 38.06757945736758 22.885825476777597 16.111685245814055 9 25.450595550228485 16.555547232002397 21.497573684254334 36.49628353351479 10 13.267134189793078 30.674545297130262 36.22355197698347 19.834768536093183 11 32.3707581798357 24.079804899161832 25.706200111533757 17.843236809468717 12 21.785481228941656 26.2588395104476 33.3317796215158 18.62389963909494 13 27.38379022646007 23.6514605709111 26.783514559098688 22.18123464353014 14 19.52972316092624 23.76537763646352 29.477118742820995 27.227780459789248 15 21.97252310965287 32.18971139776569 24.46452025309502 21.37324523948642 16 20.367173466822706 30.2167315635004 29.279320522659514 20.13677444701738 17 20.21927604943533 30.068386426592795 28.92356301939058 20.78877450458129 18 19.69386990719547 29.710932623080446 30.7738149735736 19.821382496150484 19 22.12446101852931 28.50150665090649 28.776199910100388 20.597832420463817 20 21.742177563808042 29.020755508499967 29.686969376136158 19.55009755155583 21 21.894486967349287 28.299113400208416 28.48222638758028 21.324173244862017 22 21.21312506970883 29.551216637562362 29.02334392630091 20.212314366427893 23 20.9883654148681 29.160042549720416 29.008221951061163 20.843370084350326 24 21.631803063646746 28.562463169319585 29.526802747360676 20.278931019672992 25 21.241474827748654 28.775920935009765 29.297294417874518 20.68530981936706 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 213.0 2 277.5 3 342.0 4 342.0 5 342.0 6 561.0 7 780.0 8 780.0 9 780.0 10 1247.5 11 1715.0 12 1715.0 13 1715.0 14 2983.5 15 4252.0 16 4252.0 17 4252.0 18 7303.0 19 10354.0 20 10354.0 21 10354.0 22 15821.5 23 21289.0 24 21289.0 25 21289.0 26 29405.5 27 37522.0 28 37522.0 29 37522.0 30 47287.5 31 57053.0 32 57053.0 33 57053.0 34 63352.0 35 69651.0 36 69651.0 37 69651.0 38 73544.5 39 77438.0 40 77438.0 41 77438.0 42 78521.5 43 79605.0 44 79605.0 45 79605.0 46 78282.0 47 76959.0 48 76959.0 49 76959.0 50 71190.0 51 65421.0 52 65421.0 53 65421.0 54 55111.0 55 44801.0 56 44801.0 57 44801.0 58 36334.0 59 27867.0 60 27867.0 61 27867.0 62 21295.5 63 14724.0 64 14724.0 65 14724.0 66 10755.5 67 6787.0 68 6787.0 69 6787.0 70 4751.5 71 2716.0 72 2716.0 73 2716.0 74 1819.5 75 923.0 76 923.0 77 923.0 78 605.5 79 288.0 80 288.0 81 288.0 82 185.0 83 82.0 84 82.0 85 82.0 86 53.0 87 24.0 88 24.0 89 24.0 90 13.5 91 3.0 92 3.0 93 3.0 94 3.5 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20172598886512488 2 0.11767349350465617 3 0.12050298344748385 4 0.07340029792864691 5 0.02313524129723792 6 0.021970157203132414 7 0.3145727054084868 8 0.30724931967410934 9 0.018308464335943676 10 0.0337874387290597 11 0.016644058487221523 12 0.01714338024183817 13 0.015146093223371586 14 0.0174762614115826 15 0.015312533808243801 16 0.019639989014921398 17 0.018474904920815893 18 0.014979652638499373 19 0.02413388480647121 20 0.01780914258132703 21 0.015478974393116018 22 0.016976939656965954 23 0.017642701996454815 24 0.015478974393116018 25 0.015645414977988232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 600815.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.09531850801203 #Duplication Level Percentage of deduplicated Percentage of total 1 95.85212088866861 86.35827361133069 2 3.2405999781310806 5.83925774373553 3 0.42349251648210695 1.1446407947464485 4 0.14553463304871886 0.5244795647388392 5 0.07739959902568452 0.34866707633057326 6 0.051066228407433883 0.27604968680223885 7 0.03334710855714973 0.21030928567443613 8 0.022985867597219056 0.16567352498835552 9 0.018139561213058358 0.14708605905774666 >10 0.11441672202452464 2.0082228611018063 >50 0.010171121317461089 0.6109677690580653 >100 0.009246358213196183 1.466007704468305 >500 0.001294490149529422 0.7080955498445485 >1k 1.8492716421848886E-4 0.19226876812249621 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1154 0.19207243494253637 No Hit GTCCTACAGTGGACATTTCTAAATT 795 0.13232026497341112 No Hit GTCCTAAAGTGTGTATTTCTCATTT 666 0.11084942952489535 No Hit GGTATCAACGCAGAGTACTTTTTTT 649 0.10801993958206771 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.6644058487221523E-4 0.0 0.0 0.0 5 0.0 1.6644058487221523E-4 0.0 0.0 0.0 6 0.0 1.6644058487221523E-4 0.0 0.0 0.0 7 0.0 1.6644058487221523E-4 0.0 0.0 0.0 8 0.0 1.6644058487221523E-4 0.0 0.0 0.0 9 0.0 1.6644058487221523E-4 0.0 0.0 0.0 10 0.0 1.6644058487221523E-4 0.0 0.0 0.0 11 0.0 1.6644058487221523E-4 0.0 0.0 0.0 12 0.0 1.6644058487221523E-4 0.0 0.0 0.0 13 0.0 1.6644058487221523E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 220 0.0 13.413793 4 GTCTTAC 50 0.0014507669 13.356263 1 GTATAGA 110 2.564775E-9 13.009347 1 ATTATAC 60 3.987332E-4 12.703234 3 GAACAGT 145 7.2759576E-12 12.471698 6 GAACTGT 115 5.149559E-9 12.414619 6 TTATAGC 55 0.0029991926 12.1258135 3 TCTACTC 55 0.0029991926 12.1258135 3 CCTATAC 55 0.0029991926 12.1258135 3 GGCGAGG 165 0.0 12.070306 19 AGAACTG 120 9.686119E-9 11.897345 5 GTCCTAC 410 0.0 11.634376 1 ACTGAAC 100 1.898863E-6 11.411918 8 GTGTAAG 110 4.6852074E-7 11.274767 1 GGTATCA 265 0.0 11.16022 1 GTATAGG 120 1.1972588E-7 11.130219 1 GACTGTC 95 1.3282517E-5 11.020698 7 GTCCTAA 245 0.0 10.903071 1 GTACTAG 70 0.0014375413 10.903071 1 GTAGAAC 195 1.8189894E-12 10.748889 3 >>END_MODULE