FastQCFastQC Report
Thu 26 May 2016
SRR1926798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600815
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17770.2957649193179265No Hit
GGTATCAACGCAGAGTACTTTTTTT11180.18608057388713664No Hit
TATCAACGCAGAGTACTTTTTTTTT9660.1607816049865599No Hit
GTCCTACAGTGGACATTTCTAAATT7720.12849213152135017No Hit
CTGTAGGACGTGGAATATGGCAAGA6990.11634196882567846No Hit
CTTTAGGACGTGAAATATGGCGAGG6920.11517688473157293No Hit
GTCCTAAAGTGTGTATTTCTCATTT6110.1016951973569235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG350.002124220816.335641
GTCTTAG903.481182E-813.7642911
TAGGACC2550.013.43716054
TAGGACT1153.6379788E-1013.2424194
TATACTT1051.9324943E-812.6906515
GTAGGAC5200.012.26458553
GGCGAGG2250.012.23189419
AGGACGT4550.012.1328215
TTAGGAC2850.012.02372553
AGGACCT4150.011.9261545
GGTATCA4000.011.9114061
TGTAGCC1052.6062298E-711.7969772
GGACGTG4600.011.7930456
GGACCTG3850.011.6183936
GACGTGA2650.011.4618477
TAGGACG4900.011.4604354
TGGCGAG4900.011.4270518
AATACTG1001.8796145E-611.4215865
GTCTAGA600.00573629711.11731151
TAGCCAG600.005786461311.104325
Produced by FastQC (version 0.11.5)