##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926798_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600815 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.69850619575077 31.0 30.0 33.0 25.0 34.0 2 29.977092782304037 31.0 30.0 33.0 26.0 34.0 3 29.991210272712898 31.0 30.0 34.0 26.0 34.0 4 33.60327887952198 35.0 35.0 37.0 28.0 37.0 5 33.560164110416686 35.0 35.0 37.0 28.0 37.0 6 33.53861171908158 37.0 35.0 37.0 28.0 37.0 7 33.52016178024849 37.0 35.0 37.0 28.0 37.0 8 33.56763396386575 37.0 35.0 37.0 28.0 37.0 9 35.069730283032214 39.0 35.0 39.0 28.0 39.0 10 34.93550260895617 39.0 35.0 39.0 28.0 39.0 11 35.01129965130698 39.0 35.0 39.0 28.0 39.0 12 34.90028378119721 39.0 35.0 39.0 27.0 39.0 13 34.913772126195255 39.0 35.0 39.0 27.0 39.0 14 35.96589632415969 40.0 36.0 41.0 27.0 41.0 15 35.95288067042267 40.0 36.0 41.0 27.0 41.0 16 35.92698085101071 40.0 36.0 41.0 27.0 41.0 17 35.903164867721344 40.0 36.0 41.0 27.0 41.0 18 35.87584863893212 40.0 36.0 41.0 27.0 41.0 19 35.977207626307596 40.0 36.0 41.0 27.0 41.0 20 35.935173056598124 40.0 36.0 41.0 27.0 41.0 21 35.88840658106073 40.0 36.0 41.0 27.0 41.0 22 35.796251758028674 40.0 36.0 41.0 27.0 41.0 23 35.72929936835798 40.0 36.0 41.0 27.0 41.0 24 35.65043815483968 40.0 36.0 41.0 27.0 41.0 25 35.53797258723567 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.16681249975803425 1101 2 -0.18408823784260164 1101 3 0.2065007984486762 1101 4 0.08663070528157846 1101 5 -0.17473311814853787 1101 6 -0.0855035658869312 1101 7 -0.09122773435752407 1101 8 -0.13822725112873968 1101 9 -0.011134675341516242 1101 10 0.20100311393853332 1101 11 0.11460653632728679 1101 12 0.0014218477276770614 1101 13 0.14564095719367742 1101 14 -0.013916184196702375 1101 15 0.0160981594375329 1101 16 -0.10204346171458667 1101 17 0.037413624131446 1101 18 0.09323201951221449 1101 19 -0.0076762311260694105 1101 20 0.044746438865765015 1101 21 -0.13118814489035202 1101 22 -0.18253228615181172 1101 23 0.011470045929222294 1101 24 0.14166145485704362 1101 25 0.20364619201296819 1102 1 -0.4296207609535898 1102 2 -0.39569071892773877 1102 3 -0.12228660527295077 1102 4 -0.1911308651635153 1102 5 -0.445355434282412 1102 6 -0.25101920899237484 1102 7 -0.31406462345847075 1102 8 -0.3607805876429495 1102 9 0.025417706909180993 1102 10 -0.22940381128810827 1102 11 0.022526923485621353 1102 12 -0.23158340072164663 1102 13 -0.17652385774736246 1102 14 -0.14703458103400635 1102 15 -0.20516692133405456 1102 16 -0.3186776379806915 1102 17 -0.30013800834077387 1102 18 -0.2436638976031844 1102 19 -0.2267833131245638 1102 20 -0.1886377885216035 1102 21 -0.13642296187624225 1102 22 0.07711190988117522 1102 23 -0.11510073997494885 1102 24 -0.17120610699968353 1102 25 -0.19586577036178454 1103 1 -0.7391073541774524 1103 2 -0.8166939753539815 1103 3 -0.7490641290032904 1103 4 -0.6476596533572376 1103 5 -0.9693698457006903 1103 6 -1.0080038968604583 1103 7 -0.637731220839477 1103 8 -0.7704085236229972 1103 9 -0.6611325598406239 1103 10 -0.5618710738114885 1103 11 -0.6817826448646827 1103 12 -0.6371359538983938 1103 13 -0.6067157790388436 1103 14 -0.8939659189401894 1103 15 -0.7602046820213673 1103 16 -0.4993386232228403 1103 17 -0.5986164951418544 1103 18 -0.7175917019404636 1103 19 -0.4355437925813703 1103 20 -0.42451806289283667 1103 21 -0.483031428584475 1103 22 -0.755807095176678 1103 23 -0.6585664089280385 1103 24 -0.49221767770570324 1103 25 -0.6053773064249768 1104 1 -0.5274805150247097 1104 2 -0.29838928988154123 1104 3 -0.4401463593440731 1104 4 -0.5586416660940543 1104 5 -0.5582500836675877 1104 6 -0.39022824854039584 1104 7 -0.5177336164794113 1104 8 -0.5335555959513556 1104 9 -0.5770216417116245 1104 10 -0.5104606407995718 1104 11 -0.4017003945602369 1104 12 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2214 12 0.19139744479574716 2214 13 0.24813215494673102 2214 14 0.13447284274475635 2214 15 0.041159724931389974 2214 16 0.27348149156708246 2214 17 0.22370422219305652 2214 18 0.2549533527651704 2214 19 0.3740622042731445 2214 20 0.1122507981269294 2214 21 0.22857760482443013 2214 22 0.26677302288128857 2214 23 0.2593138582342789 2214 24 0.2009507557454171 2214 25 0.3397366324490463 2215 1 0.3667504072319936 2215 2 0.3449941747480416 2215 3 0.1648826849783589 2215 4 0.22246758830237923 2215 5 0.355555916173266 2215 6 0.2505770351390808 2215 7 0.2093929849369971 2215 8 0.10441687162244762 2215 9 -0.30936826381713445 2215 10 0.02309549829487878 2215 11 -0.010198842877258585 2215 12 0.13042157028083778 2215 13 0.1512529994744014 2215 14 0.13851637174147413 2215 15 0.0650843904582672 2215 16 5.270016216272211E-4 2215 17 0.2029329665280457 2215 18 0.21118282982018144 2215 19 0.09067595227173086 2215 20 0.3647949648614457 2215 21 0.21303851285449582 2215 22 0.162436405876754 2215 23 0.2515714500278179 2215 24 0.06151413602710676 2215 25 0.13201050690968685 2216 1 -0.7642660978418867 2216 2 -0.5191668062465169 2216 3 -2.407432414304779 2216 4 -1.8578686310914172 2216 5 -0.9641078644329397 2216 6 -0.8495838926562058 2216 7 -0.6533236358452967 2216 8 -0.6357658678740208 2216 9 -0.711913848942622 2216 10 -0.6653589298794529 2216 11 -0.6607647501243292 2216 12 -0.8466442239177852 2216 13 -0.8859811704174732 2216 14 -0.8739056580767155 2216 15 -0.8647936502125759 2216 16 -0.7552537641818802 2216 17 -1.163857388444093 2216 18 -1.0281553712464628 2216 19 -0.9721390871622262 2216 20 -0.9243962732185977 2216 21 -0.5117270778569107 2216 22 -0.7689870736215738 2216 23 -0.560717581612451 2216 24 -0.7334212933575159 2216 25 -0.7047076300518427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16408.0 3 13.0 4 234.0 5 1620.0 6 2317.0 7 1692.0 8 1339.0 9 1150.0 10 989.0 11 887.0 12 826.0 13 830.0 14 801.0 15 820.0 16 790.0 17 828.0 18 1036.0 19 1108.0 20 1520.0 21 1830.0 22 2444.0 23 2949.0 24 3488.0 25 4219.0 26 4988.0 27 5369.0 28 6292.0 29 7647.0 30 9740.0 31 12676.0 32 16503.0 33 22031.0 34 29757.0 35 41370.0 36 61814.0 37 105016.0 38 227474.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.156132837885124 21.197907120123975 11.23206638561645 27.413893656374448 2 13.48487019456653 22.488445633665773 41.03940980263443 22.987274369133264 3 17.769165975835904 26.675888841168856 30.129043856933112 25.425901326062135 4 11.482403069164384 19.435433536113447 39.850037033030134 29.232126361692035 5 12.132651566598756 39.44459979493735 35.94771568196647 12.475032956497422 6 28.53225426652794 40.26510173032571 19.208936703914066 11.993707299232284 7 26.095605923139033 33.13362022324141 23.01567351151583 17.75510034210373 8 23.501074495586327 38.95150537580712 21.019164473563748 16.528255655042805 9 25.452644790569074 16.80457830001648 21.104891077275457 36.63788583213899 10 13.493166531567443 31.334919015368023 35.21678847789505 19.955125975169487 11 32.472752814599296 24.30325767814699 25.338217964167537 17.885771543086175 12 21.994530744174977 26.590137030989673 32.71250788514896 18.70282433968639 13 27.64370944037496 23.689234420693296 26.56800035951286 22.09905577941889 14 19.790284863061007 24.00689064021371 29.043549179031814 27.159275317693464 15 21.761817576564578 32.33621837549933 24.36750998668442 21.534454061251665 16 20.42269461994767 30.485576186893738 28.758912937824142 20.332816255334446 17 20.28244967252815 30.309496267569884 28.52075930194819 20.88729475795378 18 19.72993260798285 29.766166843926477 30.669775787141756 19.834124760948914 19 21.691535664056325 28.70883716285379 28.943684828108484 20.6559423449814 20 21.76304734259635 29.124085404419354 29.48093580021704 19.631931452767255 21 21.663928463826593 28.3493047960996 28.48796272715016 21.498804012923653 22 21.176235613291944 29.53588042908383 28.96049532718058 20.327388630443647 23 20.906636662927294 29.268126242064934 28.786779269457767 21.03845782555 24 21.09551192962892 28.776079159142164 29.715136938991204 20.41327197223771 25 21.209266404125206 28.932046591677015 29.20568447754139 20.65300252665639 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 263.0 1 263.0 2 266.5 3 270.0 4 270.0 5 270.0 6 484.5 7 699.0 8 699.0 9 699.0 10 1175.0 11 1651.0 12 1651.0 13 1651.0 14 2752.5 15 3854.0 16 3854.0 17 3854.0 18 6847.0 19 9840.0 20 9840.0 21 9840.0 22 15185.5 23 20531.0 24 20531.0 25 20531.0 26 28317.0 27 36103.0 28 36103.0 29 36103.0 30 45971.0 31 55839.0 32 55839.0 33 55839.0 34 62558.5 35 69278.0 36 69278.0 37 69278.0 38 73089.5 39 76901.0 40 76901.0 41 76901.0 42 78376.0 43 79851.0 44 79851.0 45 79851.0 46 79179.0 47 78507.0 48 78507.0 49 78507.0 50 72723.0 51 66939.0 52 66939.0 53 66939.0 54 56202.5 55 45466.0 56 45466.0 57 45466.0 58 36959.0 59 28452.0 60 28452.0 61 28452.0 62 21809.0 63 15166.0 64 15166.0 65 15166.0 66 11112.5 67 7059.0 68 7059.0 69 7059.0 70 4909.0 71 2759.0 72 2759.0 73 2759.0 74 1850.5 75 942.0 76 942.0 77 942.0 78 625.0 79 308.0 80 308.0 81 308.0 82 200.0 83 92.0 84 92.0 85 92.0 86 63.5 87 35.0 88 35.0 89 35.0 90 22.0 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11401180063746744 2 0.10136231618717909 3 0.014813212053627157 4 0.0 5 0.0038281334520609504 6 0.3372086249511081 7 0.01997287018466583 8 0.011317959771310636 9 0.011317959771310636 10 0.003661692867188735 11 0.0031623711125720894 12 3.328811697444305E-4 13 3.328811697444305E-4 14 0.0 15 0.002496608773083229 16 4.993217546166457E-4 17 0.0 18 3.328811697444305E-4 19 0.0 20 4.993217546166457E-4 21 0.009320672752844053 22 0.0 23 1.6644058487221526E-4 24 0.0 25 0.00349525228231652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 600815.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.30991307582647 #Duplication Level Percentage of deduplicated Percentage of total 1 94.8932276985398 83.8001268954266 2 4.084600368567662 7.21421406995398 3 0.47059242434549153 1.246739282642784 4 0.15527263205295339 0.5484845055860441 5 0.10042004777869136 0.4434042845203289 6 0.05776535461626688 0.30607520669740923 7 0.03812349989036516 0.2356678073025099 8 0.02666847333394921 0.18840724495888408 9 0.021383123481793276 0.16995075983701455 >10 0.12779381875005327 2.1365125175683337 >50 0.011887652044296294 0.6833669993303834 >100 0.01037799789139393 1.6399994657929586 >500 0.001509526965798654 0.9046491667368971 >1k 3.773817414496635E-4 0.4824017936458495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1777 0.2957649193179265 No Hit GGTATCAACGCAGAGTACTTTTTTT 1118 0.18608057388713664 No Hit TATCAACGCAGAGTACTTTTTTTTT 966 0.1607816049865599 No Hit GTCCTACAGTGGACATTTCTAAATT 772 0.12849213152135017 No Hit CTGTAGGACGTGGAATATGGCAAGA 699 0.11634196882567846 No Hit CTTTAGGACGTGAAATATGGCGAGG 692 0.11517688473157293 No Hit GTCCTAAAGTGTGTATTTCTCATTT 611 0.1016951973569235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 1.6644058487221523E-4 8 0.0 0.0 0.0 0.0 1.6644058487221523E-4 9 0.0 0.0 0.0 0.0 1.6644058487221523E-4 10 0.0 0.0 0.0 0.0 1.6644058487221523E-4 11 0.0 0.0 0.0 0.0 1.6644058487221523E-4 12 0.0 0.0 0.0 0.0 1.6644058487221523E-4 13 0.0 0.0 0.0 0.0 1.6644058487221523E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 35 0.0021242208 16.33564 1 GTCTTAG 90 3.481182E-8 13.764291 1 TAGGACC 255 0.0 13.4371605 4 TAGGACT 115 3.6379788E-10 13.242419 4 TATACTT 105 1.9324943E-8 12.690651 5 GTAGGAC 520 0.0 12.2645855 3 GGCGAGG 225 0.0 12.231894 19 AGGACGT 455 0.0 12.132821 5 TTAGGAC 285 0.0 12.0237255 3 AGGACCT 415 0.0 11.926154 5 GGTATCA 400 0.0 11.911406 1 TGTAGCC 105 2.6062298E-7 11.796977 2 GGACGTG 460 0.0 11.793045 6 GGACCTG 385 0.0 11.618393 6 GACGTGA 265 0.0 11.461847 7 TAGGACG 490 0.0 11.460435 4 TGGCGAG 490 0.0 11.42705 18 AATACTG 100 1.8796145E-6 11.421586 5 GTCTAGA 60 0.005736297 11.1173115 1 TAGCCAG 60 0.0057864613 11.10432 5 >>END_MODULE