FastQCFastQC Report
Thu 26 May 2016
SRR1926797_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926797_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303984
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC7390.2431049002579083No Hit
CCCATGTACTCTGCGTTGATACCAC7110.23389388915206066No Hit
GTATCAACGCAGAGTACTTTTTTTT5970.19639191536396655No Hit
GTCCTACAGTGGACATTTCTAAATT4450.14638928364650772No Hit
GTCCTAAAGTGTGTATTTCTCATTT4170.13717827254066003No Hit
CTGTAGGACGTGGAATATGGCAAGA3910.12862519079951576No Hit
GGTATCAACGCAGAGTACTTTTTTT3840.12632243802305385No Hit
TATCAACGCAGAGTACTTTTTTTTT3540.11645349755250275No Hit
CTTTAGGACGTGAAATATGGCGAGG3220.10592662771724828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTG307.6230423E-419.0295495
GTCCTAG350.00210256116.3569411
GTCCTAT456.520657E-414.842411
GCTTCCT456.694615E-414.7909938
GTTCTAG400.0051157914.3123241
GTATAAT400.0051157914.3123241
CTTGCTC400.00516093314.2933953
ATATACT400.00517794814.286314
TATACTG400.005212119314.2721615
GTCCTAA1450.013.1607581
TAGGACC801.928378E-613.0957844
TGTAGGA2550.013.0789242
AGACCAG604.0220757E-412.6863665
CTGTGTA604.124073E-412.6487839
TAAAGTG1551.8189894E-1212.2771275
AAGGTGT701.069689E-412.2332816
GTACTGA550.002960248912.1437911
TAGACCA550.0030008912.1217174
TATTGTG550.003023258612.1097135
ACCATCC657.93108E-411.7027648