##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926797_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 303984 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.648445312911207 31.0 30.0 31.0 23.0 34.0 2 28.70080991104795 31.0 30.0 31.0 23.0 34.0 3 28.224702615927153 31.0 28.0 31.0 16.0 34.0 4 32.09550502658035 35.0 32.0 37.0 22.0 37.0 5 31.911041370598454 35.0 32.0 37.0 19.0 37.0 6 31.56372703826517 35.0 32.0 37.0 17.0 37.0 7 31.41242302226433 35.0 32.0 37.0 17.0 37.0 8 31.56310529501553 35.0 32.0 37.0 17.0 37.0 9 33.718136480867415 37.0 34.0 39.0 22.0 39.0 10 33.90827477761987 38.0 34.0 39.0 24.0 39.0 11 34.06777001421128 38.0 35.0 39.0 25.0 39.0 12 33.974393389125744 38.0 35.0 39.0 25.0 39.0 13 34.06745420811622 38.0 35.0 39.0 25.0 39.0 14 35.12440457392494 39.0 36.0 41.0 25.0 41.0 15 34.72282751723775 39.0 34.0 41.0 19.0 41.0 16 34.79492012737513 39.0 34.0 41.0 22.0 41.0 17 35.00961563766514 39.0 35.0 41.0 25.0 41.0 18 35.0718294383915 39.0 36.0 41.0 25.0 41.0 19 34.46130059476815 39.0 34.0 41.0 18.0 41.0 20 34.753848886783516 39.0 34.0 41.0 19.0 41.0 21 34.76772461708511 39.0 35.0 41.0 19.0 41.0 22 34.775958603084376 39.0 35.0 41.0 19.0 41.0 23 34.78693944418127 39.0 36.0 41.0 19.0 41.0 24 33.455089741565345 39.0 33.0 41.0 2.0 41.0 25 34.07150376335596 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.074057228737761 1101 2 -0.1608280713753345 1101 3 -1.0695587987680248 1101 4 -0.36021045398588214 1101 5 -0.38502088955029024 1101 6 -2.2568347308186283 1101 7 -0.6491494883104139 1101 8 0.10594524275071038 1101 9 0.11510643029988898 1101 10 -9.82083758381006 1101 11 -4.372797414315016 1101 12 -1.5462383728571751 1101 13 -0.6784839554760751 1101 14 -0.5546328814479651 1101 15 -1.6705012353120168 1101 16 -0.2711687136370955 1101 17 -0.3795886965591535 1101 18 -0.3644037529700128 1101 19 -0.9414585367801536 1101 20 -0.058981035896096046 1101 21 -0.05119454324081829 1101 22 0.0436838937443369 1101 23 -0.15032524760601262 1101 24 -0.6962793572443218 1101 25 -0.40594638160451524 1102 1 -0.46933701378120674 1102 2 -0.6645650346308969 1102 3 -1.0283006404420298 1102 4 -0.5444346907265327 1102 5 -0.6198006976342576 1102 6 -1.7765189124284113 1102 7 -0.5201792608719167 1102 8 0.04107648641587147 1102 9 -0.5278312301432422 1102 10 -0.4019235292286112 1102 11 -0.5249093432321956 1102 12 -0.9791624874005436 1102 13 -0.5825543562642039 1102 14 -0.7014117043755874 1102 15 -0.07051260250062796 1102 16 -0.2807076794123873 1102 17 -0.3990265890823821 1102 18 -0.4442108896699253 1102 19 -0.05103823498129856 1102 20 0.18306430024114206 1102 21 -0.349630607541215 1102 22 -0.21848610256132872 1102 23 -0.6991852133149976 1102 24 -4.089764063639311 1102 25 -2.0698565050332363 1103 1 0.3912887776766283 1103 2 0.34059733248909296 1103 3 0.08317058566867885 1103 4 0.36347236725791277 1103 5 0.22906421233969 1103 6 0.5221220847235521 1103 7 0.26585420718108566 1103 8 0.9183198600033791 1103 9 0.49912078992400666 1103 10 0.6092648340972318 1103 11 0.4357761259658446 1103 12 0.20040928842463757 1103 13 0.4236171954055621 1103 14 0.4950978755998605 1103 15 1.0555044663034394 1103 16 0.7816240220824682 1103 17 0.4294071856074595 1103 18 0.4222080092515057 1103 19 -1.0846324360516206 1103 20 0.18925040745729405 1103 21 0.20812337461368458 1103 22 0.2814095826895411 1103 23 0.3219057734054971 1103 24 1.9604622751070693 1103 25 1.143731582707595 1104 1 0.02936662522363065 1104 2 -0.14994189872445673 1104 3 -0.9086216966544498 1104 4 -0.2502664763948452 1104 5 -0.1488355063060851 1104 6 -1.0598013720205621 1104 7 -0.06307869635726959 1104 8 0.5441932692340359 1104 9 0.23128926707096298 1104 10 0.09672034845375066 1104 11 0.32519087190856766 1104 12 -0.039999513677138054 1104 13 -0.05275184444984404 1104 14 0.1508675005229989 1104 15 0.32625201144122684 1104 16 -0.1711559812714185 1104 17 -0.05548632833913558 1104 18 -0.22549364347166545 1104 19 -0.9565463901033056 1104 20 -0.12226089329359979 1104 21 -0.04304582920975264 1104 22 0.06946693423326167 1104 23 0.07892099634611327 1104 24 1.3589791097788506 1104 25 0.37543889978076805 1105 1 -0.3954383919206492 1105 2 -0.4697981710431094 1105 3 -0.04605129851633549 1105 4 0.017419667830282037 1105 5 -0.2665269803587087 1105 6 -0.5302163879212145 1105 7 -0.17275766308138074 1105 8 0.020573250401977106 1105 9 -0.6400531388131654 1105 10 -0.3387576482417529 1105 11 -0.8097248279866136 1105 12 -0.6492787433394014 1105 13 -0.5115656025112116 1105 14 -0.7246721445104853 1105 15 -0.04350032924134695 1105 16 -0.9895820832132571 1105 17 -0.7894346645446575 1105 18 -0.7613633824877795 1105 19 -1.4878191529082159 1105 20 -0.8897471689878103 1105 21 -0.9369289638589606 1105 22 -0.7622659703450623 1105 23 -0.7316702591835806 1105 24 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1108 22 0.09651742385059237 1108 23 0.471673965302287 1108 24 -0.5462400026595091 1108 25 0.42502567664027424 1109 1 0.3747737535922191 1109 2 0.3862070436110976 1109 3 -1.2081001087089263 1109 4 0.37281379019454874 1109 5 0.7330827782491163 1109 6 0.8236382107090812 1109 7 1.025446368841223 1109 8 1.3578704642499702 1109 9 0.7380264447006368 1109 10 0.9423448051329757 1109 11 0.26086169196072717 1109 12 0.33539892155902606 1109 13 0.3795141654023695 1109 14 0.18143834955226623 1109 15 -0.812910550135129 1109 16 0.21894833631768051 1109 17 0.22624360501351504 1109 18 0.4756074866355462 1109 19 -0.6354075897555802 1109 20 0.013207087001966045 1109 21 0.3840767439574435 1109 22 0.8064272355951871 1109 23 0.6089847459838964 1109 24 -0.6879638053182191 1109 25 0.05596628314791019 1110 1 0.5002100233116096 1110 2 0.507451017956388 1110 3 1.0327248824630253 1110 4 0.7341600877530823 1110 5 0.5839750268140484 1110 6 0.7277245695301495 1110 7 0.8099352865638352 1110 8 1.3600507863299427 1110 9 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0.8334416799030357 2215 2 0.24159142693457625 2215 3 0.8083692092969699 2215 4 0.9307223574948722 2215 5 1.1247341955472585 2215 6 1.790636063227506 2215 7 1.4944785731258676 2215 8 -0.15017762981051774 2215 9 0.5183561486181034 2215 10 0.3937446376214737 2215 11 0.26878474781509 2215 12 0.27351493319750375 2215 13 0.5445487196113916 2215 14 0.6632998707313575 2215 15 0.4216531856095287 2215 16 0.5940391378721301 2215 17 0.034216676150329306 2215 18 -0.5514404605546659 2215 19 0.6756914365342013 2215 20 -3.267247655121622 2215 21 -1.3710071507246582 2215 22 -0.394761222782968 2215 23 -0.436187786893214 2215 24 -0.9172957837402009 2215 25 -0.5824867362270609 2216 1 -0.8856705434900114 2216 2 -1.5269619375091992 2216 3 -0.5290160082252342 2216 4 -1.0956729081983383 2216 5 -0.750665661445062 2216 6 -1.8197197624536585 2216 7 -11.256739814650743 2216 8 -11.699665812860342 2216 9 -6.5785644821210205 2216 10 -4.212466787939267 2216 11 -2.762074803592405 2216 12 -2.2630319959007963 2216 13 -2.2531800926722134 2216 14 -2.052510004325093 2216 15 -3.0580199665807513 2216 16 -2.1406533956217757 2216 17 -2.3923883968588555 2216 18 -2.2310891509054116 2216 19 -1.6580856596572815 2216 20 -2.298751681916741 2216 21 -4.9303882661845435 2216 22 -3.464651520841077 2216 23 -3.404716689497455 2216 24 -4.2745656542806145 2216 25 -3.386580330988462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14736.0 3 1.0 4 106.0 5 643.0 6 878.0 7 732.0 8 590.0 9 518.0 10 479.0 11 492.0 12 464.0 13 448.0 14 463.0 15 547.0 16 496.0 17 616.0 18 662.0 19 791.0 20 1090.0 21 1389.0 22 1726.0 23 2155.0 24 2450.0 25 2794.0 26 3270.0 27 3561.0 28 4067.0 29 4961.0 30 6021.0 31 7284.0 32 9539.0 33 12891.0 34 17504.0 35 25131.0 36 37578.0 37 60747.0 38 76164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40369240913212 19.694387208439792 12.675842742932497 25.226077639495593 2 14.024500713149287 21.469252230856142 41.64363617198592 22.862610884008657 3 18.409618710368115 26.174750885283704 30.16816272749732 25.24746767685086 4 11.405293304365001 18.404108236223582 39.1395088550925 31.051089604318914 5 11.948263892316247 38.80142271005867 36.48705116689205 12.763262230733039 6 29.418228957602636 39.40789776727842 18.965959714938112 12.20791356018083 7 25.879478182873424 32.58949770484884 23.685564290613623 17.845459821664118 8 23.607186544846666 37.58098711130308 22.515488527444123 16.296337816406133 9 25.919114018174998 15.948528298907 20.997979824567174 37.134377858350824 10 13.83677807649403 30.154034479241048 35.5714017762831 20.43778566798182 11 32.316798325990895 24.062564774084443 25.369726361366183 18.250910538558472 12 21.921832999384744 26.40595645836829 32.73781910185926 18.93439144038771 13 27.883609372840873 23.5380434424989 26.22671430733495 22.35163287732528 14 19.816476332421967 23.692583051204352 28.578102843004682 27.912837773369 15 22.426647189266376 32.037691401649 23.715708918148856 21.81995249093577 16 20.723192840456683 29.676241239759154 28.856973645247265 20.7435922745369 17 20.626846618015755 30.010462797844266 28.703271104910932 20.659419479229044 18 20.48903906324541 28.954771124849067 30.49552054168051 20.06066927022501 19 22.666162147933665 28.4357725717294 28.27059752566465 20.62746775467228 20 22.199812459900965 28.781811900570847 28.922960501423 20.09541513810519 21 21.778454816442828 28.20641051793458 28.197198112773 21.817936552849595 22 21.624031454093803 29.370753615082172 28.13430503232599 20.870909898498034 23 21.020422264117418 29.100821562647035 28.582944267322073 21.295811905913475 24 22.141649173833795 28.456507004810256 28.774668184540065 20.627175636815885 25 21.68870522509599 28.275925603003255 28.644752037428812 21.390617134471945 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 371.0 1 371.0 2 414.5 3 458.0 4 458.0 5 458.0 6 533.5 7 609.0 8 609.0 9 609.0 10 830.0 11 1051.0 12 1051.0 13 1051.0 14 1651.5 15 2252.0 16 2252.0 17 2252.0 18 3628.5 19 5005.0 20 5005.0 21 5005.0 22 7487.5 23 9970.0 24 9970.0 25 9970.0 26 13664.5 27 17359.0 28 17359.0 29 17359.0 30 21966.5 31 26574.0 32 26574.0 33 26574.0 34 29954.0 35 33334.0 36 33334.0 37 33334.0 38 35049.0 39 36764.0 40 36764.0 41 36764.0 42 37489.5 43 38215.0 44 38215.0 45 38215.0 46 38686.0 47 39157.0 48 39157.0 49 39157.0 50 36432.0 51 33707.0 52 33707.0 53 33707.0 54 28986.5 55 24266.0 56 24266.0 57 24266.0 58 20087.0 59 15908.0 60 15908.0 61 15908.0 62 12924.5 63 9941.0 64 9941.0 65 9941.0 66 7596.5 67 5252.0 68 5252.0 69 5252.0 70 3812.0 71 2372.0 72 2372.0 73 2372.0 74 1665.5 75 959.0 76 959.0 77 959.0 78 642.0 79 325.0 80 325.0 81 325.0 82 208.5 83 92.0 84 92.0 85 92.0 86 61.5 87 31.0 88 31.0 89 31.0 90 21.0 91 11.0 92 11.0 93 11.0 94 6.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21678772566977206 2 0.1319148376230328 3 0.1345465550818464 4 0.06710879519974736 5 0.018750986894047056 6 0.016448234117585135 7 0.24211800621085322 8 0.22863045423443337 9 0.016448234117585135 10 0.02993578609400495 11 0.014145481341123218 12 0.014803410705826624 13 0.013816516658771516 14 0.014803410705826624 15 0.013816516658771516 16 0.017764092846991947 17 0.016448234117585135 18 0.013487551976419812 19 0.021053739670508973 20 0.016119269435233435 21 0.015132375388178326 22 0.016119269435233435 23 0.01677719879993684 24 0.016448234117585135 25 0.015461340070530027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 303984.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.48525189725275 #Duplication Level Percentage of deduplicated Percentage of total 1 92.13806631744862 78.76445808472874 2 6.196365085496456 10.593956603620132 3 0.9505450210810334 2.4377274170037473 4 0.27075281880176044 0.9258149166863887 5 0.12701903050789726 0.5429126909356214 6 0.07022653142278922 0.3601999637128483 7 0.04027004909609562 0.24097467036261286 8 0.02734850448511199 0.18703150359383564 9 0.02511500055173077 0.19322659337079118 >10 0.12459035981573618 2.0117733833506923 >50 0.017357891664629137 1.079578757598798 >100 0.011186196850494504 1.9873613842483313 >500 0.0011571927776373625 0.6749840307875119 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 739 0.2431049002579083 No Hit CCCATGTACTCTGCGTTGATACCAC 711 0.23389388915206066 No Hit GTATCAACGCAGAGTACTTTTTTTT 597 0.19639191536396655 No Hit GTCCTACAGTGGACATTTCTAAATT 445 0.14638928364650772 No Hit GTCCTAAAGTGTGTATTTCTCATTT 417 0.13717827254066003 No Hit CTGTAGGACGTGGAATATGGCAAGA 391 0.12862519079951576 No Hit GGTATCAACGCAGAGTACTTTTTTT 384 0.12632243802305385 No Hit TATCAACGCAGAGTACTTTTTTTTT 354 0.11645349755250275 No Hit CTTTAGGACGTGAAATATGGCGAGG 322 0.10592662771724828 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 3.289646823517027E-4 13 0.0 0.0 0.0 0.0 3.289646823517027E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAGTG 30 7.6230423E-4 19.029549 5 GTCCTAG 35 0.002102561 16.356941 1 GTCCTAT 45 6.520657E-4 14.84241 1 GCTTCCT 45 6.694615E-4 14.790993 8 GTTCTAG 40 0.00511579 14.312324 1 GTATAAT 40 0.00511579 14.312324 1 CTTGCTC 40 0.005160933 14.293395 3 ATATACT 40 0.005177948 14.28631 4 TATACTG 40 0.0052121193 14.272161 5 GTCCTAA 145 0.0 13.160758 1 TAGGACC 80 1.928378E-6 13.095784 4 TGTAGGA 255 0.0 13.078924 2 AGACCAG 60 4.0220757E-4 12.686366 5 CTGTGTA 60 4.124073E-4 12.648783 9 TAAAGTG 155 1.8189894E-12 12.277127 5 AAGGTGT 70 1.069689E-4 12.233281 6 GTACTGA 55 0.0029602489 12.143791 1 TAGACCA 55 0.00300089 12.121717 4 TATTGTG 55 0.0030232586 12.109713 5 ACCATCC 65 7.93108E-4 11.702764 8 >>END_MODULE