FastQCFastQC Report
Thu 26 May 2016
SRR1926797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303984
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11650.3832438549397337No Hit
GTACATGGGAAGCAGTGGTATCAAC8510.27994894468129905No Hit
GGTATCAACGCAGAGTACTTTTTTT8440.2776461919048371No Hit
TATCAACGCAGAGTACTTTTTTTTT8040.264487604610769No Hit
CCCATGTACTCTGCGTTGATACCAC6990.2299463129638402No Hit
CTGTAGGACGTGGAATATGGCAAGA4540.14934996578767304No Hit
GTCCTACAGTGGACATTTCTAAATT4270.14046791936417707No Hit
CTTTAGGACGTGAAATATGGCGAGG4150.13652034317595663No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3970.13059897889362598No Hit
GTCCTAAAGTGTGTATTTCTCATTT3840.12632243802305385No Hit
GAGTACATGGGAAGCAGTGGTATCA3710.12204589715248172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAATA350.002183802416.26442312
ACGTGAA1351.8189894E-1213.3528918
AAGTGTG1600.013.04542357
TGGCGAG2350.012.91925718
CGTGAAA1403.6379788E-1212.8760019
GTCCTAA1352.5465852E-1112.7128451
AAAGTGT1600.012.49974256
GGCGAGG1155.355105E-912.37510419
TAGGACC853.80665E-612.3246754
GGCGAGA1405.2750693E-1112.19831819
TGTCCAC701.09867906E-412.19831810
GGACGTG2900.012.1508176
GTACTGA550.00297638712.1349891
TAGGACT550.003002299512.1209624
TATGGCG2350.012.11180416
GACGTGA1501.4551915E-1112.0176027
CTAAAGT1750.011.9725424
CTGTAGG2600.011.7349341
GTGAAAT1800.011.59593210
TAAAGTG1655.456968E-1211.5437745
Produced by FastQC (version 0.11.5)