##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926797_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 303984 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.871851807989895 31.0 30.0 33.0 26.0 34.0 2 30.212514474446024 31.0 30.0 34.0 26.0 34.0 3 30.324299963155955 31.0 30.0 34.0 26.0 34.0 4 33.880727275119746 37.0 35.0 37.0 30.0 37.0 5 33.84551818516764 37.0 35.0 37.0 30.0 37.0 6 33.76948457813569 37.0 35.0 37.0 29.0 37.0 7 33.74394047055108 37.0 35.0 37.0 29.0 37.0 8 33.756365466603505 37.0 35.0 37.0 29.0 37.0 9 35.287307884625505 39.0 35.0 39.0 29.0 39.0 10 35.188381625348704 39.0 35.0 39.0 29.0 39.0 11 35.22731788515185 39.0 35.0 39.0 29.0 39.0 12 35.15449826306648 39.0 35.0 39.0 29.0 39.0 13 35.15308700457919 39.0 35.0 39.0 29.0 39.0 14 36.25860900573714 40.0 36.0 41.0 29.0 41.0 15 36.23572293278593 40.0 36.0 41.0 29.0 41.0 16 36.20013224380231 40.0 36.0 41.0 29.0 41.0 17 36.190302779093635 40.0 36.0 41.0 29.0 41.0 18 36.15129085741355 40.0 36.0 41.0 29.0 41.0 19 36.20866558766251 40.0 37.0 41.0 29.0 41.0 20 36.18911850623717 40.0 37.0 41.0 29.0 41.0 21 36.13816516658772 40.0 37.0 41.0 29.0 41.0 22 36.047048528869944 40.0 37.0 41.0 28.0 41.0 23 35.994802358018845 40.0 37.0 41.0 28.0 41.0 24 35.93742104847624 40.0 37.0 41.0 28.0 41.0 25 35.82156955629244 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2144165062804788 1101 2 0.3789408503270657 1101 3 0.5087900753935699 1101 4 0.3703836619898908 1101 5 0.27006299461682204 1101 6 0.3923969405049732 1101 7 0.2789485414473063 1101 8 0.3641754909771322 1101 9 0.7247808672672917 1101 10 0.09530485473632666 1101 11 0.38983359596148404 1101 12 0.3312318675748642 1101 13 0.5264405977069231 1101 14 0.5159388529976923 1101 15 0.6382458452547226 1101 16 0.7180493999723012 1101 17 0.5764363897057976 1101 18 0.7246578762447484 1101 19 0.8807574612182592 1101 20 0.783235743210092 1101 21 0.7428135380893934 1101 22 0.5907817710825114 1101 23 0.7862550814831337 1101 24 0.9521574024557466 1101 25 0.6815646972112503 1102 1 -0.6800296748177814 1102 2 -0.5821028470404848 1102 3 -0.44289463668501483 1102 4 -0.4151837975381838 1102 5 -0.3788329671894246 1102 6 -0.5711238567138537 1102 7 -0.4278585099986927 1102 8 -0.4093759029243742 1102 9 -0.3783716330405724 1102 10 -0.14414970966353025 1102 11 -0.12670092713759118 1102 12 -0.278437820869442 1102 13 -0.15982499665494743 1102 14 -0.27422284526028307 1102 15 -0.10530090704476436 1102 16 -0.28534654762496103 1102 17 -0.034862595772615634 1102 18 -0.11283868991702661 1102 19 -0.20394230291257998 1102 20 -0.08364026918014389 1102 21 -0.14703366795037454 1102 22 -0.1772518000145169 1102 23 -0.08096668289399389 1102 24 -0.5485246288608607 1102 25 -0.6416897288878118 1103 1 -0.5737790380126278 1103 2 -0.6894257424297727 1103 3 -0.6509079501581141 1103 4 -0.9307461025545081 1103 5 -0.8740611738975943 1103 6 -0.6730660517872877 1103 7 -0.45724615816202885 1103 8 -0.6112758869132691 1103 9 -0.5929786090287692 1103 10 -0.5644459635510728 1103 11 -0.5204187533152549 1103 12 -0.6837612693694908 1103 13 -0.5391279800567759 1103 14 -0.6823506623649962 1103 15 -0.3612262273880802 1103 16 -0.5218935838731227 1103 17 -0.2661356723784607 1103 18 -0.41862160849814956 1103 19 -0.6255776670680859 1103 20 -0.7452723340784715 1103 21 -0.6484334263283031 1103 22 -1.0109498306490892 1103 23 -0.9031124615429462 1103 24 -0.5684317644748802 1103 25 -0.6066836398175752 1104 1 -0.46555548251503964 1104 2 -0.5114971430521074 1104 3 -0.39756337507752804 1104 4 -0.37286818420313494 1104 5 -0.7242074408300851 1104 6 -0.5338190004168553 1104 7 -0.8029205698335673 1104 8 -0.6891094593666409 1104 9 -0.6000217810647683 1104 10 -0.8235899759232055 1104 11 -0.46151603479991365 1104 12 -0.2962191128172904 1104 13 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1105 24 0.019357644074595726 1105 25 0.04308689594274995 1106 1 0.36348482926184644 1106 2 0.21170953092182998 1106 3 0.4815926351526514 1106 4 0.37414801358033145 1106 5 0.34478537368702433 1106 6 0.5480528787696031 1106 7 0.3970550725973183 1106 8 0.3578702020631468 1106 9 0.4216564554472271 1106 10 0.6033982106557687 1106 11 0.36837679189598305 1106 12 0.3436542278233148 1106 13 0.4825859016783127 1106 14 0.11352966797981168 1106 15 0.14624509238440453 1106 16 0.5592878716455587 1106 17 0.3543396458699206 1106 18 0.38944236711619595 1106 19 0.20119789035434366 1106 20 0.4471732549736913 1106 21 0.4027984806830318 1106 22 0.3658617635538093 1106 23 0.28634919027289385 1106 24 0.24285945402736075 1106 25 0.45269212051258734 1107 1 0.5526895748440026 1107 2 0.5548493125425793 1107 3 0.6906980705795718 1107 4 0.8372245665901303 1107 5 0.7725148995583524 1107 6 0.7333015407456713 1107 7 0.6172126286740536 1107 8 0.7418766280143672 1107 9 0.333832258476356 1107 10 0.5947300491822674 1107 11 0.52723008605507 1107 12 0.3633401465711117 1107 13 0.5233150925067278 1107 14 0.41721599908811413 1107 15 0.3004936942245635 1107 16 0.6292688858805846 1107 17 0.7226076458355593 1107 18 0.5772382143022625 1107 19 0.6252204670920563 1107 20 0.6580269271299102 1107 21 0.839578460580455 1107 22 1.0284692564878384 1107 23 0.9901583704486612 1107 24 0.8136651892747366 1107 25 0.7892599065662154 1108 1 0.8362189462647365 1108 2 0.6137893351755501 1108 3 0.7774835031779084 1108 4 0.9026544215333772 1108 5 0.7349390276746703 1108 6 1.006131690603361 1108 7 0.7997080849341245 1108 8 0.5312357034918875 1108 9 0.7012665028438363 1108 10 0.5169892309661606 1108 11 0.6915848749854874 1108 12 0.7207438707232328 1108 13 0.7705374099855575 1108 14 0.8131643547686522 1108 15 0.699245451708876 1108 16 0.6734803326759646 1108 17 0.9132132492099316 1108 18 0.884240717645774 1108 19 0.7086795391403342 1108 20 0.7495875731982906 1108 21 0.8479886659917923 1108 22 0.672934466871574 1108 23 0.8395805438995012 1108 24 0.8479661391735718 1108 25 0.8179283335748906 1109 1 0.5785621645437828 1109 2 0.1489635929664388 1109 3 0.25463888513690236 1109 4 0.5140486514966653 1109 5 0.5097827069531675 1109 6 0.4285832018433595 1109 7 0.3277661876280362 1109 8 0.39456194022767477 1109 9 0.2133656428703219 1109 10 0.5197887548794569 1109 11 0.25342166441770786 1109 12 0.2505789167393573 1109 13 0.28316460392382936 1109 14 0.4811399804706227 1109 15 0.5428409121215907 1109 16 0.645883229428847 1109 17 0.5408472324127871 1109 18 0.41342617353231503 1109 19 0.5119710751904947 1109 20 0.37756940143827933 1109 21 0.45091307884613485 1109 22 0.5245471267545625 1109 23 0.3403851974793781 1109 24 0.5404585536372224 1109 25 0.3527798917530163 1110 1 0.5028298473253976 1110 2 0.28999470874934374 1110 3 0.4903436095786837 1110 4 0.6307636328961763 1110 5 0.5759150373109705 1110 6 0.6326917334313436 1110 7 0.43495919745796385 1110 8 0.4793235008619803 1110 9 0.3662797192924572 1110 10 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0.13838696786159232 2215 24 0.4628316597053441 2215 25 0.5088196340552287 2216 1 -1.5279140798001087 2216 2 -1.4412805948943799 2216 3 -2.7110733944698993 2216 4 -2.744615921759692 2216 5 -1.7198845578307314 2216 6 -1.5230521936941628 2216 7 -1.6275408025420361 2216 8 -1.8125540295023903 2216 9 -1.8889075276711011 2216 10 -1.8171056514241783 2216 11 -1.4609302235523387 2216 12 -1.435823993230997 2216 13 -1.6681148468485176 2216 14 -1.6599165416077 2216 15 -1.3914848407259655 2216 16 -1.9672340665611685 2216 17 -1.987374799105389 2216 18 -1.5848867457998708 2216 19 -1.7137417895309923 2216 20 -1.7540082627839126 2216 21 -1.7982287529528946 2216 22 -2.246526753726009 2216 23 -2.3174641159860627 2216 24 -2.4095433967450504 2216 25 -2.328269218872663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7407.0 3 9.0 4 120.0 5 826.0 6 1127.0 7 806.0 8 633.0 9 563.0 10 474.0 11 409.0 12 415.0 13 372.0 14 360.0 15 389.0 16 392.0 17 417.0 18 489.0 19 587.0 20 718.0 21 959.0 22 1125.0 23 1427.0 24 1661.0 25 1906.0 26 2378.0 27 2606.0 28 2903.0 29 3641.0 30 4738.0 31 6072.0 32 7843.0 33 10552.0 34 14445.0 35 20066.0 36 29898.0 37 52860.0 38 122391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.379003734513624 20.456835740678535 10.58507380110257 27.57908672370527 2 13.891999051583328 22.373412719321355 39.819010485273196 23.91557774382212 3 17.769530427458413 26.422667930090544 30.181024952621605 25.626776689829438 4 11.991091636401915 18.615453444918153 38.48952576451392 30.90392915416601 5 12.253142569521435 39.371518993588204 35.873158952663246 12.502179484227113 6 29.69914635998125 39.12087621889628 18.581359882220124 12.59861753890235 7 26.432874765149695 33.20873791199571 22.94442232583238 17.413964997022212 8 24.41322585951637 37.882875472939624 20.908373087678893 16.79552557986511 9 26.108155590868265 15.923619267579314 20.705311053426374 37.262914088126045 10 14.203281881225902 30.396549682207574 34.75451686339531 20.645651573171214 11 32.537750435898275 24.00072375563378 25.056749021284997 18.404776787182946 12 22.235709774198643 26.522119585241327 32.274396020843206 18.967774619716828 13 27.960774122237098 23.222022284140888 26.428780556807453 22.388423036814558 14 20.314951822963785 23.757249583035893 28.355533039676562 27.57226555432376 15 22.335315945995738 31.583414480090532 24.183159196778693 21.898110377135037 16 20.667206168745725 29.694326017158794 28.647889362598033 20.990578451497445 17 20.68727301436918 29.77623822306437 28.61236117690405 20.924127585662404 18 20.5474630243697 28.52058003052792 30.657534607084585 20.27442233801779 19 22.081169012740844 28.170654280009078 29.056230118131605 20.69194658911847 20 22.24835681060063 28.375035363936025 29.26028514846274 20.11632267700061 21 21.748600080277416 27.844090726642236 28.413599784172188 21.99370940890816 22 21.851807989894205 28.84658403073846 28.40478446234012 20.89682351702721 23 21.04525582022679 28.73154090853765 28.950303141952016 21.272900129283546 24 21.813319122059056 28.090294226011896 29.376546134007054 20.719840517921995 25 21.360919298222527 28.124064466676096 29.042573140200872 21.472443094900502 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 174.0 1 174.0 2 182.5 3 191.0 4 191.0 5 191.0 6 344.5 7 498.0 8 498.0 9 498.0 10 696.0 11 894.0 12 894.0 13 894.0 14 1402.5 15 1911.0 16 1911.0 17 1911.0 18 3281.5 19 4652.0 20 4652.0 21 4652.0 22 7068.5 23 9485.0 24 9485.0 25 9485.0 26 13390.5 27 17296.0 28 17296.0 29 17296.0 30 21838.0 31 26380.0 32 26380.0 33 26380.0 34 29807.0 35 33234.0 36 33234.0 37 33234.0 38 34822.0 39 36410.0 40 36410.0 41 36410.0 42 37262.0 43 38114.0 44 38114.0 45 38114.0 46 39261.5 47 40409.0 48 40409.0 49 40409.0 50 37433.5 51 34458.0 52 34458.0 53 34458.0 54 29426.0 55 24394.0 56 24394.0 57 24394.0 58 20349.5 59 16305.0 60 16305.0 61 16305.0 62 13191.5 63 10078.0 64 10078.0 65 10078.0 66 7680.0 67 5282.0 68 5282.0 69 5282.0 70 3814.0 71 2346.0 72 2346.0 73 2346.0 74 1653.0 75 960.0 76 960.0 77 960.0 78 658.0 79 356.0 80 356.0 81 356.0 82 238.0 83 120.0 84 120.0 85 120.0 86 75.5 87 31.0 88 31.0 89 31.0 90 18.0 91 5.0 92 5.0 93 5.0 94 3.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10855834517606189 2 0.10526869835254488 3 0.01579030475288173 4 0.0 5 0.004276540870572136 6 0.34409705773988103 7 0.02204063371756408 8 0.011184799199957893 9 0.01085583451760619 10 0.003947576188220433 11 0.004605505552923838 12 0.0 13 3.289646823517027E-4 14 3.289646823517027E-4 15 0.0026317174588136216 16 0.0 17 0.0 18 0.0 19 3.289646823517027E-4 20 6.579293647034054E-4 21 0.012500657929364703 22 0.0 23 3.289646823517027E-4 24 0.0 25 0.004276540870572136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 303984.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.80305052605783 #Duplication Level Percentage of deduplicated Percentage of total 1 90.55891355700561 74.98554294845638 2 7.387964418520193 12.2349198206289 3 1.2034341337857413 2.989440521539301 4 0.34172583054688566 1.131837648513314 5 0.14421595660677455 0.5970760570787259 6 0.08647272179553048 0.4296123091176631 7 0.0521356845151659 0.3021895603383925 8 0.03872791090245385 0.25654313305796417 9 0.022618999205467828 0.16856299206532932 >10 0.1287919409105083 2.015856974110401 >50 0.019090279748588655 1.1545940198777895 >100 0.013919995649971768 2.2970032198033272 >500 0.0015908566457110591 1.0531636268001492 >1k 3.977141614277648E-4 0.383657168612312 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1165 0.3832438549397337 No Hit GTACATGGGAAGCAGTGGTATCAAC 851 0.27994894468129905 No Hit GGTATCAACGCAGAGTACTTTTTTT 844 0.2776461919048371 No Hit TATCAACGCAGAGTACTTTTTTTTT 804 0.264487604610769 No Hit CCCATGTACTCTGCGTTGATACCAC 699 0.2299463129638402 No Hit CTGTAGGACGTGGAATATGGCAAGA 454 0.14934996578767304 No Hit GTCCTACAGTGGACATTTCTAAATT 427 0.14046791936417707 No Hit CTTTAGGACGTGAAATATGGCGAGG 415 0.13652034317595663 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 397 0.13059897889362598 No Hit GTCCTAAAGTGTGTATTTCTCATTT 384 0.12632243802305385 No Hit GAGTACATGGGAAGCAGTGGTATCA 371 0.12204589715248172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.289646823517027E-4 0.0 10 0.0 0.0 0.0 3.289646823517027E-4 0.0 11 0.0 0.0 0.0 3.289646823517027E-4 0.0 12 0.0 0.0 0.0 3.289646823517027E-4 0.0 13 0.0 0.0 0.0 3.289646823517027E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCAATA 35 0.0021838024 16.264423 12 ACGTGAA 135 1.8189894E-12 13.352891 8 AAGTGTG 160 0.0 13.0454235 7 TGGCGAG 235 0.0 12.919257 18 CGTGAAA 140 3.6379788E-12 12.876001 9 GTCCTAA 135 2.5465852E-11 12.712845 1 AAAGTGT 160 0.0 12.4997425 6 GGCGAGG 115 5.355105E-9 12.375104 19 TAGGACC 85 3.80665E-6 12.324675 4 GGCGAGA 140 5.2750693E-11 12.198318 19 TGTCCAC 70 1.09867906E-4 12.198318 10 GGACGTG 290 0.0 12.150817 6 GTACTGA 55 0.002976387 12.134989 1 TAGGACT 55 0.0030022995 12.120962 4 TATGGCG 235 0.0 12.111804 16 GACGTGA 150 1.4551915E-11 12.017602 7 CTAAAGT 175 0.0 11.972542 4 CTGTAGG 260 0.0 11.734934 1 GTGAAAT 180 0.0 11.595932 10 TAAAGTG 165 5.456968E-12 11.543774 5 >>END_MODULE