FastQCFastQC Report
Thu 26 May 2016
SRR1926796_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926796_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769855
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14860.19302336154210858No Hit
CTGTAGGACGTGGAATATGGCAAGA11190.14535204681401043No Hit
GTCCTAAAGTGTGTATTTCTCATTT10310.13392132284651004No Hit
GTCCTACAGTGTGCATTTCTCATTT9920.12885543381545875No Hit
GTATCAACGCAGAGTACTTTTTTTT8680.11274850458852641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA250.00609804918.96277617
CCCCTAC706.884973E-613.6396511
TAGGACC2550.013.0714134
GTCCTAC8150.013.0036921
GTATTAG751.4020699E-512.7303421
CCTATAC1001.368553E-712.3974843
GACGTGA2550.012.3164397
GTATCAA6450.011.9902051
GGACGTG5400.011.8084386
TCCTACA9400.011.7684792
CCCTATA657.761311E-411.7372632
AGGACGT5600.011.7266085
GCCTTAG907.042263E-611.6694791
GTTTTAG1251.6947524E-811.4573071
TTAGGAC3350.011.3868973
CCTACAG8800.011.378813
GTCTAGA600.00565591311.1390491
GTACTAG600.00565591311.1390491
TGTAGGA7900.011.1058282
GGACCTG4200.011.1034566
Produced by FastQC (version 0.11.5)