FastQCFastQC Report
Thu 26 May 2016
SRR1926796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769855
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15370.1996479856596372No Hit
GTATCAACGCAGAGTACTTTTTTTT15260.19821914516369965No Hit
CTGTAGGACGTGGAATATGGCAAGA13620.17691643231517623No Hit
GTCCTAAAGTGTGTATTTCTCATTT10200.1324924823505725No Hit
GTCCTACAGTGTGCATTTCTCATTT10190.13236258776003273No Hit
GGTATCAACGCAGAGTACTTTTTTT9700.12599775282358366No Hit
CTTTAGGACGTGAAATATGGCGAGG9480.12314007183170857No Hit
TATCAACGCAGAGTACTTTTTTTTT8630.11209903163582752No Hit
CTGTAGGACCTGGAATATGGCGAGA8200.10651356424261711No Hit
CACACACACACACACACACACACAC7860.10209714816426471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA351.00274956E-418.9761619
CGTGCGC350.002188075816.26422310
GTGCGCC456.8248285E-414.75827611
CGTTTCC456.8315543E-414.75635914
GTCTTAG801.2297278E-714.3017991
GGTTATA801.2297278E-714.3017991
GACCTAT400.00515037914.3017991
GTTGGAC400.00518842814.2859453
TAGACAG1006.330083E-1014.2850145
CTAAACT602.5062423E-514.2850134
TCCAACG801.3053977E-714.232120518
GCGTGCG400.00532231914.2311959
GGCGAGG2300.013.61333319
TAGGACC2750.013.5058314
TGTAGGA8150.013.1018142
GTAGGAC7650.012.6986173
CTACTAA550.002983801512.1348591
CACTAGG550.002983801512.1348591
TGGCGAG6500.012.11554818
CCGTTTC550.003099511112.07338513