##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926796_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 769855 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.11940300446188 31.0 30.0 33.0 26.0 34.0 2 30.401935429399042 31.0 30.0 34.0 27.0 34.0 3 30.481840086769587 31.0 31.0 34.0 26.0 34.0 4 34.081433516701196 37.0 35.0 37.0 30.0 37.0 5 34.130516785628465 37.0 35.0 37.0 30.0 37.0 6 34.097089711698956 37.0 35.0 37.0 30.0 37.0 7 34.10583811237181 37.0 35.0 37.0 30.0 37.0 8 34.140803138253304 37.0 35.0 37.0 30.0 37.0 9 35.68394567808224 39.0 37.0 39.0 30.0 39.0 10 35.5920829247066 39.0 35.0 39.0 30.0 39.0 11 35.63366867786791 39.0 37.0 39.0 30.0 39.0 12 35.53509427099908 39.0 35.0 39.0 30.0 39.0 13 35.556157977801014 39.0 35.0 39.0 30.0 39.0 14 36.691054809022475 40.0 37.0 41.0 30.0 41.0 15 36.69122626988199 40.0 37.0 41.0 30.0 41.0 16 36.6800865097973 40.0 37.0 41.0 30.0 41.0 17 36.655863766553445 40.0 37.0 41.0 30.0 41.0 18 36.62575160257451 40.0 37.0 41.0 30.0 41.0 19 36.705244494093044 40.0 37.0 41.0 30.0 41.0 20 36.67381519896604 40.0 37.0 41.0 30.0 41.0 21 36.63156568444707 40.0 37.0 41.0 30.0 41.0 22 36.545426086730615 40.0 37.0 41.0 30.0 41.0 23 36.48709692084873 40.0 37.0 41.0 30.0 41.0 24 36.42734800709224 40.0 37.0 41.0 30.0 41.0 25 36.313647375155064 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.15547804235842833 1101 2 -0.32937336533378314 1101 3 -0.05433558271673533 1101 4 -0.16645209561416152 1101 5 -0.08951555522230592 1101 6 -0.1401573814842081 1101 7 -0.18552739926406758 1101 8 -0.15890248503308158 1101 9 -0.3419013430877911 1101 10 -0.14163748613496097 1101 11 -0.04653889744949424 1101 12 0.10096113078025581 1101 13 -0.015707285067811938 1101 14 -0.05435246694991491 1101 15 -0.11775703760676492 1101 16 -0.07466997207085768 1101 17 -0.08529032478376308 1101 18 -0.05250426945205788 1101 19 -0.2634358710926037 1101 20 -0.19446586508575336 1101 21 -0.13318627944757822 1101 22 -0.04088947172134283 1101 23 -0.056616614186069114 1101 24 -0.016111639237117004 1101 25 -0.10143541480459106 1102 1 -1.0528440057170236 1102 2 -0.8699518095066701 1102 3 -0.5341354163760919 1102 4 -0.8257550136999114 1102 5 -0.8711648056826888 1102 6 -0.7492785484087534 1102 7 -0.6447863315553661 1102 8 -0.7406719220304012 1102 9 -0.6238262791612499 1102 10 -0.6917650294508988 1102 11 -0.5459944958034342 1102 12 -0.6756072957828252 1102 13 -0.5380742164236381 1102 14 -0.5488240411302527 1102 15 -0.7541865681823126 1102 16 -0.6067308149563857 1102 17 -0.5323462967071961 1102 18 -0.6186053159732339 1102 19 -0.6660561234533589 1102 20 -0.6638384325086975 1102 21 -0.7126944748993793 1102 22 -0.6603953130038747 1102 23 -0.6285984375211555 1102 24 -0.6340500993935763 1102 25 -0.6395458532221525 1103 1 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2215 14 0.1161359973782794 2215 15 -0.09687627159413381 2215 16 -0.014345549119653356 2215 17 0.14783049246610602 2215 18 0.15579088568512134 2215 19 0.04213644382546988 2215 20 0.09373763586035722 2215 21 0.05531212734480562 2215 22 0.06493054289134648 2215 23 -0.02858530961224659 2215 24 0.13145399521488343 2215 25 0.03248684621095066 2216 1 -0.1370197773878168 2216 2 0.03962363968600968 2216 3 -1.9877820802794375 2216 4 -1.3610155927584344 2216 5 -0.4018250690693037 2216 6 -0.19137167502395158 2216 7 -0.34190192886473625 2216 8 -0.24877301054716838 2216 9 -0.1826506293215715 2216 10 -0.3426984667830837 2216 11 -0.38385497807768587 2216 12 -0.31836579724649994 2216 13 -0.3031831463721133 2216 14 -0.4930886882590215 2216 15 -0.6166977729014604 2216 16 -0.4499110290388373 2216 17 -0.4841447349548176 2216 18 -0.5134929693085795 2216 19 -0.49821776815979746 2216 20 -0.4901195139061727 2216 21 -0.655378040018114 2216 22 -0.6141872894380569 2216 23 -0.787710554135991 2216 24 -0.519120478982579 2216 25 -0.7610992748047778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15338.0 3 8.0 4 221.0 5 1558.0 6 2266.0 7 1670.0 8 1309.0 9 1106.0 10 1053.0 11 872.0 12 860.0 13 814.0 14 811.0 15 844.0 16 828.0 17 875.0 18 960.0 19 1224.0 20 1540.0 21 1993.0 22 2621.0 23 3269.0 24 3825.0 25 4526.0 26 5625.0 27 6108.0 28 7334.0 29 8716.0 30 11272.0 31 14891.0 32 19510.0 33 26318.0 34 35570.0 35 50433.0 36 74755.0 37 132507.0 38 326425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72271639617145 19.764357053682897 10.542550977944236 27.970375572201416 2 13.72330229031658 21.334758685086996 40.914357947961655 24.027581076634767 3 18.30600915410889 24.92760250951983 29.48244076657542 27.283947569795856 4 11.3338226029577 17.811535938585838 39.124380565171364 31.7302608932851 5 12.073353069779465 39.11349668944715 36.392179379094344 12.420970861679036 6 30.732041138209265 39.420467432251 18.061968012304966 11.785523417234772 7 27.642217270701654 32.033624144942415 22.16257300064312 18.161585583712817 8 24.19320895115215 39.429809633426785 20.145158734076578 16.23182268134449 9 25.698551906995714 15.919149980968367 20.248749303364246 38.13354880867167 10 13.160185081746171 31.069130266477185 35.43611380293107 20.334570848845576 11 34.06701896047347 23.338295583856837 24.56911557627057 18.025569879399125 12 22.042735597843734 25.936611028122364 32.79716827953497 19.22348509449893 13 28.395531597064362 22.788465285445216 26.04208612067286 22.77391699681756 14 19.382271680437693 23.34692467263231 28.607149676626577 28.66365397030342 15 21.911302610572967 32.5175265740143 23.55581939412676 22.01535142128597 16 20.73248035331558 29.922712216665587 28.671169708384753 20.673637721634083 17 20.39464625760209 29.8846274748199 28.678814424553227 21.04191184302478 18 20.049775995032793 29.3755009099187 30.535078957042227 20.039644138006285 19 22.06184549278175 28.283804461625188 28.277829302698954 21.376520742894105 20 21.999249203415218 28.82084668551885 29.234174493959202 19.945729617106732 21 21.62419178199647 28.13605473992171 28.0293965955136 22.210356882568213 22 21.279072033045185 29.677146995213384 28.47471277058667 20.569068201154764 23 20.999723323803376 29.082175428296054 28.574805839556383 21.34329540834419 24 21.569934039441243 28.192098761583367 29.580413948618826 20.65755325035656 25 21.39372958086788 28.771701924513348 28.908359909327686 20.926208585291082 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 208.0 1 208.0 2 229.5 3 251.0 4 251.0 5 251.0 6 472.0 7 693.0 8 693.0 9 693.0 10 1202.0 11 1711.0 12 1711.0 13 1711.0 14 2838.5 15 3966.0 16 3966.0 17 3966.0 18 7009.5 19 10053.0 20 10053.0 21 10053.0 22 15982.0 23 21911.0 24 21911.0 25 21911.0 26 31501.0 27 41091.0 28 41091.0 29 41091.0 30 53412.0 31 65733.0 32 65733.0 33 65733.0 34 75161.5 35 84590.0 36 84590.0 37 84590.0 38 90492.0 39 96394.0 40 96394.0 41 96394.0 42 99776.5 43 103159.0 44 103159.0 45 103159.0 46 103140.5 47 103122.0 48 103122.0 49 103122.0 50 96450.0 51 89778.0 52 89778.0 53 89778.0 54 76790.5 55 63803.0 56 63803.0 57 63803.0 58 52785.5 59 41768.0 60 41768.0 61 41768.0 62 32489.0 63 23210.0 64 23210.0 65 23210.0 66 17293.5 67 11377.0 68 11377.0 69 11377.0 70 7987.5 71 4598.0 72 4598.0 73 4598.0 74 3128.5 75 1659.0 76 1659.0 77 1659.0 78 1112.0 79 565.0 80 565.0 81 565.0 82 363.0 83 161.0 84 161.0 85 161.0 86 102.0 87 43.0 88 43.0 89 43.0 90 25.0 91 7.0 92 7.0 93 7.0 94 5.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11625565853310038 2 0.11976281247767437 3 0.018964610218807437 4 0.0 5 0.005715361983750187 6 0.3487669755993012 7 0.022082080391762085 8 0.010001883471562827 9 0.010911145605341266 10 0.00428652148781264 11 0.00558546739321041 12 6.494729526988848E-4 13 6.494729526988848E-4 14 2.59789181079554E-4 15 0.003117470172954647 16 6.494729526988848E-4 17 1.29894590539777E-4 18 7.793675432386617E-4 19 1.29894590539777E-4 20 7.793675432386617E-4 21 0.013768826597216358 22 0.0 23 2.59789181079554E-4 24 1.29894590539777E-4 25 0.006494729526988848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 769855.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.5248494947657 #Duplication Level Percentage of deduplicated Percentage of total 1 92.94978377831279 79.49516268211215 2 5.570407207920284 9.528164761638806 3 0.765367668232264 1.9637386390117244 4 0.2430959837388011 0.8316298968817187 5 0.13681889913295847 0.5850707878192901 6 0.062249031525076395 0.31943034314262503 7 0.04542919434227958 0.2719727506154346 8 0.03186762505264966 0.21803790691067892 9 0.02557089477838391 0.19682522346309966 >10 0.14372140152034116 2.3151193538440675 >50 0.013072910277171619 0.7616935691000724 >100 0.01063949835875436 1.8847488074145091 >500 0.0012159426512274199 0.7881393151426245 >1k 7.599641570171374E-4 0.840265962903171 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1537 0.1996479856596372 No Hit GTATCAACGCAGAGTACTTTTTTTT 1526 0.19821914516369965 No Hit CTGTAGGACGTGGAATATGGCAAGA 1362 0.17691643231517623 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1020 0.1324924823505725 No Hit GTCCTACAGTGTGCATTTCTCATTT 1019 0.13236258776003273 No Hit GGTATCAACGCAGAGTACTTTTTTT 970 0.12599775282358366 No Hit CTTTAGGACGTGAAATATGGCGAGG 948 0.12314007183170857 No Hit TATCAACGCAGAGTACTTTTTTTTT 863 0.11209903163582752 No Hit CTGTAGGACCTGGAATATGGCGAGA 820 0.10651356424261711 No Hit CACACACACACACACACACACACAC 786 0.10209714816426471 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.2989459053977696E-4 13 0.0 0.0 0.0 0.0 1.2989459053977696E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 35 1.00274956E-4 18.97616 19 CGTGCGC 35 0.0021880758 16.264223 10 GTGCGCC 45 6.8248285E-4 14.758276 11 CGTTTCC 45 6.8315543E-4 14.756359 14 GTCTTAG 80 1.2297278E-7 14.301799 1 GGTTATA 80 1.2297278E-7 14.301799 1 GACCTAT 40 0.005150379 14.301799 1 GTTGGAC 40 0.005188428 14.285945 3 TAGACAG 100 6.330083E-10 14.285014 5 CTAAACT 60 2.5062423E-5 14.285013 4 TCCAACG 80 1.3053977E-7 14.2321205 18 GCGTGCG 40 0.005322319 14.231195 9 GGCGAGG 230 0.0 13.613333 19 TAGGACC 275 0.0 13.505831 4 TGTAGGA 815 0.0 13.101814 2 GTAGGAC 765 0.0 12.698617 3 CTACTAA 55 0.0029838015 12.134859 1 CACTAGG 55 0.0029838015 12.134859 1 TGGCGAG 650 0.0 12.115548 18 CCGTTTC 55 0.0030995111 12.073385 13 >>END_MODULE