FastQCFastQC Report
Thu 26 May 2016
SRR1926795_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926795_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721159
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13650.18927864728860072No Hit
CTGTAGGACGTGGAATATGGCAAGA11010.15267090891190432No Hit
GTCCTACAGTGTGCATTTCTCATTT10400.14421230269607674No Hit
GTCCTAAAGTGTGTATTTCTCATTT10150.1407456608043441No Hit
CTTTAGGACGTGAAATATGGCGAGG7910.1096845494544199No Hit
GTATCAACGCAGAGTACTTTTTTTT7560.10483125080599424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2800.012.9234834
GCTACCC603.9664158E-412.7118053
TCTAGTA550.002983513812.1348422
CCTAGTC550.00298507312.1339973
GCATTTC5550.011.95963313
GGCGAGG2300.011.95591819
CTAGACA802.7926928E-511.9032084
ATTTCTC8800.011.74509715
CCTAGAC657.778538E-411.73397353
GCCTCGA658.140766E-411.67049916
GTCCTAC7900.011.4711611
TATGACT1001.8704777E-611.4270784
TGGCGAG6450.011.31993218
ATAGTAC855.124267E-511.2162993
GGCGAGA4400.011.20633119
TGTAGGA7500.011.0600412
GGTCTAC951.296729E-511.04536151
CATTTCT10200.010.96969814
TATGGCG6750.010.95730216
TTTCTCA9550.010.92199916
Produced by FastQC (version 0.11.5)