FastQCFastQC Report
Thu 26 May 2016
SRR1926795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721159
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13270.18400935161316714No Hit
CTGTAGGACGTGGAATATGGCAAGA12210.16931078999222085No Hit
GTATCAACGCAGAGTACTTTTTTTT11590.16071351810072398No Hit
GTCCTACAGTGTGCATTTCTCATTT11100.15391889999292807No Hit
GTCCTAAAGTGTGTATTTCTCATTT10100.1400523324259976No Hit
CTTTAGGACGTGAAATATGGCGAGG9190.12743375594009088No Hit
CTGTAGGACCTGGAATATGGCGAGA8510.11800448999457816No Hit
GGTATCAACGCAGAGTACTTTTTTT7470.1035832597249705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT250.006069453418.9780211
TGCCGGT508.789974E-515.18241510
CGTTTCC653.4091954E-614.59746514
CCAACGA851.7393177E-814.51159719
CGGTTTC602.5943353E-514.23252713
CCGGTTT602.5943353E-514.23252712
GCCGGTT551.9771772E-413.80219611
TAGGACC3250.013.7683934
GGCGAGG2300.012.78864819
ACATTCG604.1298202E-412.65113518
TGTAGGA8550.012.4854542
GTCCTAG1001.3766294E-712.39156151
GTCCTAT853.789908E-612.335491
CATTCGT701.1018845E-412.19930919
GATATAC2450.012.0608611
AGGACCT4900.012.0465965
CTGTAGG8900.011.9952891
GTAGGAC9000.011.743013
GGCTTAG657.791463E-411.7316551
TCCAACG1302.6539055E-911.67797218
Produced by FastQC (version 0.11.5)