FastQCFastQC Report
Thu 26 May 2016
SRR1926794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752971
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11930.1584390368287756No Hit
GTCCTACAGTGTGCATTTCTCATTT9760.12961986583812657No Hit
CTGTAGGACGTGGAATATGGCAAGA9730.12922144411936184No Hit
GTCCTAAAGTGTGTATTTCTCATTT9490.12603407036924397No Hit
GTATCAACGCAGAGTACTTTTTTTT8270.10983158713947815No Hit
CTTTAGGACGTGAAATATGGCGAGG7990.1061129844310073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2350.014.5877874
TAGTACA759.3984636E-713.9664554
CTAGTAC751.4189649E-512.7154423
GACGTGG2600.012.4434777
ACGTGGA2600.012.4327028
TCCAACG701.1088118E-412.19126718
TTAGTAC550.002978895812.13746553
GGACGTG5100.011.9411466
TATGGAC907.134142E-611.6558213
GTCCTAA3450.011.6190651
AGGACGT5250.011.599975
GTAGGAC6900.011.4715393
ACTGGCC855.2723775E-511.185138
TACTAGG600.005699581511.1274982
GTCCTAC6450.011.0979451
TGTAGGA7400.011.0845352
CGTGGAA2950.010.93069659
CTGTAGG7250.010.9265011
GCCTAAG700.001433198110.9076941
AGGACCT4250.010.747035
Produced by FastQC (version 0.11.5)