##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926794_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 752971 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.666843742986117 31.0 30.0 31.0 23.0 34.0 2 28.70791570990118 31.0 30.0 31.0 23.0 34.0 3 28.27807711053945 31.0 28.0 31.0 16.0 34.0 4 32.17402263832206 35.0 32.0 37.0 22.0 37.0 5 32.00253794634853 35.0 32.0 37.0 20.0 37.0 6 31.641680755301334 35.0 32.0 37.0 17.0 37.0 7 31.475339687717057 35.0 32.0 37.0 17.0 37.0 8 31.64220135968052 35.0 32.0 37.0 17.0 37.0 9 33.83012493177028 37.0 34.0 39.0 23.0 39.0 10 34.0007091906594 38.0 34.0 39.0 25.0 39.0 11 34.15088761718579 38.0 35.0 39.0 26.0 39.0 12 34.02702494518381 38.0 35.0 39.0 25.0 39.0 13 34.13464130756696 38.0 35.0 39.0 25.0 39.0 14 35.192291602199816 39.0 36.0 41.0 25.0 41.0 15 34.79014464036464 39.0 34.0 41.0 21.0 41.0 16 34.87580663797145 39.0 34.0 41.0 23.0 41.0 17 35.10728036006699 39.0 36.0 41.0 25.0 41.0 18 35.143522127678224 39.0 36.0 41.0 25.0 41.0 19 34.533070994766064 39.0 34.0 41.0 18.0 41.0 20 34.82546074151594 39.0 34.0 41.0 22.0 41.0 21 34.82299849529397 39.0 35.0 41.0 22.0 41.0 22 34.8510513685122 39.0 35.0 41.0 22.0 41.0 23 34.84916284956525 39.0 35.0 41.0 22.0 41.0 24 33.56496199720839 39.0 33.0 41.0 8.0 41.0 25 34.15145204795404 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.24352095643145333 1101 2 -0.34123494386597386 1101 3 -1.3757286852744883 1101 4 -0.5013392086741923 1101 5 -0.6531244091903332 1101 6 -2.453393426135346 1101 7 -0.7082417158261904 1101 8 -0.12820159495869277 1101 9 -0.26977218202917896 1101 10 -8.68831959525276 1101 11 -3.68607642342133 1101 12 -1.4402919717021874 1101 13 -0.7064245146356782 1101 14 -0.5516771281821562 1101 15 -2.3623396399626344 1101 16 -0.4475760428085209 1101 17 -0.18822703173739086 1101 18 -0.13660172382731162 1101 19 -0.6336354520577459 1101 20 0.0204607335255389 1101 21 -0.06945525047672163 1101 22 0.2261273370381005 1101 23 -0.10963806832027956 1101 24 -0.13295295604156365 1101 25 0.02169443123575121 1102 1 -0.4475537515546293 1102 2 -0.35916688008087405 1102 3 -0.7084968938778822 1102 4 -0.6078550561539409 1102 5 -0.7502965900920451 1102 6 -1.6651054125528368 1102 7 -0.8003245216420147 1102 8 0.01016045402908361 1102 9 -0.004653875809637498 1102 10 -0.23161912680907903 1102 11 -0.3334312737771157 1102 12 -0.258343763147586 1102 13 -0.38166481461527013 1102 14 -0.6306067575497778 1102 15 -0.1191416682318831 1102 16 -0.1373615962689101 1102 17 -0.3165680776003157 1102 18 -0.3703641453908446 1102 19 -0.06958161315325384 1102 20 -0.3307199705954815 1102 21 -0.43283614373157064 1102 22 -0.18600145936006385 1102 23 -0.45328431812818337 1102 24 -3.8899525746840133 1102 25 -1.735754847803321 1103 1 0.2547629367781994 1103 2 0.13973022644566058 1103 3 0.27602832333460015 1103 4 0.38584094674467195 1103 5 -0.1937772778275253 1103 6 0.7545816643755145 1103 7 0.4291385246941992 1103 8 0.7011642217384235 1103 9 0.47885658148108945 1103 10 0.3558913696521131 1103 11 0.33442274768340496 1103 12 0.3237073494160967 1103 13 0.10277594127683187 1103 14 0.3158202837824149 1103 15 0.5646057703867697 1103 16 0.500884448125916 1103 17 0.18973714357817784 1103 18 -0.07738618612323478 1103 19 -1.5154810772522964 1103 20 -0.30178768409289347 1103 21 0.061703248293817126 1103 22 0.06827607809057668 1103 23 0.02954555229101885 1103 24 1.3956956108664897 1103 25 0.7508158022550617 1104 1 -0.013774098648671185 1104 2 0.1312816857390544 1104 3 -1.0846432124738818 1104 4 -0.09398447906370677 1104 5 -0.03456406931425704 1104 6 -1.3287347622154115 1104 7 -0.25687428578605065 1104 8 0.48427245387282625 1104 9 0.4932707870091093 1104 10 0.5153557640526003 1104 11 0.469348213101874 1104 12 0.6946388626178504 1104 13 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19 0.8375168112239422 1111 20 0.39683020705387406 1111 21 0.3863641710726995 1111 22 0.6175936236535478 1111 23 0.3992953152255154 1111 24 0.8278880876861194 1111 25 0.6652934320979327 1112 1 0.5039733304759402 1112 2 0.7196016116194492 1112 3 -0.9429406886656295 1112 4 0.466590333929787 1112 5 0.4991535219173002 1112 6 1.4913321711155447 1112 7 1.5176533353487258 1112 8 1.5421435047617535 1112 9 0.5959810820918605 1112 10 1.1665659982598626 1112 11 0.9171957452693675 1112 12 1.0083799428754858 1112 13 0.7592735655723644 1112 14 0.8577645129755496 1112 15 1.385771015650434 1112 16 1.1002103331437567 1112 17 0.3464309132997272 1112 18 0.7839408586463108 1112 19 1.5827018047232357 1112 20 1.3410125133179918 1112 21 1.2395449935489182 1112 22 1.1946361623847253 1112 23 1.2909370607944979 1112 24 2.2902601866303556 1112 25 1.493507245029356 1113 1 -0.26497430857358495 1113 2 0.3668120741597889 1113 3 -4.7678817217684255 1113 4 -0.9877532276845855 1113 5 0.5168973540005268 1113 6 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2216 10 -3.8752664816727673 2216 11 -2.8003923289813457 2216 12 -2.5890671634202747 2216 13 -2.378746454257321 2216 14 -2.424049805917555 2216 15 -3.455469936976286 2216 16 -2.521032391607193 2216 17 -2.7453224354653187 2216 18 -2.378742732453489 2216 19 -1.7950238872621682 2216 20 -3.0231286176206495 2216 21 -5.534057728537377 2216 22 -4.211634958393304 2216 23 -4.055279098262059 2216 24 -4.027426065889582 2216 25 -3.538801684083733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35007.0 3 3.0 4 261.0 5 1583.0 6 1968.0 7 1633.0 8 1309.0 9 1203.0 10 1205.0 11 1199.0 12 1187.0 13 1164.0 14 1201.0 15 1279.0 16 1303.0 17 1483.0 18 1634.0 19 2039.0 20 2702.0 21 3553.0 22 4408.0 23 5386.0 24 6199.0 25 7223.0 26 8223.0 27 9346.0 28 10323.0 29 12426.0 30 14988.0 31 18408.0 32 24306.0 33 32032.0 34 43530.0 35 61317.0 36 90045.0 37 147010.0 38 194885.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58627556956599 18.404230110784813 12.051475932725051 25.95801838692414 2 14.284194314624136 20.72180387129037 41.69989386801863 23.294107946066866 3 19.39344848820986 24.55227523318929 29.41570005998481 26.638576218616038 4 11.416363769261949 17.203022107634343 39.29789821318219 32.082715909921525 5 12.27836196407033 38.16438276098427 36.58747828119935 12.969776993746049 6 30.763621002964896 38.798471854709305 18.42089882147905 12.01700832084675 7 27.19363566245672 31.383686589412573 22.92419037063581 18.498487377494897 8 24.090187344146294 37.47429079903664 21.636224130949085 16.799297725867984 9 26.00837115762935 15.513208284018953 20.266915330695262 38.21150522765643 10 13.54087091918549 29.829985160070628 35.875163910142035 20.753980010601847 11 34.31060659393791 22.719610861760025 24.729458864597266 18.240323679704797 12 22.76979683463176 25.08690483306435 32.71008919617181 19.43320913613209 13 28.81296946914704 22.308841793463465 25.79362285729466 23.08456588009484 14 20.053876562101504 22.52016386125988 28.455618252146564 28.97034132449205 15 22.71519141186925 31.641103907402908 23.369236756710805 22.27446792401704 16 21.362087631091303 28.83749659610927 28.49079787198714 21.309617900812285 17 21.20317311038673 29.025299338758227 28.33576417392598 21.435763376929064 18 20.844568298404216 28.632889602023226 29.82235693849305 20.700185161079514 19 23.069882610179985 27.56449001117176 27.549080687734044 21.816546690914212 20 22.666677293207517 28.157278117827083 28.409525631216525 20.766518957748872 21 22.464398571293845 27.549614863007054 27.346386858453688 22.639599707245413 22 22.15582145380933 28.713424969050365 27.740570323417053 21.390183253723254 23 21.7136845572862 28.462317057943714 27.94746252161831 21.87653586315177 24 22.829738313362476 27.620884455938587 28.381331133683208 21.16804609701573 25 21.993167315753958 28.02516298024047 28.285506314654143 21.696163389351426 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 239.0 2 298.5 3 358.0 4 358.0 5 358.0 6 523.0 7 688.0 8 688.0 9 688.0 10 1086.5 11 1485.0 12 1485.0 13 1485.0 14 2605.0 15 3725.0 16 3725.0 17 3725.0 18 6485.0 19 9245.0 20 9245.0 21 9245.0 22 14646.0 23 20047.0 24 20047.0 25 20047.0 26 28436.5 27 36826.0 28 36826.0 29 36826.0 30 48307.5 31 59789.0 32 59789.0 33 59789.0 34 68526.0 35 77263.0 36 77263.0 37 77263.0 38 83244.0 39 89225.0 40 89225.0 41 89225.0 42 93600.5 43 97976.0 44 97976.0 45 97976.0 46 99687.5 47 101399.0 48 101399.0 49 101399.0 50 95999.5 51 90600.0 52 90600.0 53 90600.0 54 78628.5 55 66657.0 56 66657.0 57 66657.0 58 56277.5 59 45898.0 60 45898.0 61 45898.0 62 36665.5 63 27433.0 64 27433.0 65 27433.0 66 20827.5 67 14222.0 68 14222.0 69 14222.0 70 10235.5 71 6249.0 72 6249.0 73 6249.0 74 4341.0 75 2433.0 76 2433.0 77 2433.0 78 1648.0 79 863.0 80 863.0 81 863.0 82 561.0 83 259.0 84 259.0 85 259.0 86 161.5 87 64.0 88 64.0 89 64.0 90 41.5 91 19.0 92 19.0 93 19.0 94 11.5 95 4.0 96 4.0 97 4.0 98 4.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23706092266501633 2 0.14303339703653925 3 0.1479472649013043 4 0.06852853562753412 5 0.022178809011236818 6 0.021647580052883843 7 0.30386296417790326 8 0.3010740121465501 9 0.019655471459060176 10 0.03612356916800248 11 0.016999326667295287 12 0.01806178458400124 13 0.0167337121881188 14 0.01846020630276598 15 0.015671254271412843 16 0.02071792937576613 17 0.01925704974029544 18 0.01500721807347162 19 0.023905303125883997 20 0.01846020630276598 21 0.01620248322976582 22 0.017928977344413 23 0.018194591823589488 24 0.01646809770894231 25 0.01580406151100109 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 752971.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.46743845834747 #Duplication Level Percentage of deduplicated Percentage of total 1 94.23698666721575 82.42667831814803 2 4.448462342993144 7.781912124400581 3 0.6549968307323643 1.7187268494748713 4 0.2253238158876326 0.7883398799740609 5 0.11930606048803387 0.5217697751722492 6 0.06825936832777077 0.3582283259048978 7 0.04134872432299329 0.25316669000368136 8 0.02795892811271044 0.1956396659247889 9 0.026599404481130785 0.20939235970337988 >10 0.1260358598186269 2.0598884798552604 >50 0.012437421637344369 0.7506163559498705 >100 0.01091962311367661 1.9529376185438283 >500 0.001213291438973527 0.8244468961136114 >1k 1.5166142987169088E-4 0.15825666083081877 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1193 0.1584390368287756 No Hit GTCCTACAGTGTGCATTTCTCATTT 976 0.12961986583812657 No Hit CTGTAGGACGTGGAATATGGCAAGA 973 0.12922144411936184 No Hit GTCCTAAAGTGTGTATTTCTCATTT 949 0.12603407036924397 No Hit GTATCAACGCAGAGTACTTTTTTTT 827 0.10983158713947815 No Hit CTTTAGGACGTGAAATATGGCGAGG 799 0.1061129844310073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3280723958824443E-4 2 0.0 0.0 0.0 0.0 1.3280723958824443E-4 3 0.0 0.0 0.0 0.0 1.3280723958824443E-4 4 0.0 0.0 0.0 0.0 1.3280723958824443E-4 5 0.0 0.0 0.0 0.0 1.3280723958824443E-4 6 0.0 0.0 0.0 0.0 1.3280723958824443E-4 7 0.0 0.0 0.0 0.0 1.3280723958824443E-4 8 0.0 0.0 0.0 0.0 1.3280723958824443E-4 9 0.0 0.0 0.0 0.0 1.3280723958824443E-4 10 0.0 0.0 0.0 0.0 1.3280723958824443E-4 11 0.0 0.0 0.0 0.0 1.3280723958824443E-4 12 0.0 0.0 0.0 0.0 1.3280723958824443E-4 13 0.0 0.0 0.0 0.0 1.3280723958824443E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 235 0.0 14.587787 4 TAGTACA 75 9.3984636E-7 13.966455 4 CTAGTAC 75 1.4189649E-5 12.715442 3 GACGTGG 260 0.0 12.443477 7 ACGTGGA 260 0.0 12.432702 8 TCCAACG 70 1.1088118E-4 12.191267 18 TTAGTAC 55 0.0029788958 12.1374655 3 GGACGTG 510 0.0 11.941146 6 TATGGAC 90 7.134142E-6 11.655821 3 GTCCTAA 345 0.0 11.619065 1 AGGACGT 525 0.0 11.59997 5 GTAGGAC 690 0.0 11.471539 3 ACTGGCC 85 5.2723775E-5 11.18513 8 TACTAGG 60 0.0056995815 11.127498 2 GTCCTAC 645 0.0 11.097945 1 TGTAGGA 740 0.0 11.084535 2 CGTGGAA 295 0.0 10.9306965 9 CTGTAGG 725 0.0 10.926501 1 GCCTAAG 70 0.0014331981 10.907694 1 AGGACCT 425 0.0 10.74703 5 >>END_MODULE