FastQCFastQC Report
Thu 26 May 2016
SRR1926794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752971
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14750.19589067839266053No Hit
GTCCTACAGTGGACATTTCTAAATT12710.16879800151665866No Hit
CTGTAGGACGTGGAATATGGCAAGA11000.14608796354706888No Hit
GTCCTAAAGTGTGTATTTCTCATTT10000.13280723958824442No Hit
GTCCTACAGTGTGCATTTCTCATTT9740.12935425135895007No Hit
CTTTAGGACGTGAAATATGGCGAGG9040.12005774458777296No Hit
GGTATCAACGCAGAGTACTTTTTTT8810.11700317807724335No Hit
TATCAACGCAGAGTACTTTTTTTTT7840.10412087583718363No Hit
CTGTAGGACCTGGAATATGGCGAGA7620.10119911656624227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAA551.892769E-413.8734981
TAGGACC3450.013.5296014
GTCCTAT500.001453843413.3532431
TCCAACG802.028606E-613.04336918
TAGCACC701.06206186E-412.2476424
TGTAGGA7200.012.0550112
GGACTGA802.7829588E-511.907436
CCTACAC1052.611323E-711.7963863
AAGGCGT657.833628E-411.7242396
CTGTAGG7100.011.6874451
GTCCTAC5850.011.2499841
AAAGGCG600.005752871311.1136015
TCAGTAC951.3141427E-511.03224853
GGTCTAC700.001440997510.9006061
GTAGGAC7350.010.888973
CAAGACA1158.488132E-710.7684584
CTATTAG803.6292546E-410.7302841
CTTGTAC803.6652628E-410.7188313
CCTATAC909.2212955E-510.58653
GTCCTAA3700.010.5691681
Produced by FastQC (version 0.11.5)