FastQCFastQC Report
Thu 26 May 2016
SRR1926793_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926793_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736715
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15120.20523540310703595No Hit
GTCCTAAAGTGTGTATTTCTCATTT12720.17265835499480803No Hit
CTGTAGGACGTGGAATATGGCAAGA11700.15881310954711117No Hit
GTCCTACAGTGTGCATTTCTCATTT10090.13695933977182492No Hit
CTTTAGGACGTGAAATATGGCGAGG9510.1290865531447032No Hit
GTATCAACGCAGAGTACTTTTTTTT9230.1252858975316099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1150.015.667438518
GTATTAA759.213836E-713.9909341
CCAACGA1101.9099389E-1013.79334419
GGCGAGG2700.013.34633719
GTATTAC801.9049639E-613.1165011
ATCCCGT1251.05501385E-1012.89940410
TGGCGAA751.5036379E-512.64389818
GTGTAAT853.7578757E-612.3449421
CTTGGAC853.7982682E-612.3331493
TAAGACC701.0677729E-412.2405344
GTATAAG1251.3151293E-912.210271
CGTTTCC1251.4442776E-912.13814314
TGGCGAG6100.011.8147918
CCGTTTC1302.6775524E-911.6712913
GTCTTAG907.1111554E-611.6591131
TAGGACT1406.530172E-1011.5605044
CCCGTTT1406.9667294E-1011.51497912
ATAGGAC1001.8527971E-611.43619253
TATGGCG6900.011.40699516
ATGGCGA6950.011.18848517
Produced by FastQC (version 0.11.5)