##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926793_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 736715 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.678180843338332 31.0 30.0 33.0 23.0 34.0 2 28.747479011558067 31.0 30.0 31.0 23.0 34.0 3 28.325653746699878 31.0 28.0 31.0 16.0 34.0 4 32.19015494458508 35.0 32.0 37.0 22.0 37.0 5 32.04366546086343 35.0 32.0 37.0 20.0 37.0 6 31.65232281139925 35.0 32.0 37.0 17.0 37.0 7 31.518740625615063 35.0 32.0 37.0 17.0 37.0 8 31.61456601263718 35.0 32.0 37.0 17.0 37.0 9 33.76015962753575 37.0 34.0 39.0 23.0 39.0 10 33.90980772754729 38.0 34.0 39.0 23.0 39.0 11 34.07533849589054 38.0 35.0 39.0 25.0 39.0 12 33.94073556259883 38.0 35.0 39.0 24.0 39.0 13 34.05510543425884 38.0 35.0 39.0 25.0 39.0 14 35.122393327134645 39.0 36.0 41.0 25.0 41.0 15 34.7067061210916 39.0 34.0 41.0 19.0 41.0 16 34.79121098389472 39.0 34.0 41.0 22.0 41.0 17 35.02269398614118 39.0 35.0 41.0 25.0 41.0 18 35.08314341366743 39.0 36.0 41.0 25.0 41.0 19 34.48424288904122 39.0 34.0 41.0 18.0 41.0 20 34.752411719593056 39.0 34.0 41.0 19.0 41.0 21 34.7491445131428 39.0 35.0 41.0 19.0 41.0 22 34.77025036818851 39.0 35.0 41.0 19.0 41.0 23 34.76948209280386 39.0 35.0 41.0 19.0 41.0 24 33.516393720773976 39.0 33.0 41.0 2.0 41.0 25 34.09128631831848 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.06459038755637536 1101 2 -0.10110385946217093 1101 3 -0.8953761370279487 1101 4 -0.22963772947238326 1101 5 -0.1795473205954643 1101 6 -2.3736734148437257 1101 7 -0.6307539930206332 1101 8 0.06251127223708508 1101 9 0.033344923571704044 1101 10 -9.103624978848206 1101 11 -3.761958646284615 1101 12 -1.4727178841603958 1101 13 -0.8189402285155722 1101 14 -0.5490406866461015 1101 15 -2.1726541346726975 1101 16 -0.47835116448095505 1101 17 -0.22747777677943049 1101 18 -0.10416863973235735 1101 19 -0.8487268584923129 1101 20 -0.08612719256846901 1101 21 -0.06177073443882364 1101 22 0.09461913076501105 1101 23 -0.15645758993677816 1101 24 -0.18950283535636459 1101 25 -0.04737514000377985 1102 1 -0.23639415023306043 1102 2 -0.2792895515498408 1102 3 -0.5537873757879126 1102 4 -0.5173052610136182 1102 5 -0.47571288160551006 1102 6 -0.9825438983607704 1102 7 -0.35375243915762766 1102 8 0.2929806334793028 1102 9 0.3269577096444394 1102 10 0.18920450743085127 1102 11 -0.03344344062520577 1102 12 -0.004701261101359933 1102 13 -0.12611929394157073 1102 14 -0.5665980649480602 1102 15 0.2953197298610206 1102 16 0.12798655872764897 1102 17 -0.21071006656214308 1102 18 -0.16429713364972542 1102 19 0.4125723092630338 1102 20 0.007594946158377525 1102 21 -0.02723458256054556 1102 22 -0.2014654619736831 1102 23 -0.5360850631080751 1102 24 -3.6510965820585035 1102 25 -1.9509155459004504 1103 1 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1.742113461586321 1110 8 1.95726163922372 1110 9 1.6477035425853899 1110 10 1.7680019459843876 1110 11 1.3361064228271005 1110 12 1.5440321412905789 1110 13 1.3391316713093957 1110 14 1.20268341165049 1110 15 1.8599444591198946 1110 16 1.7368951188557205 1110 17 1.3087832943946296 1110 18 1.4302739182383988 1110 19 -0.8619121054652865 1110 20 1.1490866120402927 1110 21 1.1966330132582712 1110 22 1.5223785911386614 1110 23 1.4052700550763149 1110 24 0.1322869757152958 1110 25 0.799555308228939 1111 1 0.43306802551052215 1111 2 0.10807034962915552 1111 3 0.831664659161607 1111 4 0.6029630690537076 1111 5 0.8456138291684354 1111 6 0.9761103768764201 1111 7 0.9586768242766581 1111 8 1.26140868105308 1111 9 0.5436525092538105 1111 10 0.6537489154425984 1111 11 0.4486727387271543 1111 12 0.6332762713485351 1111 13 0.5537763231399779 1111 14 0.44919033651873264 1111 15 -0.508372152976591 1111 16 -0.1188557609789953 1111 17 0.316328278315396 1111 18 0.44209551168763994 1111 19 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-5.885397502710777 2216 10 -3.4492623003907923 2216 11 -2.401951349455693 2216 12 -1.8828720586269938 2216 13 -1.567166331762806 2216 14 -1.7068085519327383 2216 15 -2.6518198059881968 2216 16 -1.7213305002668307 2216 17 -2.0285682473235482 2216 18 -1.8498017627027465 2216 19 -1.2827093779541414 2216 20 -2.0972230054337615 2216 21 -4.931996487322451 2216 22 -3.5122866518271323 2216 23 -3.326999249183551 2216 24 -3.551042611036788 2216 25 -2.8881015798606775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35379.0 3 7.0 4 249.0 5 1682.0 6 2051.0 7 1655.0 8 1317.0 9 1233.0 10 1220.0 11 1234.0 12 1155.0 13 1218.0 14 1216.0 15 1179.0 16 1274.0 17 1440.0 18 1623.0 19 2000.0 20 2657.0 21 3557.0 22 4337.0 23 5226.0 24 6177.0 25 7004.0 26 8219.0 27 9019.0 28 9967.0 29 12005.0 30 14416.0 31 17519.0 32 23134.0 33 30796.0 34 41696.0 35 58749.0 36 86477.0 37 141217.0 38 197411.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.348529929948725 18.770721858746477 12.089389183886983 25.791359027417815 2 13.987614651464982 20.628292746578804 42.52950898997972 22.85458361197649 3 19.135441671809286 25.074828526455722 29.576094018883158 26.21363578285183 4 11.251235996566376 17.479028805511188 39.732943247384036 31.536791950538408 5 12.037910702673054 38.507792167239366 36.82133339125925 12.632963738828332 6 30.269583385941264 39.119798094167514 18.742677315868857 11.867941204022369 7 26.70599672143647 31.673129394337124 23.299721703328125 18.321152180898284 8 23.671763312320447 38.235326153552904 21.98959794137269 16.10331259275396 9 25.923336433182413 15.479741241795017 20.49307964349473 38.10384268152784 10 13.466713736778505 30.050645629947454 36.08141318958845 20.401227443685592 11 34.0628847827297 22.728772211339134 25.19559010992144 18.012752896009733 12 22.295395768424054 25.26442258741116 33.28581804359189 19.1543636005729 13 28.702879112047526 22.663911726055368 26.01952713489797 22.61368202699914 14 19.72476503753019 22.976838395508054 28.543443111455634 28.754953455506126 15 22.41911332483502 32.25244597938956 23.49524922721034 21.833191468565076 16 21.015161717748768 28.962980863218718 29.095754501813758 20.92610291721876 17 20.79986858467871 29.367930317297407 28.679628996084695 21.15257210193919 18 20.458337349430302 28.736565307166934 30.444911141309145 20.36018620209362 19 23.021301437745226 27.594933271786797 27.776721832276635 21.607043458191345 20 22.591168636522845 28.497207447169572 28.66079646810625 20.250827448201335 21 22.165144390021396 27.65253537799885 27.607057136961455 22.5752630950183 22 21.98315243583739 29.170993951982403 28.124435755934318 20.721417856245886 23 21.511675264729842 28.658430627206084 28.266494705403204 21.563399402660874 24 22.531763798704603 27.88100606970591 28.8256049002931 20.76162523129639 25 21.914386406955085 28.15417071674097 28.7137597541175 21.217683122186443 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 239.0 2 315.0 3 391.0 4 391.0 5 391.0 6 561.5 7 732.0 8 732.0 9 732.0 10 1171.5 11 1611.0 12 1611.0 13 1611.0 14 2797.0 15 3983.0 16 3983.0 17 3983.0 18 6883.5 19 9784.0 20 9784.0 21 9784.0 22 15347.5 23 20911.0 24 20911.0 25 20911.0 26 30064.5 27 39218.0 28 39218.0 29 39218.0 30 51421.5 31 63625.0 32 63625.0 33 63625.0 34 71469.0 35 79313.0 36 79313.0 37 79313.0 38 84919.0 39 90525.0 40 90525.0 41 90525.0 42 93243.5 43 95962.0 44 95962.0 45 95962.0 46 95950.5 47 95939.0 48 95939.0 49 95939.0 50 90162.0 51 84385.0 52 84385.0 53 84385.0 54 73192.5 55 62000.0 56 62000.0 57 62000.0 58 51748.0 59 41496.0 60 41496.0 61 41496.0 62 33210.0 63 24924.0 64 24924.0 65 24924.0 66 18853.5 67 12783.0 68 12783.0 69 12783.0 70 9203.5 71 5624.0 72 5624.0 73 5624.0 74 3896.5 75 2169.0 76 2169.0 77 2169.0 78 1483.0 79 797.0 80 797.0 81 797.0 82 509.5 83 222.0 84 222.0 85 222.0 86 142.5 87 63.0 88 63.0 89 63.0 90 37.5 91 12.0 92 12.0 93 12.0 94 8.5 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22831081218653076 2 0.1366878643708897 3 0.14021704458304773 4 0.06338950611837685 5 0.019274753466401527 6 0.018188851862660595 7 0.305002612950734 8 0.3047311375497988 9 0.016288524056113966 10 0.030540982605213682 11 0.013302294645826405 12 0.0149311470514378 13 0.013438032346294022 14 0.01465967165050257 15 0.013302294645826405 16 0.016695737157516814 17 0.016152786355646348 18 0.013030819244891172 19 0.019681966567804375 20 0.01533836015284065 21 0.013438032346294022 22 0.0149311470514378 23 0.015609835553775884 24 0.014388196249567336 25 0.013980983148164487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 736715.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.2170340198955 #Duplication Level Percentage of deduplicated Percentage of total 1 93.69740475583099 80.783123334094 2 4.866836555791553 8.392084257999027 3 0.7230535267901146 1.870185915524062 4 0.2557274416500668 0.8819224614625859 5 0.11732720411585619 0.5057801774357998 6 0.06735462080552396 0.3484269380032213 7 0.04942137027476328 0.2982674773602383 8 0.03621682355486313 0.24980056868177497 9 0.022624823192725393 0.17555806358111936 >10 0.13566102785544717 2.18637872418805 >50 0.015132214874712219 0.9103886486573356 >100 0.011821102925414355 2.226433618002616 >500 7.880735210626233E-4 0.4972241560104972 >1k 6.304588168500987E-4 0.6744256589997533 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1512 0.20523540310703595 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1272 0.17265835499480803 No Hit CTGTAGGACGTGGAATATGGCAAGA 1170 0.15881310954711117 No Hit GTCCTACAGTGTGCATTTCTCATTT 1009 0.13695933977182492 No Hit CTTTAGGACGTGAAATATGGCGAGG 951 0.1290865531447032 No Hit GTATCAACGCAGAGTACTTTTTTTT 923 0.1252858975316099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.7147540093523274E-4 0.0 4 0.0 0.0 0.0 2.7147540093523274E-4 0.0 5 0.0 0.0 0.0 2.7147540093523274E-4 0.0 6 0.0 0.0 0.0 2.7147540093523274E-4 0.0 7 0.0 0.0 0.0 2.7147540093523274E-4 0.0 8 0.0 0.0 0.0 2.7147540093523274E-4 0.0 9 0.0 0.0 0.0 2.7147540093523274E-4 0.0 10 0.0 0.0 0.0 2.7147540093523274E-4 0.0 11 0.0 0.0 0.0 2.7147540093523274E-4 0.0 12 0.0 0.0 0.0 2.7147540093523274E-4 0.0 13 0.0 0.0 0.0 2.7147540093523274E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 115 0.0 15.6674385 18 GTATTAA 75 9.213836E-7 13.990934 1 CCAACGA 110 1.9099389E-10 13.793344 19 GGCGAGG 270 0.0 13.346337 19 GTATTAC 80 1.9049639E-6 13.116501 1 ATCCCGT 125 1.05501385E-10 12.899404 10 TGGCGAA 75 1.5036379E-5 12.643898 18 GTGTAAT 85 3.7578757E-6 12.344942 1 CTTGGAC 85 3.7982682E-6 12.333149 3 TAAGACC 70 1.0677729E-4 12.240534 4 GTATAAG 125 1.3151293E-9 12.21027 1 CGTTTCC 125 1.4442776E-9 12.138143 14 TGGCGAG 610 0.0 11.81479 18 CCGTTTC 130 2.6775524E-9 11.67129 13 GTCTTAG 90 7.1111554E-6 11.659113 1 TAGGACT 140 6.530172E-10 11.560504 4 CCCGTTT 140 6.9667294E-10 11.514979 12 ATAGGAC 100 1.8527971E-6 11.4361925 3 TATGGCG 690 0.0 11.406995 16 ATGGCGA 695 0.0 11.188485 17 >>END_MODULE