FastQCFastQC Report
Thu 26 May 2016
SRR1926793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736715
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16810.22817507448606314No Hit
GTCCTACAGTGGACATTTCTAAATT14580.1979055672817847No Hit
CTGTAGGACGTGGAATATGGCAAGA14380.19519081327243235No Hit
CTTTAGGACGTGAAATATGGCGAGG13180.1789022892163184No Hit
GTCCTAAAGTGTGTATTTCTCATTT12760.1732013057966785No Hit
GGTATCAACGCAGAGTACTTTTTTT10420.14143868388725628No Hit
GTCCTACAGTGTGCATTTCTCATTT9650.1309868809512498No Hit
TATCAACGCAGAGTACTTTTTTTTT8830.11985638951290527No Hit
CTGTAGGACCTGGAATATGGCGAGA7520.10207475075164753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG804.3837645E-1016.60195518
AGGACGA350.002132967616.3263055
CCAACGA755.9311787E-815.17893119
GAATAGC456.5753656E-414.8307791
GCCGGTT456.827778E-414.75729511
TGCCGGT500.001513982713.28156510
TAGGACC2950.013.2362994
ACTGTTC2500.012.9055978
CTAACCT604.002511E-412.6982374
CACTGTT2900.012.1096437
AGGACGT6750.011.8516885
GTACAAG1052.589004E-711.804091
CGGTTTC658.108426E-411.67610113
GTATAGG907.1545073E-611.6527551
GTAGGAC8750.011.6468913
ATAAGGC907.236462E-611.6408443
AGGACCT5250.011.60981755
TGTAGGA8550.011.596212
TTAGGAC4700.011.5507793
GGACGTG6850.011.3998316
Produced by FastQC (version 0.11.5)