FastQCFastQC Report
Thu 26 May 2016
SRR1926792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789766
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23940.30312776189402935No Hit
CTGTAGGACGTGGAATATGGCAAGA19190.24298336469283305No Hit
GTCCTACAGTGTGCATTTCTCATTT17270.2186723662451916No Hit
GTCCTAAAGTGTGTATTTCTCATTT16320.20664348680495237No Hit
CTTTAGGACGTGAAATATGGCGAGG13020.1648589582230686No Hit
CTGTAGGACCTGGAATATGGCGAGA11690.14801852700673362No Hit
GTATCAACGCAGAGTACTTTTTTTT8680.10990597214871241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3750.014.2172814
ATAGGAC1051.8981154E-812.7085433
GCCGGTT751.50461365E-512.64336711
TGTCCAC2350.012.51044710
GGCGAGG3800.012.47700719
TCTATAC1155.0513336E-912.432273
TAAGGGT853.8425933E-612.3206484
CGGTTTC701.10847715E-412.19181813
ATTTAGA2650.011.8806411
GGACCTG6500.011.8617476
AGGACCT7200.011.5017455
CCTACAC1501.6552804E-1011.4376893
GTCCTAC11500.011.3656571
TAATACT1451.1696102E-911.1619664
CCGGTTT855.415549E-511.15591112
TCTAGAC600.00572878411.1199753
TGTAGAA5150.011.1045532
GTTATAT1053.2865446E-610.9034461
GCCTATA700.001438003110.9034451
TCCTAGG1053.3243905E-610.8930372
Produced by FastQC (version 0.11.5)